"
Team:Sumbawagen/Notebook/protocol7
From 2014.igem.org
(Difference between revisions)
(Created page with "<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <meta name="genera...") |
|||
| (2 intermediate revisions not shown) | |||
| Line 171: | Line 171: | ||
</button> | </button> | ||
<a class="brand" style="padding:0px 15px;width:70px;height:50px;" href="https://igem.org"><img width="70px" height="50px" src="https://static.igem.org/mediawiki/2013/1/17/IGEM_basic_Logo_white_stylized.png"> </a> | <a class="brand" style="padding:0px 15px;width:70px;height:50px;" href="https://igem.org"><img width="70px" height="50px" src="https://static.igem.org/mediawiki/2013/1/17/IGEM_basic_Logo_white_stylized.png"> </a> | ||
| - | <a class="brand" href="https://2014.igem.org/Team:Sumbawagen | + | <a class="brand" href="https://2014.igem.org/Team:Sumbawagen">Sumbawagen</a> |
<div class="nav-collapse collapse"> | <div class="nav-collapse collapse"> | ||
| Line 181: | Line 181: | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/ | + | <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 196: | Line 196: | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li> | + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li> |
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li> | ||
| Line 207: | Line 208: | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| - | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook"> | + | |
| + | <li class="nav-header">Daily Notes</li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li> | ||
<!--<li><a href="#">Improvements</a></li>--> | <!--<li><a href="#">Improvements</a></li>--> | ||
| Line 219: | Line 223: | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li> | ||
| - | |||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li> | ||
| Line 259: | Line 262: | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li> | ||
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li> | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li> | ||
| - | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support"> | + | <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li> |
| Line 299: | Line 302: | ||
<li>Plasmid DNA, 1.5 ul*</li> | <li>Plasmid DNA, 1.5 ul*</li> | ||
<li>Ligation kit (Takara), 5 ul</li> | <li>Ligation kit (Takara), 5 ul</li> | ||
| - | Total volume, 10 ul</li> | + | Total volume, 10 ul</li><br> |
| - | * final quantity insert DNA > plasmid DNA | + | * final quantity insert DNA > plasmid DNA </li> |
| - | </ | + | </ul> |
<p>2. Samples were incubated at 4 oC for overnight (inside a refrigerator).</p> | <p>2. Samples were incubated at 4 oC for overnight (inside a refrigerator).</p> | ||
Latest revision as of 09:22, 19 February 2015
Team:Sumbawagen/Team
From 2014.igem.org
Notebook – Protocol – 7. Ligation
1. Ligation reaction was as follow:
- Insert DNA, 2,5 ul*
- Plasmid DNA, 1.5 ul*
- Ligation kit (Takara), 5 ul Total volume, 10 ul
* final quantity insert DNA > plasmid DNA
2. Samples were incubated at 4 oC for overnight (inside a refrigerator).
Sponsors
