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Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 mRFP Assay
From 2014.igem.org
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<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | <li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li> | ||
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li> | ||
| - | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion# | + | <li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li> |
</ul> | </ul> | ||
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<div class="step-tile"> | <div class="step-tile"> | ||
<h2 class="step-title"> | <h2 class="step-title"> | ||
| - | Transformation | + | Double Transformation |
</h2> | </h2> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | Ptrc-stem-asB0034-stem on pHN1257& | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) |
| + | </div> | ||
| + | <div class="step-recipe"> | ||
| + | each of 2.5µL to DH5α | ||
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<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | Ptrc-stem-asB0034-stem(pHN1257)& | + | Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) with pHN1257_up_F Tm:60.1℃ & asB0034_R Tm:68.7℃ |
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| - | + | ||
</div> | </div> | ||
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</ul> | </ul> | ||
<div class="step-recipe"> | <div class="step-recipe"> | ||
| - | Ptrc-stem-asB0034-stem(pHN1257)& | + | Ptrc-stem-asB0034-stem(pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) |
| + | </div> | ||
| + | <div class="step-recipe"> | ||
LB 2 mL | LB 2 mL | ||
| + | </div> | ||
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for 3hours | for 3hours | ||
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Turbidity was controlled 0.1 | Turbidity was controlled 0.1 | ||
</div> | </div> | ||
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<div class="step-recipe"> | <div class="step-recipe"> | ||
LB 2 mL | LB 2 mL | ||
| + | </div> | ||
| + | <div class="step-recipe"> | ||
IPTG 20 µ or not | IPTG 20 µ or not | ||
| + | </div> | ||
| + | <div class="step-recipe"> | ||
for 11 hours, 13hours, 15hours, 17 hours | for 11 hours, 13hours, 15hours, 17 hours | ||
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<div id="footer-content"> | <div id="footer-content"> | ||
<div id="footer-logo"> | <div id="footer-logo"> | ||
| - | <a href="http://igemhokkaidou.com | + | <a href="http://igemhokkaidou.wordpress.com"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a> |
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<div id="footer-twitter"> | <div id="footer-twitter"> | ||
Latest revision as of 15:31, 9 September 2015
Notebook
Lab Documents
140910 asB0034&B0034 mRFP Assay
-
Start
-
Double Transformation
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1)each of 2.5µL to DH5α -
Colony PCR
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(on pSB6A1) with pHN1257_up_F Tm:60.1℃ & asB0034_R Tm:68.7℃ -
Preculture
Ptrc-stem-asB0034-stem(pHN1257) & R0040-B0034-E1010-B0015(pSB6A1)LB 2 mLfor 3hoursTurbidity was controlled 0.1 -
Culture
LB 2 mLIPTG 20 µ or notfor 11 hours, 13hours, 15hours, 17 hours -
Assay
fluoresence imageing 514 nm 610 filter -
Complete!
