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Team:Evry/Notebook/Transposons/08.06.14
From 2014.igem.org
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It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon. | It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon. | ||
<br/> A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein. | <br/> A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein. | ||
| - | <br/>It parallel annotation of plasmid pNK2 with Blast is realized.<br/> | + | <br/>It parallel annotation of plasmid pNK2 with Blast is realized.<br/></p> |
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<img src="https://static.igem.org/mediawiki/2014/6/64/PNK2.png" width="50%"/><br/> | <img src="https://static.igem.org/mediawiki/2014/6/64/PNK2.png" width="50%"/><br/> | ||
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<span class="cd-date">Aug 08</span> | <span class="cd-date">Aug 08</span> | ||
Latest revision as of 20:06, 17 October 2014
It’s the first day where Brian Jester gave us the plasmid pNK2. This plasmid contains transposon.
A Precultur of DH5α pir is realized.In a 15 mL tube, add 2 mL of LB medium and inoculate cells from glycerol. These cells can replicate the plasmid because they contains pi protein.
It parallel annotation of plasmid pNK2 with Blast is realized.
