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Team:Hannover/Protocols/Cloning/Ligation
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| - | <h1><a href="https://2014.igem.org/Team:Hannover/Protocols | + | <h1><a href="https://2014.igem.org/Team:Hannover/Protocols">Protocols</a> / Ligation </h1> |
<p class="text">After restriction enzyme cleavage, compatible ends of two fragments can hybridize, but are not stable due to their missing phosphodiester bonds. To fix those nicks in the double-stranded DNA, a ligase from T4 phages (Thermo Scientific) was utilized. The ligation reaction mix is shown in the following table. </p> | <p class="text">After restriction enzyme cleavage, compatible ends of two fragments can hybridize, but are not stable due to their missing phosphodiester bonds. To fix those nicks in the double-stranded DNA, a ligase from T4 phages (Thermo Scientific) was utilized. The ligation reaction mix is shown in the following table. </p> | ||
Latest revision as of 23:35, 17 October 2014
Protocols / Ligation
After restriction enzyme cleavage, compatible ends of two fragments can hybridize, but are not stable due to their missing phosphodiester bonds. To fix those nicks in the double-stranded DNA, a ligase from T4 phages (Thermo Scientific) was utilized. The ligation reaction mix is shown in the following table.
Table 1: Reaction Mixes and Temperature Programs for the Ligation.
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