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Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3
From 2014.igem.org
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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | ||
| - | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion# | + | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li> |
</ul> | </ul> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | pSB1A3 with EcoRI & PstI | + | pSB1A3 with EcoRI & PstI |
| - | + | </div> | |
| + | <div class="step-recipe"> | ||
| + | R0010-stem-as-stem-B0015 with EcoRI & PstI | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | 0010-stem-as-stem-B0015 | + | Ligate 0010-stem-as-stem-B0015 with pSB1A3 |
</div> | </div> | ||
</div> | </div> | ||
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<div class="step-recipe"> | <div class="step-recipe"> | ||
R0010-stem-as-stem-B0015(on pSB1A3) | R0010-stem-as-stem-B0015(on pSB1A3) | ||
| - | + | </div> | |
| + | <div class="step-recipe"> | ||
| + | 5.0µL to DH5alpha; | ||
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<div id="footer-content"> | <div id="footer-content"> | ||
<div id="footer-logo"> | <div id="footer-logo"> | ||
| - | <a href="http://igemhokkaidou.com | + | <a href="http://igemhokkaidou.wordpress.com"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a> |
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<div id="footer-twitter"> | <div id="footer-twitter"> | ||
Latest revision as of 15:28, 9 September 2015
Notebook
Lab Documents
H-Stem Vector Exchange to pSB1A3
-
Start
-
Mini-Prep
R0010-stem-as-stem-B0015 (on pSB1A3) -
PCR
R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃ -
Digestion
pSB1A3 with EcoRI & PstIR0010-stem-as-stem-B0015 with EcoRI & PstI -
Ligation
Ligate 0010-stem-as-stem-B0015 with pSB1A3 -
Transformation
R0010-stem-as-stem-B0015(on pSB1A3)5.0µL to DH5alpha; -
Complete!
