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Team:Bordeaux/NotebookJune17
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<li>A<sub>600</sub> preculture VPGXG-20 = 1,994<br> | <li>A<sub>600</sub> preculture VPGXG-20 = 1,994<br> | ||
| - | <sub>600</sub> preculture VPGXG-40 = 1,849<br> | + | A<sub>600</sub> preculture VPGXG-40 = 1,849<br> |
| - | <sub>600</sub> preculture VPGXG-60 = 2,177<br> | + | A<sub>600</sub> preculture VPGXG-60 = 2,177<br> |
| - | <div style="margin-left= | + | <div style="margin-left=105px"> - Culture of VPGXG-20, VPGXG-40 and VPGXG-60 at 37°C</div><br> |
| - | T=0, <sub>600</sub> VPGXG-20 = 0,037<br> | + | T=0, A<sub>600</sub> VPGXG-20 = 0,037<br> |
| - | <sub>600</sub> VPGXG-40 = 0,016<br> | + | A<sub>600</sub> VPGXG-40 = 0,016<br> |
| - | <sub>600</sub> VPGXG-60 = 0,018<br> | + | A<sub>600</sub> VPGXG-60 = 0,018<br> |
| - | <div style="margin-left= | + | <div style="margin-left=105px"> - IPTG induction when A<sub>600</sub>= 0,8 and culture at 25°C</div><br> |
T=8h, <sub>600</sub> VPGXG-20 = 1,845<br> | T=8h, <sub>600</sub> VPGXG-20 = 1,845<br> | ||
| - | <sub>600</sub> VPGXG-40 = 1,509<br> | + | A<sub>600</sub> VPGXG-40 = 1,509<br> |
| - | <sub>600</sub> VPGXG-60 = 1,499</li> | + | A<sub>600</sub> VPGXG-60 = 1,499</li> |
</ul> | </ul> | ||
Latest revision as of 21:58, 17 October 2014
17th June
Molecular biology
- Plasmid extraction of VPGXG-20,-40 and VPGXG-60 in pSB6A1 by QIAprep Spin Miniprep Kit from QIAGEN
Production
- A600 preculture VPGXG-20 = 1,994
A600 preculture VPGXG-40 = 1,849
A600 preculture VPGXG-60 = 2,177
- Culture of VPGXG-20, VPGXG-40 and VPGXG-60 at 37°C
T=0, A600 VPGXG-20 = 0,037
A600 VPGXG-40 = 0,016
A600 VPGXG-60 = 0,018
- IPTG induction when A600= 0,8 and culture at 25°C
T=8h, 600 VPGXG-20 = 1,845
A600 VPGXG-40 = 1,509
A600 VPGXG-60 = 1,499
Purification
- We stop protocol after resuspend cell pellet in 20mL PBS and store at -80°C.
Design
- Pattern : Met-M-[(VPGGV)2VPGQQVPGGY]-Spe1-Stop
- We try to design oligo with this pattern
