Team:StanfordBrownSpelman/BioBricks
From 2014.igem.org
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<div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499205"><u>Part BBa_K1499205</u></a>: This part encodes the manganese transporter MntH with a UAG stop codon in place of a leucine and a promoter for expression. MntH is involved with manganese homeostasis, a critical process for dealing with free radicals generated upon exposure to radiation that could potentially cause DNA damage.</div> | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499205"><u>Part BBa_K1499205</u></a>: This part encodes the manganese transporter MntH with a UAG stop codon in place of a leucine and a promoter for expression. MntH is involved with manganese homeostasis, a critical process for dealing with free radicals generated upon exposure to radiation that could potentially cause DNA damage.</div> | ||
<div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499250"><u>Part BBa_K1499250</u></a>: GFP (E0040) with two amber stop codons. This GFP contains two stop codons in place of leucine residues. In an amberless cell that also contains supP tRNA, GFP will be translated normally, otherwise, it will be truncated.</div> | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499250"><u>Part BBa_K1499250</u></a>: GFP (E0040) with two amber stop codons. This GFP contains two stop codons in place of leucine residues. In an amberless cell that also contains supP tRNA, GFP will be translated normally, otherwise, it will be truncated.</div> | ||
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499251"><u>Part BBa_K1499251</u></a>: tRNA supP. This is a UAG-leucine tRNA, so a leucine is added every time a UAG codon is encountered.</div> | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499251"><u>Part BBa_K1499251</u></a>: This part encodes the amber-suppressing tRNA supP, a mutant form of the tRNA leuX which normally inserts leucine at UUG. This is a UAG-leucine tRNA, so a leucine is added every time a UAG codon is encountered. 100bp upstream and 50bp downstream are also included to ensure proper splicing and folding.</div> |
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499252"><u>Part BBa_K1499252</u></a>: GFP generator | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499252"><u>Part BBa_K1499252</u></a>: This part encodes a GFP generator followed by the supP tRNA. The GFP produced is E0040 with 2 UAG stop codons in place of leucine residues, which can only be properly translated in amberless cells also containing supP.</div> |
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499253"><u>Part BBa_K1499253</u></a>: aeBlue generator with 3 UAG | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499253"><u>Part BBa_K1499253</u></a>: This part encodes an aeBlue generator followed by the supP tRNA. The aeBlue produced is K864401 with 3 UAG stop codons in place of leucine residues, which can only be properly translated in amberless cells also containing supP.</div> |
</h6> | </h6> | ||
</div> | </div> | ||
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<h5 id="MW"><center><a href="https://2014.igem.org/Team:StanfordBrownSpelman/Material_Waterproofing">Material Waterproofing</a></h5> | <h5 id="MW"><center><a href="https://2014.igem.org/Team:StanfordBrownSpelman/Material_Waterproofing">Material Waterproofing</a></h5> | ||
<h6> | <h6> | ||
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499400"><u>Part BBa_K1499400</u></a>: This is one version of a chitin binding | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499400"><u>Part BBa_K1499400</u></a>: This is one version of a potentially chitin-binding protein (C1) found in the <i> Polistes dominula </i> nest protein samples. It may be responsible for the cellulose-waterproofing capability of oral secretions seen in paper wasp nests. It has been codon-optimized for <i>E. coli</i> expression.</div> |
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499401"><u>Part BBa_K1499401</u></a>: This is another version of a chitin binding | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499401"><u>Part BBa_K1499401</u></a>: This is another version of a potentially chitin-binding protein (C2) found in the <i> Polistes dominula </i> nest protein samples. It may be responsible for the cellulose-waterproofing capability of oral secretions seen in paper wasp nests. It has been codon-optimized for <i>E. coli</i> expression.</div> |
- | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499402"><u>Part BBa_K1499402</u></a>: This is a completely uncharacterized protein from the<i> Polistes dominula </i> | + | <div class="sub"><img src="https://static.igem.org/mediawiki/2014/d/d2/SBS_iGEM_2014_bioBrick.png"><a href="http://parts.igem.org/Part:BBa_K1499402"><u>Part BBa_K1499402</u></a>: This is a completely uncharacterized protein (U1) from the<i> Polistes dominula </i> nest protein samples that may be a major player in the paper wasp's ability to waterproof cellulose. It has been codon-optimized for <i>E. coli</i> expression.</div> |
</h6> | </h6> | ||
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<div id="closing" class="small-8 small-centered columns"> | <div id="closing" class="small-8 small-centered columns"> | ||
<h6> | <h6> | ||
- | Built atop Foundation. Content & Development © Stanford–Brown–Spelman iGEM 2014. | + | Built atop Foundation. Content & Development © Stanford–Brown–Spelman iGEM 2014. |
</h6> | </h6> | ||
</div> | </div> |
Latest revision as of 02:10, 18 October 2014