Team:ITB Indonesia/Project

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:ITB_Indonesia/Project&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=ITB_Indonesia"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:ITB_Indonesia/Project"style="color:#000000"> Project</a></td>
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<h3>References </h3>
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iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you. </p>
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<li>Conclusions</li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia">HOME</a></li>
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<li>TEAM
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<ul>
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<li><a href="https://igem.org/Team.cgi?id=1387" >OFFICIAL TEAM PROFILE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Team">TEAM MEMBER</a></li>
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<li>PROJECT
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Project">OVERVIEW</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/DegMod">DEGRADATION MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ConMod">CONVERTION MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/RepMod">REPORTER MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SelfMod">SELF REGULATORY MODULE</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/FutureSystem">FUTURE SYSTEM</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Modeling">MODELING</a></li>
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<li>WETLAB
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/protocol">PROTOCOL</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Parts">PARTS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Attributions">ATTRIBUTIONS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Safety">SAFETY</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Data">DATA</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/ProteinModel">PROTEIN MODEL</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Achievement">ACHIEVEMENT</a></li>
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<li>NOTEBOOK
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/nb-wetlab">WETLAB</a></li>
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<li>HUMAN PRACTICE
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Unpas">INTRO SYNBIO TO UNPAS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynBio">SYNBIO CLASS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/SynbiGreen">SYNBIGREEN SURVEY</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Medsos">SOCIAL MEDIA</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/OnMedia">MEDIA</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Biosafety">BIOSAFETY SEMINAR</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/MeetUp">INDONESIA TEAMS MEET UP</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/Kids">FUN WITH KIDS</a></li>
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<li><a href="https://2014.igem.org/Team:ITB_Indonesia/UPI">SHARING SYNBIO IN UPI</a></li>
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<h1>Project Overview</h1>
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<p align="justify">Team ITB_Indonesia tries to create a new method to degrade plastic using synthetic biology approach. We create a novel device which is a synthetic bacteria that able to degrade PET and consume ethylene glycol as the byproduct of degradation reaction. We also establish ompA protein fused with LC Cutinase in <i>E.coli</i> BL21(DE3) thus exposing the enzymes on the surface membrane of bacteria. This condition make the degradation process easily because PET, a high molecular substrate, doesn't need to pass through bacteria cell membrane. We also construct a cassette comprised of two enzyme, glycoaldehyde reductase and glycoaldehyde dehydrogenase which responsible in metabolising ethylene glycol. We use constitutive promoter for both construct, thus making it necessary to create a modul of reporter and self regulatory in order to maintain system efficiency and minimizing severe cytoplasmic stress, in this case, formation of inclusion body aggregate due to strong constitutive expression.</p>
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Latest revision as of 03:40, 17 October 2014


Project Overview

Team ITB_Indonesia tries to create a new method to degrade plastic using synthetic biology approach. We create a novel device which is a synthetic bacteria that able to degrade PET and consume ethylene glycol as the byproduct of degradation reaction. We also establish ompA protein fused with LC Cutinase in E.coli BL21(DE3) thus exposing the enzymes on the surface membrane of bacteria. This condition make the degradation process easily because PET, a high molecular substrate, doesn't need to pass through bacteria cell membrane. We also construct a cassette comprised of two enzyme, glycoaldehyde reductase and glycoaldehyde dehydrogenase which responsible in metabolising ethylene glycol. We use constitutive promoter for both construct, thus making it necessary to create a modul of reporter and self regulatory in order to maintain system efficiency and minimizing severe cytoplasmic stress, in this case, formation of inclusion body aggregate due to strong constitutive expression.



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