Team:SYSU-China/file/Project/Design/Overview

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<!--SYSUCHINA-->
 
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<h1>DESIGN OVERVIEW</h1>
 
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<p>In order to achieve the goal of integrating the directed evolution processes into a single automatic and controllable system, we come up with the following design.
 
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</p>
 
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<p>
 
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1.The initiate state
 
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</p>
 
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<p>M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An <em>E. coli</em> with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius</p>
 
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<p>picture1</p>
 
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<p>2.Infection</p>
 
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<p>When the temperature rise to 30 degree Celcius, the M13 vector infects into the <em>E. coli</em>.</p>
 
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<p>picture2</p>
 
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<p>3.Mutagenesis</p>
 
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<p>When the M13 vector undergo replication, a library of different Prey is generated.</p>
 
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<p>picture3</p>
 

Latest revision as of 23:02, 17 October 2014