Team:Cooper Union/Notebook/Biohack May

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<ul class="menu">
<ul class="menu">
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan</a> </li>
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan Timer</a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
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<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
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<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
 
</ul>
</ul>
</li>
</li>
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<li class="menu-safety"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li>
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<li class="menu-safety"> Social
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<li class="menu-outreach"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
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<ul class="menu">
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<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li>
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<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Ethics">Ethics</a> </li>
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<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
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</ul>
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</li>
<li class="menu-notebook">  Notebook  
<li class="menu-notebook">  Notebook  
<ul class="menu">
<ul class="menu">
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<h3>5/28/14</h3>
<h3>5/28/14</h3>
<img src=" https://static.igem.org/mediawiki/2014/0/06/CU_528_Gel.jpg " width="350" />
<img src=" https://static.igem.org/mediawiki/2014/0/06/CU_528_Gel.jpg " width="350" />
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             <span>Projects</span>
             <span>Projects</span>
         </a>
         </a>
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         <a href="https://2014.igem.org/Team:Cooper_Union/Safety" class="red" >
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         <a href="https://2014.igem.org/Team:Cooper_Union/Social" class="red" >
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             <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" />
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             <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Social" />
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             <span>Safety</span>
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             <span>Social</span>
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        </a>
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        <a href="https://2014.igem.org/Team:Cooper_Union/Outreach"  class="yellow">
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            <img src="https://static.igem.org/mediawiki/2014/7/74/CU_2014_logoO.png" height="100" style="vertical-align: top;" title="Outreach" />
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            <span>Outreach</span>
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         </a>
         </a>
         <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >
         <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >

Latest revision as of 00:45, 18 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


5/19/14

  • Calibrated Nanodrop.
  • Nanodropped all of our samples. Very low concentration of DNA.
  • Ordered primers to PCR our g-blocks.

5/22/14

  • Struck out plates with pACYC184 with spectinomycin to grow it.
  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII/EcoRI.

5/23/14

  • Ran gel of digests from 5/22/14. Everything as it should be.
  • Purified the digested plasmids.
  • Nanodropped plasmids; results:
    Single digest I: 52.7ng/μL
    Single digest II: 64.5ng/μL
    Double digest I: 26.3ng/μL
    Double digest II: 41.9ng/μL
  • Phosphatase treated the four digested plasmids.

5/27/14

  • Purified phosphatase treated plasmids from 5/23/14.
  • Nanodropped the purified plasmids; results:
    Single digest I: 43.4ng/μL
    Single digest II: 53.6ng/μL
    Double digest I: 43.7ng/μL
    Double digest II: 16.5ng/μL

5/28/14



Projects Social Notebook Team

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