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Team:British Columbia/Notebook/Protocol
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<html> | <html> | ||
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<div class="container"> | <div class="container"> | ||
<div class="page-header"> | <div class="page-header"> | ||
<h1>Protocols</h1> | <h1>Protocols</h1> | ||
| + | <p> | ||
| - | === | + | |
| + | |||
| + | <ul style="list-style: none; "> | ||
| + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocols/PCR">PCR</a> </font> </b> | ||
| + | </li> | ||
Instruction for colony PCR and regular PCR | Instruction for colony PCR and regular PCR | ||
| + | <br></br> | ||
| + | <li> <b><font size="5"><a href="https://2014.igem.org/Team:British_Columbia/Notebook/Protocol/gelverification"> Gel Verification | ||
| + | </a> </font> </b> | ||
| + | </li> | ||
| + | Make a gel and conduct gel elecrophoresis | ||
| - | + | <br></br> | |
| - | + | ||
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocol/Biobrickdigest">BioBrick Restriction Digest</a> </font> </b> |
| + | </li> | ||
Restriction digest with BioBrick plasmid an inserts | Restriction digest with BioBrick plasmid an inserts | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ligation">BioBrick Ligation</a> </font> </b> |
| + | </li> | ||
Ligate Biobrick insert to vector | Ligate Biobrick insert to vector | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/plates">Making Plates</a> </font> </b> |
| + | </li> | ||
Make LB plates with antibiotics | Make LB plates with antibiotics | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/media">Liquid media</a> </font> </b> |
| + | </li> | ||
Make liquid LB media | Make liquid LB media | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/compcells">Competent cells</a> </font> </b> |
| + | </li> | ||
Preparation of chemically competent cells | Preparation of chemically competent cells | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/transformation">Transformation</a> </font> </b> |
| + | </li> | ||
Transformation of plasmi DNA into chemically competent cells | Transformation of plasmi DNA into chemically competent cells | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly ">CRISPR Repeat-Spacer Array Assembly</a> </font> </b> |
| + | </li> | ||
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides | Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides">Assembling Parts from Oligonucleotides</a> </font> </b> |
| + | </li> | ||
Assemble short parts from annealed oligonucleotides | Assemble short parts from annealed oligonucleotides | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis">(Multi) Site Directed Mutagenesis</a> </font> </b> |
| + | </li> | ||
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. | Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/T4PNK">Oligonucleotide Phosphorylation</a> </font> </b> |
| + | </li> | ||
Phosphorylate oligonucleotides prior to ligation | Phosphorylate oligonucleotides prior to ligation | ||
| + | <br></br> | ||
| + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/ElectrocompCells">Electrocompetent Cell Production</a> </font> </b> | ||
| + | </li> | ||
| - | |||
Make electrocompetent cells | Make electrocompetent cells | ||
| - | + | <br></br> | |
| - | == | + | <li> <b><font size="5"><a href="Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries">Make Combinatorial Part Libraries</a> </font> </b> |
| + | </li> | ||
Make combinatorial part libraries from compatible parts | Make combinatorial part libraries from compatible parts | ||
| + | <br></br> | ||
| - | </ | + | </ul> |
| - | + | ||
| - | + | ||
<br> | <br> | ||
Latest revision as of 23:26, 17 October 2014
Protocols
- PCR Instruction for colony PCR and regular PCR
- Gel Verification Make a gel and conduct gel elecrophoresis
- BioBrick Restriction Digest Restriction digest with BioBrick plasmid an inserts
- BioBrick Ligation Ligate Biobrick insert to vector
- Making Plates Make LB plates with antibiotics
- Liquid media Make liquid LB media
- Competent cells Preparation of chemically competent cells
- Transformation Transformation of plasmi DNA into chemically competent cells
- CRISPR Repeat-Spacer Array Assembly Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
- Assembling Parts from Oligonucleotides Assemble short parts from annealed oligonucleotides
- (Multi) Site Directed Mutagenesis Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
- Oligonucleotide Phosphorylation Phosphorylate oligonucleotides prior to ligation
- Electrocompetent Cell Production Make electrocompetent cells
- Make Combinatorial Part Libraries Make combinatorial part libraries from compatible parts
