Team:DTU-Denmark/Achievements/Calculator
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Fluorescence per spinach (slope of a standard series). | Fluorescence per spinach (slope of a standard series). | ||
Background fluorescence from your culture (intercept). | Background fluorescence from your culture (intercept). | ||
- | CFU/L or optical density at 600 nm (OD600) for your | + | CFU/L or optical density at 600 nm (OD600) for your examined culture. |
We have found degradation rate of Spinach in <i>E. coli</i> when grown in LB at 37 °C so feel free to use this value. | We have found degradation rate of Spinach in <i>E. coli</i> when grown in LB at 37 °C so feel free to use this value. | ||
You also need a growth rate for your culture, and the copy number of the Spinach sequence per cell. | You also need a growth rate for your culture, and the copy number of the Spinach sequence per cell. | ||
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Latest revision as of 02:38, 18 October 2014