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Team:NCTU Formosa/biobricks
From 2014.igem.org
(→PBAN Aedes aegypti 2 (BBa_K1415109)) |
(→PBAN Spodoptera litura 2 (BBa_K1415105)) |
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===Brief Information=== | ===Brief Information=== | ||
| - | + | ||
=====PBAN ''Bombyx mori'' 1 (BBa_K1415001)===== | =====PBAN ''Bombyx mori'' 1 (BBa_K1415001)===== | ||
[[File:Pbanbm.png|176px|link=|frameless|left]] | [[File:Pbanbm.png|176px|link=|frameless|left]] | ||
| Line 21: | Line 21: | ||
LSEDMPATPADQEMYQPDPEEMESRTRYFSPRL | LSEDMPATPADQEMYQPDPEEMESRTRYFSPRL | ||
<P>PBAN(BM)+pSB1C3 This biobrick is the PBAN of ''Bombyx mori'' on the pSB1C3 backbone.</P> | <P>PBAN(BM)+pSB1C3 This biobrick is the PBAN of ''Bombyx mori'' on the pSB1C3 backbone.</P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Bombyx mori'' 2 (BBa_K1415101)===== | =====PBAN ''Bombyx mori'' 2 (BBa_K1415101)===== | ||
[[File:HALFBM.png|457px|link=|frameless|left]] | [[File:HALFBM.png|457px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(BM) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(BM) | ||
| - | This circuit is responsible for producing the ''Bombyx mori''’s PBAN. As ''Bombyx mori'' PBAN is ingested by the female ''Bombyx mori'', the female ''Bombyx mori'' will produce its own sex pheromone and attract male ''Bombyx moris''. </P> | + | This circuit is responsible for '''producing the ''Bombyx mori''’s PBAN'''. As ''Bombyx mori'' PBAN is ingested by the female ''Bombyx mori'', the female ''Bombyx mori'' will produce its own sex pheromone and attract male ''Bombyx moris''. </P> |
| + | <h2></h2> | ||
=====PBAN ''Bombyx mori'' 3 (BBa_K1415201)===== | =====PBAN ''Bombyx mori'' 3 (BBa_K1415201)===== | ||
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The above circuit is constructed mainly to test whether it produces ''Bombyx mori''’s PBAN. | The above circuit is constructed mainly to test whether it produces ''Bombyx mori''’s PBAN. | ||
If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light. </p> | If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light. </p> | ||
| - | + | <h1></h1> | |
=====PBAN ''Mamestra brassicae'' 1 (BBa_K1415002)===== | =====PBAN ''Mamestra brassicae'' 1 (BBa_K1415002)===== | ||
[[File:MB.png|276px|link=|frameless|left]] | [[File:MB.png|276px|link=|frameless|left]] | ||
| Line 38: | Line 39: | ||
LADDMPATPADQEMYRPDPEQIDSRTKYFSPRL | LADDMPATPADQEMYRPDPEQIDSRTKYFSPRL | ||
<P>PBAN(MB)+pSB1C3 This biobrick is the PBAN of ''Mamestra brassicae'' on the pSB1C3 backbone.</P> | <P>PBAN(MB)+pSB1C3 This biobrick is the PBAN of ''Mamestra brassicae'' on the pSB1C3 backbone.</P> | ||
| - | + | <h1></h1> | |
=====''PBAN Mamestra brassicae'' 2 (BBa_K1415102)===== | =====''PBAN Mamestra brassicae'' 2 (BBa_K1415102)===== | ||
[[File:HALFMB.png|457px|link=|frameless|left]] | [[File:HALFMB.png|457px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(MB) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(MB) | ||
| - | This circuit is responsible for producing the ''Mamestra brassicae’s'' PBAN. As ''Mamestra brassicae’s'' PBAN is ingested by the female ''Mamestra brassicae'', the female ''Mamestra brassicae'' will produce its own sex pheromone and attract male ''Mamestra brassicaes''. </P> | + | This circuit is responsible for '''producing the ''Mamestra brassicae’s'' PBAN'''. As ''Mamestra brassicae’s'' PBAN is ingested by the female ''Mamestra brassicae'', the female ''Mamestra brassicae'' will produce its own sex pheromone and attract male ''Mamestra brassicaes''. </P> |
| - | + | <h1></h1> | |
=====PBAN ''Mamestra brassicae'' 3 (BBa_K1415202)===== | =====PBAN ''Mamestra brassicae'' 3 (BBa_K1415202)===== | ||
[[File:ALLMB.png|780px|link=|frameless|left]] | [[File:ALLMB.png|780px|link=|frameless|left]] | ||
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If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light. | If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light. | ||
</P> | </P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Agrotis ipsilon'' 1 (BBa_K1415003)===== | =====PBAN ''Agrotis ipsilon'' 1 (BBa_K1415003)===== | ||
[[File:AI.png|276px|link=|frameless|left]] | [[File:AI.png|276px|link=|frameless|left]] | ||
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LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL | LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL | ||
<P>PBAN(AI)+pSB1C3 This biobrick is the PBAN of ''Agrotis ipsilon'' on the pSB1C3 backbone.</P> | <P>PBAN(AI)+pSB1C3 This biobrick is the PBAN of ''Agrotis ipsilon'' on the pSB1C3 backbone.</P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Agrotis ipsilon'' 2 (BBa_K1415103)===== | =====PBAN ''Agrotis ipsilon'' 2 (BBa_K1415103)===== | ||
[[File:HALFAI.png|457px|link=|frameless|left]] | [[File:HALFAI.png|457px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AI) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AI) | ||
| - | This circuit is responsible for producing the ''Agrotis ipsilon''’s PBAN. As ''Agrotis ipsilon''’s PBAN is ingested by the female ''Agrotis ipsilon'', the female ''Agrotis ipsilon'' will produce its own sex pheromone and attract male ''Agrotis ipsilons''.</P> | + | This circuit is responsible for '''producing the ''Agrotis ipsilon''’s PBAN'''. As ''Agrotis ipsilon''’s PBAN is ingested by the female ''Agrotis ipsilon'', the female ''Agrotis ipsilon'' will produce its own sex pheromone and attract male ''Agrotis ipsilons''.</P> |
| - | + | <h1></h1> | |
=====PBAN ''Agrotis ipsilon'' 3 (BBa_K1415203)===== | =====PBAN ''Agrotis ipsilon'' 3 (BBa_K1415203)===== | ||
[[File:ALLAI.png|780px|link=|frameless|left]] | [[File:ALLAI.png|780px|link=|frameless|left]] | ||
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If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light. | If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light. | ||
</P> | </P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Lymantria dispar'' 1 (BBa_K1415004)===== | =====PBAN ''Lymantria dispar'' 1 (BBa_K1415004)===== | ||
[[File:LD.png|276px|link=|frameless|left]] | [[File:LD.png|276px|link=|frameless|left]] | ||
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LADDMPATMADQEVYRPEPEQIDSRNKUFSPRL | LADDMPATMADQEVYRPEPEQIDSRNKUFSPRL | ||
<P>PBAN(AI)+pSB1C3 This biobrick is the PBAN of ''Lymantria dispar'' on the pSB1C3 backbone.</P> | <P>PBAN(AI)+pSB1C3 This biobrick is the PBAN of ''Lymantria dispar'' on the pSB1C3 backbone.</P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Lymantria dispar'' 2 (BBa_K1415104)===== | =====PBAN ''Lymantria dispar'' 2 (BBa_K1415104)===== | ||
[[File:HALFLD.png|457px|link=|frameless|left]] | [[File:HALFLD.png|457px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(LD) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(LD) | ||
| - | This circuit is responsible for producing ''Lymantria dispar''’s PBAN. As ''Lymantria dispar'' PBAN is ingested by the female ''Lymantria dispar'', the female ''Lymantria dispar'' will produce its own sex pheromone and attract male ''Lymantria dispars''.</P> | + | This circuit is responsible for''' producing ''Lymantria dispar''’s PBAN'''. As ''Lymantria dispar'' PBAN is ingested by the female ''Lymantria dispar'', the female ''Lymantria dispar'' will produce its own sex pheromone and attract male ''Lymantria dispars''.</P> |
| - | + | <h1></h1> | |
=====PBAN ''Lymantria dispar'' 3 (BBa_K1415204)===== | =====PBAN ''Lymantria dispar'' 3 (BBa_K1415204)===== | ||
[[File:ALLLD.png|780px|link=|frameless|left]] | [[File:ALLLD.png|780px|link=|frameless|left]] | ||
| Line 87: | Line 88: | ||
</P> | </P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Spodoptera litura'' 1 (BBa_K1415005)===== | =====PBAN ''Spodoptera litura'' 1 (BBa_K1415005)===== | ||
[[File:SL.png|276px|link=|frameless|left]] | [[File:SL.png|276px|link=|frameless|left]] | ||
| Line 93: | Line 94: | ||
LADDMPATPADQELYRPDPDQIDSRTKUFSPRL | LADDMPATPADQELYRPDPDQIDSRTKUFSPRL | ||
<P>PBAN(SL)+pSB1C3 This biobrick is the PBAN of ''Spodoptera litura'' on the pSB1C3 backbone.</P> | <P>PBAN(SL)+pSB1C3 This biobrick is the PBAN of ''Spodoptera litura'' on the pSB1C3 backbone.</P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Spodoptera litura'' 2 (BBa_K1415105)===== | =====PBAN ''Spodoptera litura'' 2 (BBa_K1415105)===== | ||
[[File:HALFSL.png|457px|link=|frameless|left]] | [[File:HALFSL.png|457px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(SL) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(SL) | ||
| - | This circuit is responsible for producing the ''Spodoptera litura'' PBAN. As ''Spodoptera litura'' PBAN is ingested by the female ''Spodoptera litura'', the female ''Spodoptera litura'' will produce its own sex pheromone and attract male ''Spodoptera lituras''.</P> | + | This circuit is responsible for '''producing the ''Spodoptera litura''’s PBAN'''. As ''Spodoptera litura'' PBAN is ingested by the female ''Spodoptera litura'', the female ''Spodoptera litura'' will produce its own sex pheromone and attract male ''Spodoptera lituras''.</P> |
| + | <h1></h1> | ||
=====PBAN ''Spodoptera litura'' 3 (BBa_K1415205)===== | =====PBAN ''Spodoptera litura'' 3 (BBa_K1415205)===== | ||
| Line 105: | Line 107: | ||
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light. | If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light. | ||
</P> | </P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Helicoverpa armigera Hubner'' 1 (BBa_K1415006)===== | =====PBAN ''Helicoverpa armigera Hubner'' 1 (BBa_K1415006)===== | ||
[[File:HAH.png|276px|link=|frameless|left]] | [[File:HAH.png|276px|link=|frameless|left]] | ||
| Line 111: | Line 113: | ||
LSDDMPARPADQEMYRQDPEQIDSRTKYFSPRL | LSDDMPARPADQEMYRQDPEQIDSRTKYFSPRL | ||
<P>PBAN(HAH)+pSB1C3 This biobrick is the PBAN of ''Helicoverpa armigera Hubner'' on the pSB1C3 backbone.</P> | <P>PBAN(HAH)+pSB1C3 This biobrick is the PBAN of ''Helicoverpa armigera Hubner'' on the pSB1C3 backbone.</P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Helicoverpa armigera Hubner'' 2 (BBa_K1415106)===== | =====PBAN ''Helicoverpa armigera Hubner'' 2 (BBa_K1415106)===== | ||
[[File:HALFHAH.png|457px|link=|frameless|left]] | [[File:HALFHAH.png|457px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(HAH) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(HAH) | ||
| - | This circuit is responsible for producing the ''Helicoverpa armigera Hubner''’s PBAN. As ''Helicoverpa armigera Hubner''’s PBAN is ingested by the female ''Helicoverpa armigera Hubner'', the female ''Helicoverpa armigera Hubner'' will produce its own sex pheromone and attract male ''Helicoverpa armigera Hubner''s.</P> | + | This circuit is responsible for''' producing the ''Helicoverpa armigera Hubner''’s PBAN'''. As ''Helicoverpa armigera Hubner''’s PBAN is ingested by the female ''Helicoverpa armigera Hubner'', the female ''Helicoverpa armigera Hubner'' will produce its own sex pheromone and attract male ''Helicoverpa armigera Hubner''s.</P> |
| - | + | <h1></h1> | |
=====PBAN ''Helicoverpa armigera Hubner'' 3 (BBa_K1415206)===== | =====PBAN ''Helicoverpa armigera Hubner'' 3 (BBa_K1415206)===== | ||
[[File:ALLHAH.png|780px|link=|frameless|left]] | [[File:ALLHAH.png|780px|link=|frameless|left]] | ||
| Line 123: | Line 125: | ||
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light. | If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light. | ||
</P> | </P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Adoxophyes sp.'' 1 (BBa_K1415007)===== | =====PBAN ''Adoxophyes sp.'' 1 (BBa_K1415007)===== | ||
[[File:AS.png|276px|link=|frameless|left]] | [[File:AS.png|276px|link=|frameless|left]] | ||
| Line 129: | Line 131: | ||
QSEAVTSSDEQVYRQDMSPVDGRLKYFSPRL | QSEAVTSSDEQVYRQDMSPVDGRLKYFSPRL | ||
<P>PBAN(AS)+pSB1C3 This biobrick is the PBAN of ''Adoxophyes sp.'' on the pSB1C3 backbone.</P> | <P>PBAN(AS)+pSB1C3 This biobrick is the PBAN of ''Adoxophyes sp.'' on the pSB1C3 backbone.</P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Adoxophyes sp.'' 2 (BBa_K1415107)===== | =====PBAN ''Adoxophyes sp.'' 2 (BBa_K1415107)===== | ||
[[File:HALFAS.png|457px|link=|frameless|left]] | [[File:HALFAS.png|457px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AS) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AS) | ||
| - | This circuit is responsible for producing the ''Adoxophyes sp.''’s PBAN. As ''Adoxophyes sp.''’s PBAN is ingested by the female ''Adoxophyes sp.'', the female ''Adoxophyes sp.'' will produce its own sex pheromone and attract male ''Adoxophyes sp.''</P> | + | This circuit is responsible for '''producing the ''Adoxophyes sp.''’s PBAN'''. As ''Adoxophyes sp.''’s PBAN is ingested by the female ''Adoxophyes sp.'', the female ''Adoxophyes sp.'' will produce its own sex pheromone and attract male ''Adoxophyes sp.''</P> |
| - | + | <h1></h1> | |
=====PBAN ''Adoxophyes sp.'' 3 (BBa_K1415207)===== | =====PBAN ''Adoxophyes sp.'' 3 (BBa_K1415207)===== | ||
[[File:ALLAS.png|780px|link=|frameless|left]] | [[File:ALLAS.png|780px|link=|frameless|left]] | ||
| Line 141: | Line 143: | ||
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light. | If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light. | ||
</P> | </P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Solenopsis invicta'' 1 (BBa_K1415008)===== | =====PBAN ''Solenopsis invicta'' 1 (BBa_K1415008)===== | ||
[[File:SI.png|276px|link=|frameless|left]] | [[File:SI.png|276px|link=|frameless|left]] | ||
| Line 147: | Line 149: | ||
GSGEDLSYGDAYEVDEDDHPLFVPR | GSGEDLSYGDAYEVDEDDHPLFVPR | ||
<P>PBAN(SI)+pSB1C3 This biobrick is the PBAN of ''Solenopsis invicta'' on the pSB1C3 backbone.</P> | <P>PBAN(SI)+pSB1C3 This biobrick is the PBAN of ''Solenopsis invicta'' on the pSB1C3 backbone.</P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Solenopsis invicta'' 2 (BBa_K1415108)===== | =====PBAN ''Solenopsis invicta'' 2 (BBa_K1415108)===== | ||
[[File:HALFSI.png|457px|link=|frameless|left]] | [[File:HALFSI.png|457px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(SI) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(SI) | ||
| - | This circuit is responsible for producing the ''Solenopsis invicta''’s PBAN. As ''Solenopsis invicta''’s PBAN is ingested by the female ''Solenopsis invicta'', the female ''Solenopsis invicta'' will produce its own sex pheromone and attract male ''Solenopsis invicta''s.</P> | + | This circuit is responsible for '''producing the ''Solenopsis invicta''’s PBAN'''. As ''Solenopsis invicta''’s PBAN is ingested by the female ''Solenopsis invicta'', the female ''Solenopsis invicta'' will produce its own sex pheromone and attract male ''Solenopsis invicta''s.</P> |
| - | + | <h1></h1> | |
=====PBAN ''Solenopsis invicta'' 3 (BBa_K1415208)===== | =====PBAN ''Solenopsis invicta'' 3 (BBa_K1415208)===== | ||
[[File:ALLSI.png|780px|link=|frameless|left]] | [[File:ALLSI.png|780px|link=|frameless|left]] | ||
| Line 159: | Line 161: | ||
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light. | If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light. | ||
</P> | </P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Aedes aegypti'' 1 (BBa_K1415009)===== | =====PBAN ''Aedes aegypti'' 1 (BBa_K1415009)===== | ||
[[File:AA.png|276px|link=|frameless|left]] | [[File:AA.png|276px|link=|frameless|left]] | ||
| Line 165: | Line 167: | ||
DASSSNENNSRPPFAPRL | DASSSNENNSRPPFAPRL | ||
<P>PBAN(AA)+pSB1C3 This biobrick is the PBAN of ''Aedes aegypti'' on the pSB1C3 backbone.</P> | <P>PBAN(AA)+pSB1C3 This biobrick is the PBAN of ''Aedes aegypti'' on the pSB1C3 backbone.</P> | ||
| - | + | <h1></h1> | |
=====PBAN ''Aedes aegypti'' 2 (BBa_K1415109)===== | =====PBAN ''Aedes aegypti'' 2 (BBa_K1415109)===== | ||
[[File:HALFAA.png|457px|link=|frameless|left]] | [[File:HALFAA.png|457px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA) | ||
| - | This circuit is responsible for producing the ''Aedes aegypti''’s PBAN. As ''Aedes aegypti''’s PBAN is ingested by the female ''Aedes aegypti'', the female ''Aedes aegypti'' will produce its own sex pheromone and attract male ''Aedes aegyptis''.</P> | + | This circuit is responsible for '''producing the ''Aedes aegypti''’s PBAN'''. As ''Aedes aegypti''’s PBAN is ingested by the female ''Aedes aegypti'', the female ''Aedes aegypti'' will produce its own sex pheromone and attract male ''Aedes aegyptis''.</P> |
| - | + | <h1></h1> | |
=====PBAN ''Aedes aegypti'' 3 (BBa_K1415209)===== | =====PBAN ''Aedes aegypti'' 3 (BBa_K1415209)===== | ||
[[File:ALLAA.png|780px|link=|frameless|left]] | [[File:ALLAA.png|780px|link=|frameless|left]] | ||
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) | <P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) | ||
| - | The above circuit is constructed mainly to test whether it produces ''Aedes aegypti'' PBAN. | + | The above circuit is constructed mainly to test whether it produces ''Aedes aegypti''’s PBAN. |
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.</p> | If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.</p> | ||
| - | + | <h1></h1> | |
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 02:25, 18 October 2014
Parts Submitted to The Registry
Please click on the name of the parts for detailed information that is hosted in the Registry website. <groupparts>iGEM014 NCTU_Formosa</groupparts>
Brief Information
PBAN Bombyx mori 1 (BBa_K1415001)
Peptide Sequence: LSEDMPATPADQEMYQPDPEEMESRTRYFSPRL
PBAN(BM)+pSB1C3 This biobrick is the PBAN of Bombyx mori on the pSB1C3 backbone.
PBAN Bombyx mori 2 (BBa_K1415101)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(BM) This circuit is responsible for producing the Bombyx mori’s PBAN. As Bombyx mori PBAN is ingested by the female Bombyx mori, the female Bombyx mori will produce its own sex pheromone and attract male Bombyx moris.
PBAN Bombyx mori 3 (BBa_K1415201)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(BM) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Bombyx mori’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light.
PBAN Mamestra brassicae 1 (BBa_K1415002)
Peptide Sequence: LADDMPATPADQEMYRPDPEQIDSRTKYFSPRL
PBAN(MB)+pSB1C3 This biobrick is the PBAN of Mamestra brassicae on the pSB1C3 backbone.
PBAN Mamestra brassicae 2 (BBa_K1415102)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(MB) This circuit is responsible for producing the Mamestra brassicae’s PBAN. As Mamestra brassicae’s PBAN is ingested by the female Mamestra brassicae, the female Mamestra brassicae will produce its own sex pheromone and attract male Mamestra brassicaes.
PBAN Mamestra brassicae 3 (BBa_K1415202)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(MB) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Mamestra brassicae’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light.
PBAN Agrotis ipsilon 1 (BBa_K1415003)
Peptide Sequence: LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL
PBAN(AI)+pSB1C3 This biobrick is the PBAN of Agrotis ipsilon on the pSB1C3 backbone.
PBAN Agrotis ipsilon 2 (BBa_K1415103)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AI) This circuit is responsible for producing the Agrotis ipsilon’s PBAN. As Agrotis ipsilon’s PBAN is ingested by the female Agrotis ipsilon, the female Agrotis ipsilon will produce its own sex pheromone and attract male Agrotis ipsilons.
PBAN Agrotis ipsilon 3 (BBa_K1415203)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AI) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Agrotis ipsilon’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light.
PBAN Lymantria dispar 1 (BBa_K1415004)
Peptide Sequence: LADDMPATMADQEVYRPEPEQIDSRNKUFSPRL
PBAN(AI)+pSB1C3 This biobrick is the PBAN of Lymantria dispar on the pSB1C3 backbone.
PBAN Lymantria dispar 2 (BBa_K1415104)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(LD) This circuit is responsible for producing Lymantria dispar’s PBAN. As Lymantria dispar PBAN is ingested by the female Lymantria dispar, the female Lymantria dispar will produce its own sex pheromone and attract male Lymantria dispars.
PBAN Lymantria dispar 3 (BBa_K1415204)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(LD) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Lymantria dispar’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
PBAN Spodoptera litura 1 (BBa_K1415005)
Peptide Sequence: LADDMPATPADQELYRPDPDQIDSRTKUFSPRL
PBAN(SL)+pSB1C3 This biobrick is the PBAN of Spodoptera litura on the pSB1C3 backbone.
PBAN Spodoptera litura 2 (BBa_K1415105)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(SL) This circuit is responsible for producing the Spodoptera litura’s PBAN. As Spodoptera litura PBAN is ingested by the female Spodoptera litura, the female Spodoptera litura will produce its own sex pheromone and attract male Spodoptera lituras.
PBAN Spodoptera litura 3 (BBa_K1415205)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(SL) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Spodoptera litura’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
PBAN Helicoverpa armigera Hubner 1 (BBa_K1415006)
Peptide Sequence: LSDDMPARPADQEMYRQDPEQIDSRTKYFSPRL
PBAN(HAH)+pSB1C3 This biobrick is the PBAN of Helicoverpa armigera Hubner on the pSB1C3 backbone.
PBAN Helicoverpa armigera Hubner 2 (BBa_K1415106)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(HAH) This circuit is responsible for producing the Helicoverpa armigera Hubner’s PBAN. As Helicoverpa armigera Hubner’s PBAN is ingested by the female Helicoverpa armigera Hubner, the female Helicoverpa armigera Hubner will produce its own sex pheromone and attract male Helicoverpa armigera Hubners.
PBAN Helicoverpa armigera Hubner 3 (BBa_K1415206)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(HAH) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Helicoverpa armigera Hubner’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
PBAN Adoxophyes sp. 1 (BBa_K1415007)
Peptide Sequence: QSEAVTSSDEQVYRQDMSPVDGRLKYFSPRL
PBAN(AS)+pSB1C3 This biobrick is the PBAN of Adoxophyes sp. on the pSB1C3 backbone.
PBAN Adoxophyes sp. 2 (BBa_K1415107)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AS) This circuit is responsible for producing the Adoxophyes sp.’s PBAN. As Adoxophyes sp.’s PBAN is ingested by the female Adoxophyes sp., the female Adoxophyes sp. will produce its own sex pheromone and attract male Adoxophyes sp.
PBAN Adoxophyes sp. 3 (BBa_K1415207)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AS) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Adoxophyes sp.’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
PBAN Solenopsis invicta 1 (BBa_K1415008)
Peptide Sequence: GSGEDLSYGDAYEVDEDDHPLFVPR
PBAN(SI)+pSB1C3 This biobrick is the PBAN of Solenopsis invicta on the pSB1C3 backbone.
PBAN Solenopsis invicta 2 (BBa_K1415108)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(SI) This circuit is responsible for producing the Solenopsis invicta’s PBAN. As Solenopsis invicta’s PBAN is ingested by the female Solenopsis invicta, the female Solenopsis invicta will produce its own sex pheromone and attract male Solenopsis invictas.
PBAN Solenopsis invicta 3 (BBa_K1415208)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(SI) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Solenopsis invicta PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
PBAN Aedes aegypti 1 (BBa_K1415009)
Peptide Sequence: DASSSNENNSRPPFAPRL
PBAN(AA)+pSB1C3 This biobrick is the PBAN of Aedes aegypti on the pSB1C3 backbone.
PBAN Aedes aegypti 2 (BBa_K1415109)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA) This circuit is responsible for producing the Aedes aegypti’s PBAN. As Aedes aegypti’s PBAN is ingested by the female Aedes aegypti, the female Aedes aegypti will produce its own sex pheromone and attract male Aedes aegyptis.
PBAN Aedes aegypti 3 (BBa_K1415209)
Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Aedes aegypti’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
