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Team:Imperial/Lab-book
From 2014.igem.org
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<h2>Primer Inventory</h2> | <h2>Primer Inventory</h2> | ||
| - | <p>Primers for sequencing, mutagenesis, Gibson Assembly, overlap extension... here is our <a href="https://static.igem.org/mediawiki/2014/d/de/PRIMER-INVENTORY.xls | + | <p>Primers for sequencing, mutagenesis, Gibson Assembly, overlap extension PCR... here is our <a href="https://static.igem.org/mediawiki/2014/d/de/PRIMER-INVENTORY.xls |
">inventory</a></p> | ">inventory</a></p> | ||
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| - | <h2> | + | <h2>Functionalisation cloning</h2> |
| - | <p> | + | <p><a href="https://static.igem.org/mediawiki/2014/9/97/Functionalisation_Labwork_record_GC-CM_collated.pdf">This lab-book </a> is a break-away from the mixed lab-book and contains details of cloning the constructs used in the <a href="https://2014.igem.org/Team:Imperial/Functionalisation">functionalisation</a> part of our project. </p> |
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| + | <p> <a href="https://static.igem.org/mediawiki/2014/c/ce/PCR-Digestion-Phos-Ligation-Test_Functionalisation-mainly.xls | ||
| + | ">This spreadsheet</a> also details specific digestions and ligation set-ups for these constructs. | ||
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| - | <h2> | + | <h2>Interlab study cloning</h2> |
| - | <p> | + | <p>Precise cloning reaction set-ups are detailed <a href="https://static.igem.org/mediawiki/2014/a/af/Interlab-study-cloning-lab-work-record.xls">here</a></p> |
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| - | <h2>Lab-book | + | <h2><i>Gluconacetobacter xylinus</i></h2> |
| - | < | + | <p><a href="https://static.igem.org/mediawiki/2014/7/71/Michael%27s_Lab_notebook_-_Copy.pdf">Lab-book</a> exclusively for work on <i>G.xylinus</i>, with parts including: |
| + | <ul> | ||
| + | <li> Strain growth and isolation </li> | ||
| + | <li> Parts library and characterisation</li> | ||
| + | <li> Genomic DNA extraction and library preparation </li> | ||
| + | </p> | ||
</div> | </div> | ||
Latest revision as of 00:30, 18 October 2014
Lab-books
Introduction
We kept a record of our lab work across a variety of online documents as well as paper based lab-books. Access our online working documents on this page.
General Mixed LabWork
This document contains experimental details for some of the following parts of the project
- Bulk cellulose growth and processing techniques
- Coculture experiments
- G. xylinus isolation and culturing
- Initial cloning work for Acs operon
- Preliminary work for isolating curli genes
Primer Inventory
Primers for sequencing, mutagenesis, Gibson Assembly, overlap extension PCR... here is our inventory
Functionalisation cloning
This lab-book is a break-away from the mixed lab-book and contains details of cloning the constructs used in the functionalisation part of our project.
This spreadsheet also details specific digestions and ligation set-ups for these constructs.
Interlab study cloning
Precise cloning reaction set-ups are detailed here
Gluconacetobacter xylinus
Lab-book exclusively for work on G.xylinus, with parts including:
- Strain growth and isolation
- Parts library and characterisation
- Genomic DNA extraction and library preparation
