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Team:SYSU-China/file/Project/Design/Overview.html
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<p> | <p> | ||
| - | 1.The initiate state | + | <h2><b>1.The initiate state</b></h2> |
</p> | </p> | ||
| - | <p>M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An <em>E. coli</em> with pBT-Bait and pRPT-gⅧ. At the temperature of | + | <p>M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An <em>E. coli</em> with pBT-Bait and pRPT-gⅧ. At the temperature of 0℃, M13 phage only attach but not infect into the cell.</p> |
<a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/8/85/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE1_new.png"><img src="https://static.igem.org/mediawiki/2014/8/85/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE1_new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/8/85/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE1_new.png"><img src="https://static.igem.org/mediawiki/2014/8/85/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE1_new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | ||
| - | <p>2.Infection</p> | + | <h2><p><b>2.Infection</b></h2> |
| - | <p>When the temperature rise to | + | </p> |
| + | <p>When the temperature rise to 30℃, the M13 vector infects into the <em>E. coli</em>.</p> | ||
<a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/2/21/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE2new.png"><img src="https://static.igem.org/mediawiki/2014/2/21/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE2new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/2/21/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE2new.png"><img src="https://static.igem.org/mediawiki/2014/2/21/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE2new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | ||
| - | <p>3.Mutagenesis</p> | + | <p><h2><b>3.Mutagenesis</b></h2> |
| + | </p> | ||
<p>When the M13 vector undergo replication, a library of different Prey is generated.</p> | <p>When the M13 vector undergo replication, a library of different Prey is generated.</p> | ||
<a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/7/7f/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE3new.png"><img src="https://static.igem.org/mediawiki/2014/7/7f/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE3new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/7/7f/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE3new.png"><img src="https://static.igem.org/mediawiki/2014/7/7f/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE3new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | ||
| - | <p>4.Selection</p> | + | <p><h2><b>4.Selection</b></h2> |
| - | <p>Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system</p> | + | </p> |
| + | <p>Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system.Only the Prey that can have interaction with Bait can activate transcriping of gⅧ.</p> | ||
<a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/f/f2/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE4new.png"><img src="https://static.igem.org/mediawiki/2014/f/f2/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE4new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/f/f2/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE4new.png"><img src="https://static.igem.org/mediawiki/2014/f/f2/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE4new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | ||
| - | <p>5.Temperature regulated amplification</p> | + | <p><h2><b>5.Temperature regulated amplification</b></h2> |
| - | <p>The temperature rise to | + | </p> |
| + | <p>The temperature rise to 37℃, so that mRNA of gⅧ can be translated into p8.</p> | ||
<a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/6/6d/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE5new.png"><img src="https://static.igem.org/mediawiki/2014/6/6d/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE5new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/6/6d/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE5new.png"><img src="https://static.igem.org/mediawiki/2014/6/6d/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE5new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | ||
| - | <p>6. Rescue and Evolved</p> | + | <p><h2><b>6. Rescue and Evolved</b></h2> |
| - | <p>The p8 compensate M13 deficient, so that progeny phage can be generated</p> | + | </p> |
| + | <p>The p8 compensate M13 deficient, so that progeny phage can be generated.</p> | ||
<a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/f/f6/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE6new.png"><img src="https://static.igem.org/mediawiki/2014/f/f6/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE6new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | <a class="fancybox" rel="group" href="https://static.igem.org/mediawiki/2014/f/f6/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE6new.png"><img src="https://static.igem.org/mediawiki/2014/f/f6/Sysu-%E8%BF%87%E7%A8%8B%E5%9B%BE6new.png" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a> | ||
<!--SYSUCHINA!--> | <!--SYSUCHINA!--> | ||
Latest revision as of 00:33, 18 October 2014
Overview
In order to achieve the goal of integrating the directed evolution processes into a single automatic and controllable system, we come up with the following design.
1.The initiate state
M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An E. coli with pBT-Bait and pRPT-gⅧ. At the temperature of 0℃, M13 phage only attach but not infect into the cell.
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
2.Infection</h2>
When the temperature rise to 30℃, the M13 vector infects into the E. coli.
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
<h2>3.Mutagenesis</h2>
When the M13 vector undergo replication, a library of different Prey is generated.
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
<h2>4.Selection</h2>
Various Prey undergo selection processes with the help of Bacteria Two-Hybrid system.Only the Prey that can have interaction with Bait can activate transcriping of gⅧ.
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
<h2>5.Temperature regulated amplification</h2>
The temperature rise to 37℃, so that mRNA of gⅧ can be translated into p8.
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
<h2>6. Rescue and Evolved</h2>
The p8 compensate M13 deficient, so that progeny phage can be generated.
<a class="fancybox" rel="group" href="
"><img src="" style="width:550px; heigth:auto;margin-left:125px" alt="" /></a>
