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Team:DTU-Denmark/Achievements/Calculator
From 2014.igem.org
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<table> | <table> | ||
| - | <tr style="height:169px | + | <tr style="height:169px;"> |
<td style="width:394px;"> | <td style="width:394px;"> | ||
<table height="80" width="360"> | <table height="80" width="360"> | ||
<tr> | <tr> | ||
| - | <td width=" | + | <td width="71%" colspan="2"> |
Fluorescence: | Fluorescence: | ||
</td> | </td> | ||
| - | <td width=" | + | <td width="14%" style="text-align:right; padding-top: 3px;"> |
| - | <input name="fluorescence" type="number" step="any" min="0 | + | <input name="fluorescence" type="number" step="any" min="0" style="width:90px" /> |
</td> | </td> | ||
| - | <td width=" | + | <td width="15%"></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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</td> | </td> | ||
<td style="text-align:right; padding-top: 3px;"> | <td style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="slope" type="number" step="any" min="0 | + | <input name="slope" type="number" step="any" min="0" style="width:90px" /> |
</td> | </td> | ||
| - | <td width=" | + | <td width="15%"></td> |
</tr> | </tr> | ||
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</td> | </td> | ||
<td style="text-align:right; padding-top: 3px;"> | <td style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="intercept" type="number" step="any" min="0 | + | <input name="intercept" type="number" step="any" min="0" style="width:90px" /> |
</td> | </td> | ||
| - | <td width=" | + | <td width="15%"></td> |
</tr> | </tr> | ||
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</td> | </td> | ||
<td style="text-align:right; padding-top: 3px;"> | <td style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="concentration" type="number" step="any" min="0 | + | <input name="concentration" type="number" step="any" min="0" style="width:90px" /> |
<td>µM</td> | <td>µM</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="7%" style="padding-top:7px"><input type="checkbox" name="conccheck"></td><td colspan="2" width="71%"> | + | <td width="7%" style="padding-top:7px"><input type="checkbox" name="conccheck"></td><td colspan="2" width="71%">Calculate from concentration</td> |
| - | <td width=" | + | <td width="15%"></td> |
</tr> | </tr> | ||
</table> | </table> | ||
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Fluorescence per spinach (slope of a standard series). | Fluorescence per spinach (slope of a standard series). | ||
Background fluorescence from your culture (intercept). | Background fluorescence from your culture (intercept). | ||
| - | CFU/L or optical density at 600 nm (OD600) for your | + | CFU/L or optical density at 600 nm (OD600) for your examined culture. |
We have found degradation rate of Spinach in <i>E. coli</i> when grown in LB at 37 °C so feel free to use this value. | We have found degradation rate of Spinach in <i>E. coli</i> when grown in LB at 37 °C so feel free to use this value. | ||
You also need a growth rate for your culture, and the copy number of the Spinach sequence per cell. | You also need a growth rate for your culture, and the copy number of the Spinach sequence per cell. | ||
| - | When your values are entered to the calculator you will receive your result, the calculator works using the formula derived <a href"https://2014.igem.org/Team:DTU-Denmark/Achievements/Modelling">here</a> | + | When your values are entered to the calculator you will receive your result, the calculator works using the formula derived <a href="https://2014.igem.org/Team:DTU-Denmark/Achievements/Modelling">here</a>. |
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<table height="80px" width="360"> | <table height="80px" width="360"> | ||
<tr> | <tr> | ||
| - | <td width="63%" colspan="2"> | + | <td width="63%" colspan="2">Cell density:</td> |
<td width="16%" style="text-align:right; padding-top: 3px;"> | <td width="16%" style="text-align:right; padding-top: 3px;"> | ||
<input name="CFU" type="number" step="any" min="0" style="width:90px"/> | <input name="CFU" type="number" step="any" min="0" style="width:90px"/> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="7%" style="padding-top:7px"><input type="checkbox" name="CFUcheck"></td><td colspan="2" width="71%"> | + | <td width="7%" style="padding-top:7px"><input type="checkbox" name="CFUcheck"></td><td colspan="2" width="71%">Calculate from OD measurement</td> |
<td width="21%"></td> | <td width="21%"></td> | ||
</tr> | </tr> | ||
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<td width="63%">OD600</td> | <td width="63%">OD600</td> | ||
<td width="16%" style="text-align:right; padding-top: 3px;"> | <td width="16%" style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="OD600" type="number" step="any" min="0 | + | <input name="OD600" type="number" step="any" min="0" style="width:90px" /> |
</td> | </td> | ||
<td width="21%"> | <td width="21%"> | ||
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<td width="63%" colspan="2">Degradation rate:</td> | <td width="63%" colspan="2">Degradation rate:</td> | ||
<td width="16%" style="text-align:right; padding-top: 3px;"> | <td width="16%" style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="degen" type="number" step="any" min="0" placeholder=" | + | <input name="degen" type="number" step="any" min="0" placeholder="0.740" value="0.740" style="width:90px" /> |
</td> | </td> | ||
<td width="21%" style="padding-top:3px"> | <td width="21%" style="padding-top:3px"> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="7%" style="padding-top:7px"><input type="checkbox" name="degcheck"></td><td colspan="2" width="74%"> | + | <td width="7%" style="padding-top:7px"><input type="checkbox" name="degcheck"></td><td colspan="2" width="74%">Calculate from half time</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 193: | Line 193: | ||
<td width="63%">Degradation half time:</td> | <td width="63%">Degradation half time:</td> | ||
<td width="16%" style="text-align:right; padding-top: 3px;"> | <td width="16%" style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="deghalf" type="number" step="any" min="0" placeholder=" | + | <input name="deghalf" type="number" step="any" min="0" placeholder="0.937" value="0.937" style="width:90px" /> |
</td> | </td> | ||
<td width="21%" style="padding-top:4px"> | <td width="21%" style="padding-top:4px"> | ||
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<td width="63%" colspan="2">Growth rate:</td> | <td width="63%" colspan="2">Growth rate:</td> | ||
<td width="16%" style="text-align:right; padding-top: 3px;"> | <td width="16%" style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="growth" type="number" step="any" min="0" placeholder=" | + | <input name="growth" type="number" step="any" min="0" placeholder="0.797" value="0.797" style="width:90px" /> |
<td width="21%" style="padding-top:3px"> | <td width="21%" style="padding-top:3px"> | ||
<select name="growthunit" style="width:80px"> | <select name="growthunit" style="width:80px"> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td width="7%" style="padding-top:7px"><input type="checkbox" name="growthcheck"></td><td colspan="2" width="74%"> | + | <td width="7%" style="padding-top:7px"><input type="checkbox" name="growthcheck"></td><td colspan="2" width="74%">Calculate from doubling time<br></td> |
</tr> | </tr> | ||
</table> | </table> | ||
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<td width="63%">Growth doubling time:</td> | <td width="63%">Growth doubling time:</td> | ||
<td width="16%" style="text-align:right; padding-top: 3px;"> | <td width="16%" style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="grodouble" type="number" step="any" min="0" placeholder=" | + | <input name="grodouble" type="number" step="any" min="0" placeholder="0.870" value="0.870" style="width:90px" /> |
<td width="21%" style="padding-top:4px"> | <td width="21%" style="padding-top:4px"> | ||
<select name="grodoubleunit"> | <select name="grodoubleunit"> | ||
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<td width="63%">Fold-fraction:</td> | <td width="63%">Fold-fraction:</td> | ||
<td width="16%" style="text-align:right; padding-top: 3px;"> | <td width="16%" style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="foldfraction" type="number" step="any" min="0" placeholder=" | + | <input name="foldfraction" type="number" step="any" min="0" placeholder="60" value="60" style="width:90px" /> |
</td> | </td> | ||
<td width="21%">%</td> | <td width="21%">%</td> | ||
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<td width="63%">Copy number:</td> | <td width="63%">Copy number:</td> | ||
<td width="16%" style="text-align:right; padding-top: 3px;"> | <td width="16%" style="text-align:right; padding-top: 3px;"> | ||
| - | <input name="copynumber" type="number" step="any" min="0 | + | <input name="copynumber" type="number" step="any" min="0" style="width:90px" /> |
</td> | </td> | ||
<td width="21%"></td> | <td width="21%"></td> | ||
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</div> | </div> | ||
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Latest revision as of 02:38, 18 October 2014
- Home
- Overview
- Methods
- Achievements
- Team
At the Giant Jamboree, we received a gold medal and an Interlab Study Award. We also won the Overgrad Best Parts Collection.
Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
+45 45 25 25 25
dtuigem14@gmail.com .
+45 45 25 25 25
dtuigem14@gmail.com . 
