Team:Bordeaux/NotebookJune12

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<h1>6<sup>th</sup> June </h1>
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<div class="type-exp"> Molecular biology</div>
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<li>We stop protocol after resuspend cell pellet in 20mL PBS and store at -80°C.</li><br>
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Latest revision as of 22:42, 17 October 2014

12th June

Molecular biology

  • Making competent cell: day 3

  • Digestion, ligation and transformation of pSB1C3 and pSB6A1 with our inserts VPGXG-20, VPGXG-40 and VPGXG-60.


Production

A600 preculture VPGXG-20 = 2,349 A600 preculture VPGXG-40 = 2,431 A600 preculture VPGXG-60 = 2,143
- Culture of VPGXG-20, VPGXG-40 and VPGXG-60 at 37°C


T=0, A600 VPGXG-20 = 0,003 A600 VPGXG-40 = 0,005 A600 VPGXG-60 = 0,047
- IPTG induction when A600 = 0,8 and culture at 25°C


Purification

  • We stop protocol after resuspend cell pellet in 20mL PBS and store at -80°C.


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