Team:TCU Taiwan/Notebook

*LB(Luria-Bertani) broth: 1% Trytone, 1% NaCl, 0.5% Yeast extract in the ddH2O
*LB agar plate:LB broth add 1.5% agar.If you want to add selection marker example for ampicillin or chloramphenicol. To culture E.coli, ampicillin is added 100 mg/ml for usage, chloramphenicol is added 30 mg/ml for usage, kanamycin is added 70 mg/ml for usage

*Solution I: 50 mM glucose、25 mM Tris-HCl (pH 8.0)、10 mM EDTA
*Solution II:0.2N NaOH and 1% SDS(Sodium dodecyl sulfate ) mix
*Solution III: 5 M potassium acetate (pH4.8), 11.5% glacial acetic acid
*RNase A(10 mg/ml)
*Phenol chloroform
*Chloroform
*95% Ethanol
*Tris-EDTA(TE)buffer:10 mM Tris-Cl(pH 7.5) 1 mM EDTA
*pBluescript SK(-)
*BBa_K1218011
*BBa_K914003
*BBa_I13521

*Quick screening buffer(Tris-Cl pH 12.6, 3% SDS)
*Phenol chloroform
*Chloroform

*T4 ligase
*10X ligation buffer
*EcoRI
*PstI
*SpeI
*XbaI
*BSA 0.1%

*DH5a
*JM101
*0.8% and 2% agarose gel
*95% Ethanol
*6X loading dye
*1.5 ml centrifuge tube
*250 ml flask
*M13KO7(1*1011 pfu/ml)from NEW ENGLAND BioLabs

*Top DNA polymerase
*dNTP(dATP, dCTP, dGTP, dTTP) 250 mM
*Tris-HCl(pH9.0) 10 mM
*KCl 30 mM
*MgCl2 1.5 mM

*VCM-620, laminal flow
*Biofuge pico, Heraeus
* OS1500-H, TSK Oribital Shaking Iucubator
* Biowave
* Vortex-GENE 2
* Autoclave AS-1060L
* GenAmp PCR System 2400, Applied Biosystems
*BU-420, YIHDER
*5804R, eppendorf Centrifuge