Team:XMU-China/Gallery

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<div id="Gallery">
<div id="Gallery">
<div id="Gallery_wd">
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<span style="font-size: 32px;">Gallery</span>
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<li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li>
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<li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li><li><img src="https://static.igem.org/mediawiki/2014/8/86/Xmu-parts-004.jpg" width="200px" height="150px"></li>
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<div><a href="https://2014.igem.org/Team:XMU-China/Gallery_1"><p style="font-size:40px; text-align:center;">-----Communication-----</p></a></div>
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<div><a href="https://2014.igem.org/Team:XMU-China/Gallery_2"><p style="font-size:40px; text-align:center;">-----Activities-----</p></a></div>
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<div><a href="https://2014.igem.org/Team:XMU-China/Gallery_3"><p style="font-size:40px; text-align:center;">-----Entertainment-----</p></a></div>
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    <span style="font-size: 27px;">Gallery</span>
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<div><a href="https://2014.igem.org/Team:XMU-China/Gallery_4"><p style="font-size:40px; text-align:center;">-----Practice-----</p></a></div>
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<div><a href="https://2014.igem.org/Team:XMU-China/Gallery_5"><p style="font-size:40px; text-align:center;">-----Team Show-----</p></a></div>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">What </span><span style="font-size: 16px;">measures </span><span style="font-size: 16px;">does school take</span><span style="font-size: 16px;"> to guarantee the safety </span><span style="font-size: 16px;">in</span><span style="font-size: 16px;"> laboratories?</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">School </span><span style="font-size: 16px;">has its own biosafety rules that are regulated by the Ministry of Environment Protection, P.R. China and our institution.</span><span style="font-size: 16px;"> And each</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">laboratory safety standards</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">are implemented strictly.</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">School requires each student to participate in the chemical experimental courses of safety and environmental protection. In the courses, teachers introduce students the matters needing attention and strategies&nbsp;in&nbsp;dealing&nbsp;with the unexpected incidents&nbsp;through various case. Eventually each have to take the course exam, </span><span style="font-size: 16px;">only to meet the requirements of students before they can enter the lab and experimentize.</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Each laboratory is</span><span style="font-size: 16px;"> equipped with safety experimental equipment</span><span style="font-size: 16px;">s</span><span style="font-size: 16px;"> and relevant person in charge</span><span style="font-size: 16px;">.</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">W</span><span style="font-size: 16px;">hat special measures</span><span style="font-size: 16px;"> do you take to</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">guarantee</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">the safety of </span><span style="font-size: 16px;">experiment</span><span style="font-size: 16px;">ers i</span><span style="font-size: 16px;">n view of the </span><span style="font-size: 16px;">experiments related to iGEM</span><span style="font-size: 16px;">?</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Every member of the team</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">has to take the experimental test</span><span style="font-size: 16px;">, </span><span style="font-size: 16px;">which </span><span style="font-size: 16px;">iGEM tutors and postgraduate </span><span style="font-size: 16px;">assigned the</span><span style="font-size: 16px;"> topic. </span><span style="font-size: 16px;">Only the one who pass the exam </span><span style="font-size: 16px;">will be a</span><span style="font-size: 16px;">llowed to enter the laboratory and start</span><span style="font-size: 16px;"> experiment</span><span style="font-size: 16px;">s. And </span><span style="font-size: 16px;">there will be a graduate student </span><span style="font-size: 16px;">give </span><span style="font-size: 16px;">us</span><span style="font-size: 16px;"> a demonstration</span><span style="font-size: 16px;"> and help new players</span><span style="font-size: 16px;"> to correct the mistakes in </span><span style="font-size: 16px;">the experiment.</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">E</span><span style="font-size: 16px;">ach </span><span style="font-size: 16px;">member</span><span style="font-size: 16px;"> shall formulate detailed experimental plan before the experiment, and </span><span style="font-size: 16px;">get </span><span style="font-size: 16px;">familiar with the experimental process, master the characteristics of reagent</span><span style="font-size: 16px;">s</span><span style="font-size: 16px;"> before the experiment. During the experiment, all the players need </span><span style="font-size: 16px;">take</span><span style="font-size: 16px;"> fully protective measures, including </span><span style="font-size: 16px;">wearing </span><span style="font-size: 16px;">experimental suits, goggles, gloves and masks.</span>
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    <span style="font-size: 20px;">Project Safety</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Whether you use the </span><span style="font-size: 16px;">reagent</span><span style="font-size: 16px;">s with potential </span><span style="font-size: 16px;">hazard</span><span style="font-size: 16px;">?</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Strain</span><span style="font-size: 16px;">s</span><span style="font-size: 16px;"> used in the experiments are </span><span style="font-size: 16px; font-style: italic;">Eco.li</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">(</span><span style="font-size: 16px;">k)</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">which is</span><span style="font-size: 16px;"> non-pathogenic bacteria</span><span style="font-size: 16px;">.</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">This year we improve the n</span><span style="font-size: 16px;">ucleic acid gel stain</span><span style="font-size: 16px;"> in the experiment, replacing the EB (e</span><span style="font-size: 16px;">thidium bromide</span><span style="font-size: 16px;">) with </span><span style="font-size: 16px;">SYBR Green I</span><span style="font-size: 16px;">. Although </span><span style="font-size: 16px;">SYBR Green I</span><span style="font-size: 16px;"> has potential </span><span style="font-size: 16px;">hazard</span><span style="font-size: 16px;">, the c</span><span style="font-size: 16px;">arcinogenicity </span><span style="font-size: 16px;">is </span><span style="font-size: 16px;">less than </span><span style="font-size: 16px;">EB.</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">Did </span><span style="font-size: 16px;">the experiments</span><span style="font-size: 16px;"> pollute the environment?</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">To avoid this potential biological pollution to environment, we gathered all culture fluid and culture medium with bacteria and we processed them together to ensure </span><span style="font-size: 16px;">that none of them leaked from our laboratory through sewer and dustbin. </span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">We handled all the biological materials, wastes and equipment strictly following the BSL-1 requirements. All these things that have physically contacted with biological samples were bleached by autoclave before being discarded or reused.</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">What measures do you take to </span><span style="font-size: 16px;">guarantee</span><span style="font-size: 16px;"> the strains </span><span style="font-size: 16px;">won’t leak in the project</span><span style="font-size: 16px;">?</span>
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    <span style="font-family: Calibri;">-</span><span style="font-family: Calibri;"> </span><span style="font-size: 16px;">We designed a </span><span style="font-size: 16px;">black hole system</span><span style="font-size: 16px;"> which can kill the</span><span style="font-size: 16px;"> bacteria</span><span style="font-size: 16px;"> with </span><span style="font-size: 16px;">hyperosmotic pressure</span><span style="font-size: 16px;">. In the gene circuit, the higher </span><span style="font-size: 16px;">osmolality</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">result</span><span style="font-size: 16px;">s</span><span style="font-size: 16px;"> in stronger expression strength of Pomp</span><span style="font-size: 16px;">. Then t</span><span style="font-size: 16px;">he moving tendency of reprogrammed CL-1 will orient to the hyperosmotic pressure spot.</span><span style="font-size: 14px;"> </span><span style="font-size: 16px;">When the bacteria move into where the osmotic pressur</span><span style="font-size: 16px;">e reaches to the critical value, </span><span style="font-size: 16px;">the bacteria </span><span style="font-size: 16px;">will </span><span style="font-size: 16px;">be killed finally.</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">The sources to create hyperosmotic pressure are cheap, accessible, environmentally friendly.</span>
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    <span style="font-size: 20px;">SUPPLEMENTARY</span>
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    <span style="font-size: 16px;">When a freshman or freshwoman comes to our College of Chemisty and Chemical E</span><span style="font-size: 16px;">ngineering in Xiamen University</span><span style="font-size: 16px;">,</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">in the first day, there i</span><span style="font-size: 16px;">s a story about &quot;C3H3&quot; for them</span><span style="font-size: 16px;">,</span><span style="font-size: 16px;"> </span><span style="font-size: 16px;">which was advocated by Prof. Jiaxi Lu, a famous Chinese quantum chemist. &quot;C3H3&quot; consists of three words, Clear Head, Clever Hand and Clean Habit. We think it is very useful when we do synthetic biological experiments, let alone talking about safety in genetic engineering.</span>
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    <span style="font-size: 16px;">Firstly, before doing a genetic engineering experiment, you should keep your head clear. Concretely speaking, you need to find out the safety level of biological materials used in your experiment, including microorganisms, reagents and your constructed gene circuits. Keep every tiny experimental detail in your mind. This can help you avoid many potential safety hazards in your experiment.</span>
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    <span style="font-size: 16px;">In addition, to make your hands clever means to perform a experiment skillfully. For example, when making a gel for SDS-PAGE, it is necessary to use acrylamide, which has strong carcinogenicity. So you have to pay much attention when mixing it and pouring the mixture into a mold to agglomerate.</span>
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    <span style="font-size: 16px;">The last but not the least important, a clean habit is totally useful not only in your lab but also in your life. Imaging a mass, dirty and disorder lab, maybe some tattered gloves or broken flasks which have been contacted with highly toxic reagents are hidden below the experiment rig. Many risks would arise if there&#39;s unclean experimental habit. A clean habit is more than a combination of clear head and clever hand. It&#39;s a kind of decorous attitude to your experiments and a neat working atmosphere you create by yourself. A clean and good habit can improve your whole life.</span>
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    <span style="font-size: 16px;">So this is our &quot;C3H3&quot;. We sincerely hope this proposal can improve safety in synthetic </span><span style="font-size: 16px;">biology and genetic engineering.</span>
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    <span style="font-size: 16px;">Furthermore, we have another reasonable suggestion that every iGEM team should make a data table or survey results about public biosafety or laboratory safety and security of the projects. Every team can submit a survey report attached with questionnaire and analyzing data to the iGEM safety committee. More details are to be discussed.</span>
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