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Team:Paris Saclay/Protocols
From 2014.igem.org
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#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Preparation_of_supercompetent_Ecoli_cells Preparation of supercompetent ''E. coli'' cells] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Preparation_of_supercompetent_Ecoli_cells Preparation of supercompetent ''E. coli'' cells] | ||
Latest revision as of 03:33, 18 October 2014
Protocols
- Preparation of supercompetent E. coli cells
- Transformation of CaCl2 supercompetent E. coli cells
- Extraction of the Plasmid DNA from Bacteria by using NucleoSpin® Plasmid
- Extraction of the Plasmid DNA from Bacteria without NucleoSpin® Plasmid
- Gel Electrophoresis
- Competent E. coli cells
- PCR for the genomic DNA of bacteria
- PCR clean-up
- PCR for bacterial culture
- Transformation of competent cells by electroporation
- BioBrick Assembly
- P1 Phages Stock
- P1 phages transduction
- Preparation and Transformation of competent E. coli by CaCl2
- Cloning in pGEMTeasy
- Electro elution of DNA from agarose gel
