Team:Paris Bettencourt

Here we present a genetic odor palette. It is built of BioBricks to express enzymes to produce volatile compounds with characteristic smells. We included smells with different tonalities to allow exploration of the "odor space" resulting from combinations of smelly units.

Trimethylaminuria (TMAU) is a rare genetic disease causing a strong fish odor to appear in the sweat. Here we engineer skin bacteria to degrade trimethylamine, the odor causing molecule, with the enzyme TMM (trimethylamine monooxygenase).

Isovaleric acid, the cheesy component of foot odor, is produced by B. subtilis through the leucine degradation pathway. We characterize B. subtilis mutants in this pathway, in vitro and in situ (on socks).

Armpit odor comes mainly from aminoacylase enzymes expressed in the genus Corynebacteria. This project is developing CRISPR technology to isolate natural bacterial mutants that lack these enzymes.

2-nonenal is "old people smell," a component of sweat that increases with age. Here we isolate and characterize bacterial species that metabolize 2-nonenal and attenuate its characteristic odor.