Team:Groningen/Template/MODULE/project/MBD/diffusionrates

From 2014.igem.org

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Diffusion rates
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6 bandage designs
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Studying the diffusion rates of Nisin, Aiia and DspB is important to estimate the time taken to reach the threshold concentrations. The threshold concentration is the minimum concentration of the proteins that is required to breakdown biofilm, kill Staphylococcus aureus and quorum quench Pseudomonas aeruginosa population. The diffusion constants for these three proteins were not available directly. They were calculated using number formulas which are listed below. Diffusion rate inside the polyacrylamide gel is different from the diffusions in a solvent.  
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Studying the diffusion rates of Nisin, Aiia and DspB is important to estimate the time taken to reach the threshold concentrations. The threshold concentration is the minimum concentration of the proteins that is required to breakdown biofilm, kill <i>Staphylococcus aureus</i> and quorum quench <i>Pseudomonas aeruginosa</i> population. The diffusion constants for these three proteins were not available directly. They were calculated using number formulas. Diffusion rate inside the polyacrylamide gel is different from the diffusion in a solvent.  
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</html>{{:Team:Groningen/Template/MODULE/newfigurevideo|Figure-movie 1
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</html>{{:Team:Groningen/Template/MODULE/newfigurevideo|Figure-movie 2
|f/fb/Model2lactoaid.png
|f/fb/Model2lactoaid.png
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|2/2b/Design2.mp4|
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|f/f4/Design5.mp4|
Instead of having uniform bacterial distribution, we have single layer of bacteria beneath the top membrane of the bandage.
Instead of having uniform bacterial distribution, we have single layer of bacteria beneath the top membrane of the bandage.
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</html>{{:Team:Groningen/Template/MODULE/newfigurevideo|Figure-movie 3
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|f/ff/Model3lactoaid.png
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|8/89/Model6lactoaid.png
|8/81/Design3.mp4|
|8/81/Design3.mp4|
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Single layer of bacteria are placed in the middle of the bandage.  
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Single layer of bacteria are placed in the bottom of the bandage.  
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|6/6d/Model1lactoaid.png
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|4/4a/Model5lactoaid.png
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|1/1f/Design1.mp4|
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|2/2f/Design4_%281%29.mp4|
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Single layer of bacteria is placed above the bottom membrane.
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Single layer of bacteria is placed in the middle of the gel.
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|4/4a/Model5lactoaid.png
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|8/8c/Model4lactoaid.png
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|f/f4/Design5.mp4|
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|2/2b/Design2.mp4|
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Bacteria are localized to a single lattice in the middle of the bandage.
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Bacteria are localized to a single lattice in the top of the bandage.
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</html>{{:Team:Groningen/Template/MODULE/newfigurevideo|Figure-movie 6
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|8/89/Model6lactoaid.png
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|f/ff/Model3lactoaid.png
|f/fa/Design6.mp4|
|f/fa/Design6.mp4|
Bacteria are localized to two separate lattices in the middle of the bandage.
Bacteria are localized to two separate lattices in the middle of the bandage.
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<div class="item">I'AM A BULLET COPY ME AND EXPLAIN ME.</div>
 
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Latest revision as of 03:24, 18 October 2014

6 bandage designs
 
Studying the diffusion rates of Nisin, Aiia and DspB is important to estimate the time taken to reach the threshold concentrations. The threshold concentration is the minimum concentration of the proteins that is required to breakdown biofilm, kill Staphylococcus aureus and quorum quench Pseudomonas aeruginosa population. The diffusion constants for these three proteins were not available directly. They were calculated using number formulas. Diffusion rate inside the polyacrylamide gel is different from the diffusion in a solvent.
 
figure
 
Figure-movie 1 : The bacteria are uniformly distributed all over the hydrogel in the bandage.
 
 
figure
 
Figure-movie 2 : Instead of having uniform bacterial distribution, we have single layer of bacteria beneath the top membrane of the bandage.
 
 
figure
 
Figure-movie 3 : Single layer of bacteria are placed in the bottom of the bandage.
 
 
figure
 
Figure-movie 4 : Single layer of bacteria is placed in the middle of the gel.
 
 
figure
 
Figure-movie 5 : Bacteria are localized to a single lattice in the top of the bandage.
 
 
figure
 
Figure-movie 6 : Bacteria are localized to two separate lattices in the middle of the bandage.
 
 
 
 
 

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