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Team:Penn/Protocol
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<title>University of Pennsylvania iGEM</title> | <title>University of Pennsylvania iGEM</title> | ||
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| - | <div style = "text-align: center | + | <div style = "text-align: center;"><img width="300px" src="https://static.igem.org/mediawiki/2014/e/ec/Protocols-header.png"></div><br> |
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| - | E.Coli Specific Protocols | + | <h3>E.Coli Specific Protocols</h3> |
| - | <p><a href="">Gel Extraction</a></p> | + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/7/7e/Mini_Prep.pdf">Miniprep</a></p> |
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/0/0c/Cloning_PCR_Procedures.pdf">Cloning PCR </a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/1/15/Protocol_Colony.pdf">Colony PCR</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c6/Gel_Extraction.pdf">Gel Extraction</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c1/PCR_Cleanup.pdf">PCR Cleanup</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/9/94/Protocol.pdf">Ligation</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c1/PCR_Cleanup.pdf">PCR Cleanup </a></p> | ||
<h3>AMB-1 Specific Protocols</h3> | <h3>AMB-1 Specific Protocols</h3> | ||
| - | <p> Our protocols for working with AMB-1 have been | + | <p style = "text-align: left; text-indent:0px"> Our protocols for working with AMB-1 have been outlined below. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.</p> |
| - | + | <a href="https://2014.igem.org/Team:Penn/Specsheet" style = "font-size: 15px; margin:0 auto; color:#fff"> Click here to view the Spec Sheet! </a></br> | |
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| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/e/ea/Glycerol_Stock_of_Anaerobic_AMB-1.pdf">Glycerol Stock</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a0/MSGM_Media_Preparation.pdf">MSGM Media Preparation </a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/5/5e/EMSGM_Media_Preparation.pdf">EMSGM Media Preparation</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/8/8c/Inoculation.pdf">Inoculation of Aerobic Cultures</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a3/Counting_Cell_Cultures.pdf">Counting Cell Cultures</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/e/ef/Transformation_Protocol_1.pdf">Transformation Protocol 1</a><sup>[7]</sup></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/9/9a/Transformation_Protocol_2.pdf">Transformation Protocol 2</a><sup>[5]</sup></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/7/77/Penn_iGEM_Transformation_Protocol.pdf">Penn iGEM Transformation Protocol</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/5/5d/Plating_Techniques.pdf">Plating Techniques</a></p> | ||
| + | <h3>Materials and Methods</h3> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/4/49/Determining_Magnetic_Properties_of_AMB.pdf">Determining Magnetic Properties of AMB-1</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c7/Determining_the_Correlation_between_OD600_and_Cell_Concentration_for_Aerobic_and_Anaerobic_Cultures.pdf">Determining Correlation between OD600 and Cell Concentration for Aerobic and Anaerobic Cultures</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/6/6f/Optimizing_Transformation.pdf">Optimizing Transformation</a></p> | ||
| + | <p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a8/Determining_Cadmium_Tolerance_of_AMB.pdf">Growth of AMB-1 and E.Coli in Cadmium</a></p> | ||
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Latest revision as of 03:18, 18 October 2014
E.Coli Specific Protocols
AMB-1 Specific Protocols
Our protocols for working with AMB-1 have been outlined below. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.
Click here to view the Spec Sheet!Inoculation of Aerobic Cultures
Penn iGEM Transformation Protocol
Materials and Methods
Determining Magnetic Properties of AMB-1
Determining Correlation between OD600 and Cell Concentration for Aerobic and Anaerobic Cultures
