Team:Nankai

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<!--Thanks to Nelson's works.make Bootstrap template (any outside servers) perfectly work in iGEM wiki system. How to? First you buy a server and upload your websites, and then copy my code below, replace your servers address. Second, in every iGEM wiki page, put the similar code.(How to edit page? Replace your school name (and year ) in "https://2014.igem.org/wiki/index.php?title=Team:Nankai&action=edit" and put it in your browser) (and if you want to edit Nankai/Team, just add /Team in that address) Third, in every linkage, replace "href="xxx" by "onclick=window.parent.location.href="xxx"", if you have pdf file, please upload to iGEM and paste the address. If you have further question please feel free to ask Nelson's email: yuiuiuiuiui@163.com-->
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WEBSITE NAME HERE</td>
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<td height="36" colspan="3" id="navigation" class="navText"><a href="javascript:;">HOME</a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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      <a href="javascript:;">PROJECT</a>&nbsp;&nbsp;&nbsp;&nbsp; <a href="javascript:;"></a>&nbsp;PHYSICAL MODELING &nbsp;
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      &nbsp;&nbsp;&nbsp;PARTS<a href="javascript:;"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; PRACTICES &nbsp;&nbsp;&nbsp;&nbsp; TEAM </td>
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  <span class="STYLE9">&nbsp;<span class="STYLE2">Abstract</span></span><br /> </td>
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  <span class="STYLE1">Microbial enhanced oil recovery (MEOR) is a popular method that relies on microorganisms and their metabolic products to mobilize residual oil. Rhamnolipid, a widely-used biosurfactant produced by <em>Pseudomonas aeruginosa</em> under aerobic conditions, is helpful in reducing oil-water interfacial tension, which makes it easier to remove oil. However, because of the anaerobic environment in oil reservoirs, rhamnolipid has to be produced ex situ, and then injected into the oil reservoirs. Our project is to build a bacterial strain that is able to utilize oil as its carbon resource as well as producing rhamnolipid in anaerobic environment. We start with a <em>Pseudomonas stutzeri</em> strain that lives in the oil reservoirs, clone and make it express the rhamnosyltransferase operon genes rhlABRI from Pseudomonas aeruginosa so that it can produce and excrete rhamnolipid into the oil constantly. With all the strengths enhancing the oil recovery, we name our engineered bacterium &quot;Super Petroleum Magician&quot;.</span></td>
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      <td width="470" colspan="9"><span class="STYLE1">Microbial enhanced oil recovery (MEOR) is a popular method today in oil production that relies on microorganisms and their metabolic products to mobilize residual oil. Early in 1946 people began to study the application of biosurfactants in oil production, when Zobell discovered that microbe-producing biosurfactants are one of the mechanisms of MEOR. Biosurfactants lower the oil-water interfacial tension, letting the oil droplets release from the sandstones surface, and reduce the viscosity of heavy oil to help its flow. The production of biosurfactants in aerobic environment has been comprehensively researched. Rhamnolipid for instance, has been extensively studied and applied because of its remarkabl surface activity and emulsifying ability.</span></td>
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<p><span class="STYLE13">Biosurfactants in MEOR</span></p>
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<p class="STYLE1">Until today, it remains at a stage where we have to produce our biosurfactant like rhamnolipid at a bioreactor and then injected them into the oil reservoir. The process of product purification and transportation is very costly and complex, which makes it too unpractical to employ in offshore oil wells. In the meantime, the chance for biosurfactants molecules to meet residual oil along the water pathway is very small. We can’t target the oil droplets separated all over the oil field which is often half wrapped by sandstones. Thus the increase of oil output depends largely on the amount of biosurfactants we pump into the well, causing its low efficiency.</p>
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Latest revision as of 01:30, 18 October 2014

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