Team:LMU-Munich/Notebook/Timeline

From 2014.igem.org

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   <div class="date"><center>21. Oct<br>Heinrich-Wieland-Prize-Symposium</center></div>
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   <div style="float:left; width:23%;height:100%;padding:20px;">09. Sept<br>NEVA FORGET</div>
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   <div class="text"><center>poster session at a fancy symposium</center></div>
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   <div style="float:left; width:57%;height:100%;padding:5px;">HALLO DU</div>
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   <div class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_bacillus.png" ></div>
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   <div style="float:right; width:23%;height:100%;padding:20px;">27. Apr<br>NEVA FORGET</div>
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   <div class="date"><center>17. Oct<br>First ''S. penumoniae'' sensing experiment </center></div>
-
   <div style="float:right; width:57%;height:100%;padding:5px;">HALLO DU</div>
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   <div class="text"><center>Pxyl-comDE PQS-lux was induced with xylose and CSP and the luminescence was measurred.</center></div>
</div>
</div>
 +
<br>
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<div  class="bg-color-2 timeline-item-left biobrick">
 +
  <div class="icon"><center><img src="https://2014.igem.org/File:LMU14_timeline_bacillus.png" ></div>
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  <div class="date"><center>16. Oct<br> ></div>
 +
  <div class="text"><center>Peptides indicate adhesion to ''S.pneumoniae''</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>17. Oct <br> Subtilin Modification BioBrick correctly sequenced</center></div>
 +
  <div  class="text"><center> After struggling for a really long time, the Subtilin Modification BioBrick was correctly sequenced</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://2014.igem.org/File:LMU14_timeline_evaluation.png" ></div>
 +
  <div  class="date"><center>14. Oct <br> Subtilin Immunity BioBrick works! </center></div>
 +
  <div  class="text"><center> The Subtilin Immunity BioBrick was evaluated by spot-on-lawn assays</center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-right biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/8/8b/LMU14_timeline_team.png" ></div>
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  <div class="date"><center>13. Oct<br>Groningen Collaboration Parts received</center></div>
 +
  <div class="text"><center>HALLO DU</center></div>
 +
</div>
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<br>
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<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>08. Oct <br> BioBricks SHIPPED</center></div>
 +
  <div  class="text"><center> Our BioBricks were shipped up to Boston</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
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  <div  class="date"><center>02. Oct<br>BBa_K1351028-BBa_K1351034</center></div>
 +
  <div  class="text"><center>BBa_K1351028-BBa_K1351034 correctly sequenced and packed for submission</center></div>
 +
</div>
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<br>
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<div class="bg-color-2 timeline-item-right  biobrick">
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  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/0/02/LMU14_timeline_world.png" ></div>
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  <div  class="date"><center>30. Sept<br>iGEM talk at freshman week event</center></div>
 +
  <div  class="text"><center>HALLO DU</center></div>
 +
</div>
 +
<br>
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<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>29. Sept<br>BBa_K1351024 PcomC</center></div>
 +
  <div  class="text"><center>QS-dependent promoter PcomC of ''S. pneumoniae'' was cloned succesefully in ''E. coli'' and sequenced!</center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-left biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div class="date"><center>29. Sept<br>BBa_K1351025 PcomAB</center></div>
 +
  <div class="text"><center>QS-dependent promoter PcomAB of ''S. pneumoniae'' was cloned succesefully in ''E. coli'' and sequenced!</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>24. Sept<br>Part sequenced</center></div>
 +
  <div  class="text"><center>Part successfully sequenced:  [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1351023|BBa_K1351023]</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-right  biobrick">
 +
  <div  class="icon"><img src="https://2014.igem.org/File:LMU14_timeline_evaluation.png" ></div>
 +
  <div  class="date"><center>18. Sept <br> First luminescence-assays with synthetic AIP-II done</center></div>
 +
  <div  class="text"><center>Expression of agrCA seems to work!</center></div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-right  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>17. Sept <br> Subtilin Immunity BioBrick correctly sequenced</center></div>
 +
  <div  class="text"><center> The Subtilin Immunity BioBrick was correctly sequenced</center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-right biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/8/8b/LMU14_timeline_team.png" ></div>
 +
  <div class="date"><center>13. Sept - 14. Sept<br>Aachen Meetup</center></div>
 +
  <div class="text"><center>HALLO DU</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>08. Sept <br> pBS2E-PxylA-agrC-IIA constructed</center></div>
 +
  <div  class="text"><center>pBS2E-PxylA-agrC-IIA correctly sequenced</center></div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-right biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/8/8b/LMU14_timeline_team.png" ></div>
 +
  <div class="date"><center>03. Sept<br>MegaWikiDay</center></div>
 +
  <div class="text"><center>We got together for a whole day to discuss and decide on the Wiki's contents and layout</center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-right biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div class="date"><center>25. Aug - 04. Sept<br>LMU Summer School workshop</center></div>
 +
  <div class="text"><center>Robert and Patrick attended the LMU Summer School workshop on "How do innovative products enter the german health care system?" in order to learn more about the regulation of new medical products</center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-left biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div class="date"><center>29. Aug<br>BBa_K1351021</center></div>
 +
  <div class="text"><center>Bba_E1010 successfully improved by deleting a AgeI restriction site</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-right  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>07. Aug <br>SynBioDay</center></div>
 +
  <div  class="text"><center>Presentation of  our Project and eduactional work about SynBio at the Deutsches Museum</center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-left biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div class="date"><center>29. July<br>NEVA FORGET</center></div>
 +
  <div class="text"><center>ComDE mutagenesis to eliminate undesired BioBrick restriction sites started</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center> 24. Jul <br> Subtilin Peptide BioBricks finished </center></div>
 +
  <div  class="text"><center> BioBricks for SpaS were correctly sequenced! :-) </center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-right biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div class="date"><center>10. Jul<br>Open Door Day of the Faculty for Biology of the LMU</center></div>
 +
  <div class="text"><center>"We presented our project at the  [http://www.lsm.bio.lmu.de/news/past-events1/openhouseday2014/index.html Open Doors Day] at the LMU Biology Center</center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-right biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div class="date"><center>09. Jul<br>PANEL DISCUSSION</center></div>
 +
  <div class="text"><center>We invited Experts on SynBio to the LMU's BioCentre. Discussion BLABLA</center></div>
 +
</div>
 +
<br>
 +
<br>
 +
<div class="bg-color-2 timeline-item-right  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>02. Jul - 03. Jul<br>Kongress Nuremberg</center></div>
 +
  <div  class="text"><center>HALLO DU</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-right biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>25. Jun<br>iGEM@School</center></div>
 +
  <div  class="text"><center>The highschool students of the Gymnasium in Neubiberg visited us and had a exciting day at the lab!</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>17. June<br>Succesfull amplification of ''comDE'' of R6 ''S. pneumoniae''</center></div>
 +
  <div  class="text"><center>Now we can start to transferre the quorums sensing system of ''S. pneumoniae first into ''E. coli'' and then into'' to ''B. subtilis''!</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://2014.igem.org/File:LMU14_timeline_bacillus.png" ></div>
 +
  <div  class="date"><center>04. June <br> ''S. pneumoniae'' strain arrived! </center></div>
 +
  <div  class="text"><center> The R6 arrived in our lab today </center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-right biobrick">
 +
  <div class="icon"><center><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div class="date"><center>26. May<br>THE MEETUP ATTENDS CAS</center></div>
 +
  <div class="text"><center>German iGEM Teams attended the Center for Advanced studies</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-left  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/8/8b/LMU14_timeline_team.png" ></div>
 +
  <div  class="date"><center>24. May - 26. May<br>MEETUP OF GERMAN IGEM TEAMS</center></div>
 +
  <div  class="text"><center>The German iGEM Teams came to Munich, and presented their Projects to each other</center></div>
 +
</div>
 +
<br>
 +
<div  class="bg-color-2 timeline-item-left biobrick">
 +
  <div class="icon"><center><img src="https://2014.igem.org/File:LMU14_timeline_bacillus.png" ></div>
 +
  <div class="date"><center>May<br>LABWORK STARTED</center></div>
 +
  <div class="text"><center>We sent our first Labbies to work</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-right  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>04. Apr<br>ANALYTICA</center></div>
 +
  <div  class="text"><center>We visited the Analytica in Munich and approached corporations in search of potential sponsors</center></div>
 +
</div>
 +
<br>
 +
<div class="bg-color-2 timeline-item-right  biobrick">
 +
  <div  class="icon"><img src="https://static.igem.org/mediawiki/2014/c/cb/LMU14_timeline_icon_1.png" ></div>
 +
  <div  class="date"><center>22. Mar - 23. Mar<br>iGEM Weekend</center></div>
 +
  <div  class="text"><center>All the Team Members met for a whole weekend to plan our project</center></div>
 +
</div>
 +
<br>
 +
 +
</div>
</div>

Latest revision as of 03:59, 18 October 2014

Timeline

21. Oct
Heinrich-Wieland-Prize-Symposium
poster session at a fancy symposium

17. Oct
First ''S. penumoniae'' sensing experiment
Pxyl-comDE PQS-lux was induced with xylose and CSP and the luminescence was measurred.

16. Oct
>
Peptides indicate adhesion to ''S.pneumoniae''

17. Oct
Subtilin Modification BioBrick correctly sequenced
After struggling for a really long time, the Subtilin Modification BioBrick was correctly sequenced

14. Oct
Subtilin Immunity BioBrick works!
The Subtilin Immunity BioBrick was evaluated by spot-on-lawn assays

13. Oct
Groningen Collaboration Parts received
HALLO DU

08. Oct
BioBricks SHIPPED
Our BioBricks were shipped up to Boston

02. Oct
BBa_K1351028-BBa_K1351034
BBa_K1351028-BBa_K1351034 correctly sequenced and packed for submission

30. Sept
iGEM talk at freshman week event
HALLO DU

29. Sept
BBa_K1351024 PcomC
QS-dependent promoter PcomC of ''S. pneumoniae'' was cloned succesefully in ''E. coli'' and sequenced!

29. Sept
BBa_K1351025 PcomAB
QS-dependent promoter PcomAB of ''S. pneumoniae'' was cloned succesefully in ''E. coli'' and sequenced!

24. Sept
Part sequenced
Part successfully sequenced: [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1351023|BBa_K1351023]

18. Sept
First luminescence-assays with synthetic AIP-II done
Expression of agrCA seems to work!

17. Sept
Subtilin Immunity BioBrick correctly sequenced
The Subtilin Immunity BioBrick was correctly sequenced

13. Sept - 14. Sept
Aachen Meetup
HALLO DU

08. Sept
pBS2E-PxylA-agrC-IIA constructed
pBS2E-PxylA-agrC-IIA correctly sequenced

03. Sept
MegaWikiDay
We got together for a whole day to discuss and decide on the Wiki's contents and layout

25. Aug - 04. Sept
LMU Summer School workshop
Robert and Patrick attended the LMU Summer School workshop on "How do innovative products enter the german health care system?" in order to learn more about the regulation of new medical products

29. Aug
BBa_K1351021
Bba_E1010 successfully improved by deleting a AgeI restriction site

07. Aug
SynBioDay
Presentation of our Project and eduactional work about SynBio at the Deutsches Museum

29. July
NEVA FORGET
ComDE mutagenesis to eliminate undesired BioBrick restriction sites started

24. Jul
Subtilin Peptide BioBricks finished
BioBricks for SpaS were correctly sequenced! :-)

10. Jul
Open Door Day of the Faculty for Biology of the LMU
"We presented our project at the [http://www.lsm.bio.lmu.de/news/past-events1/openhouseday2014/index.html Open Doors Day] at the LMU Biology Center

09. Jul
PANEL DISCUSSION
We invited Experts on SynBio to the LMU's BioCentre. Discussion BLABLA


02. Jul - 03. Jul
Kongress Nuremberg
HALLO DU

25. Jun
iGEM@School
The highschool students of the Gymnasium in Neubiberg visited us and had a exciting day at the lab!

17. June
Succesfull amplification of ''comDE'' of R6 ''S. pneumoniae''
Now we can start to transferre the quorums sensing system of ''S. pneumoniae first into ''E. coli'' and then into'' to ''B. subtilis''!

04. June
''S. pneumoniae'' strain arrived!
The R6 arrived in our lab today

26. May
THE MEETUP ATTENDS CAS
German iGEM Teams attended the Center for Advanced studies

24. May - 26. May
MEETUP OF GERMAN IGEM TEAMS
The German iGEM Teams came to Munich, and presented their Projects to each other

May
LABWORK STARTED
We sent our first Labbies to work

04. Apr
ANALYTICA
We visited the Analytica in Munich and approached corporations in search of potential sponsors

22. Mar - 23. Mar
iGEM Weekend
All the Team Members met for a whole weekend to plan our project

Hi there!

Welcome to our Wiki! I'm BaKillus, the pathogen-hunting microbe, and I'll guide you on this tour through our project. If you want to learn more about a specific step, you can simply close the tour and come back to it anytime you like. So let's start!

What's the problem?

First of all, what am I doing here? The problem is, pathogenic bacteria all around the world are becoming more and more resistant against antimicrobial drugs. One major reason for the trend is the inappropriate use of drugs. With my BaKillus super powers, I want to reduce this misuse and thus do my part to save global health.

Sensing of pathogens

To combat the pathogenic bacteria, I simply eavesdrop on their communication. Bacteria talk with each other via quorum sensing systems, which I use to detect them and trigger my responses.

Adhesion

The more specific and effective I can use my powers, the lower the danger is of provoking new resistance development. So I catch pathogens whenever I get hold of them and stick to them until my work is done.

Killing

Talking about my work - killing pathogens is finally what I am made for. In response to quorum sensing molecules of the pathogens, I export a range of antimicrobial substances leading to dissipation of biofilms and the killing of the targeted bacteria.

Suicide switch

When the job is done and all the bad guys are finished, you don't need a super hero anymore. So after fulfilling my work I say goodbye to the world by activating my suicide switch.

Application

Of course I'm not only a fictional hero, but a very real one. In two different prototypes, I could be used for diagnosis or treatment of pathogen-caused diseases. However, there is still a whole lot of regulational and economical questions that have to be answered before.

See you!

So now you know my short story - and it is time for me to return to my fight for a safer world. Feel free to take a closer look on my super powers, the process of my development or the plans for a medical application.

Retrieved from "http://2014.igem.org/Team:LMU-Munich/Notebook/Timeline"