Team:Groningen/Template/MODULE/Notebook/detection/week1
From 2014.igem.org
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- | The following BioBricks were transformed to <i>E. coli</i> DH5α | + | The following BioBricks were taken from the 2014 iGEM distribution kit, and transformed to <i>E. coli</i> DH5α |
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- | <div class="item"><a href="http://parts.igem.org/Part:BBa_ B0015">BBa_ B0015</a></div> | + | <div class="item">The double terminator(<a href="http://parts.igem.org/Part:BBa_ B0015">BBa_ B0015</a>)</div> |
- | <div class="item"><a href="http://parts.igem.org/Part:BBa_ B0034">BBa_ B0034</a></div> | + | <div class="item">The RBS(<a href="http://parts.igem.org/Part:BBa_ B0034">BBa_ B0034</a>)</div> |
- | <div class="item"><a href="http://parts.igem.org/Part:BBa_ K592025">BBa_ K592025</a></div> | + | <div class="item">The gene for the purple-blue chromoprotein amilCP(<a href="http://parts.igem.org/Part:BBa_ K592025">BBa_ K592025</a>)</div> |
- | <div class="item"><a href="http://parts.igem.org/Part:BBa_ I746101">BBa_ I746101</a></div> | + | <div class="item">The genes for the AIP sensor infrastructure(<a href="http://parts.igem.org/Part:BBa_ I746101">BBa_ I746101</a>)</div> |
- | <div class="item"><a href="http://parts.igem.org/Part:BBa_ E0040">BBa_ E0040</a></div> | + | <div class="item">The gene coding for GFP(<a href="http://parts.igem.org/Part:BBa_ E0040">BBa_ E0040</a>)</div> |
- | <div class="item"><a href="http://parts.igem.org/Part:BBa_ S03156">BBa_ S03156</a></div> | + | <div class="item">The <i>lasR</i> gene(<a href="http://parts.igem.org/Part:BBa_ S03156">BBa_ S03156</a>)</div> |
- | <div class="item"><a href="http://parts.igem.org/Part:BBa_ R0079">BBa_ R0079</a></div> | + | <div class="item">The promoter LasR(<a href="http://parts.igem.org/Part:BBa_ R0079">BBa_ R0079</a>)</div> |
- | <div class="item"><a href="http://parts.igem.org/Part:BBa_ K081009">BBa_ K081009</a></div> | + | <div class="item">The <i>lasI</i> protein generator(<a href="http://parts.igem.org/Part:BBa_ K081009">BBa_ K081009</a>)</div> |
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+ | From all transformations a glycerol stock was made, and stored at - 80 °C | ||
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Latest revision as of 19:39, 17 October 2014
July 28 - August 3
The following BioBricks were taken from the 2014 iGEM distribution kit, and transformed to E. coli DH5α
The double terminator(BBa_ B0015)
The RBS(BBa_ B0034)
The gene for the purple-blue chromoprotein amilCP(BBa_ K592025)
The genes for the AIP sensor infrastructure(BBa_ I746101)
The gene coding for GFP(BBa_ E0040)
The lasR gene(BBa_ S03156)
The promoter LasR(BBa_ R0079)
The lasI protein generator(BBa_ K081009)
All samples were digested, and checked on gel
From all transformations a glycerol stock was made, and stored at - 80 °C