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Team:SYSU-China/file/Project/notebook/lab note/mutation
From 2014.igem.org
(Difference between revisions)
(Created page with "May: amplify biobricks June: clone the WT DNAQ ,recA,umuC,umuD from E.coli July: perform the point-mutation and truncation of the four genes August: add prefix and suffix to th...") |
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| - | May: amplify biobricks | + | <p>May: amplify biobricks </p> |
| - | June: clone the WT DNAQ ,recA,umuC,umuD from E.coli | + | <p>June: clone the WT DNAQ ,recA,umuC,umuD from E.coli </p> |
| - | July: perform the point-mutation and truncation of the four genes | + | <p>July: perform the point-mutation and truncation of the four genes</p> |
| - | August: add prefix and suffix to the genes | + | <p>August: add prefix and suffix to the genes </p> |
| - | + | <p>Add RBS to the genes</p> | |
| - | September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;) | + | <p>September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;)</p> |
| - | + | <p>Add promoter to construct the expression vector</p> | |
| - | + | <p> Test the mutation rate</p> | |
| - | October: adapt the testing method and test the mutation rates | + | <p>October: adapt the testing method and test the mutation rates</p> |
| - | + | <p>Prepare the biobrick for submission </p> | |
Latest revision as of 18:18, 17 October 2014
May: amplify biobricks
June: clone the WT DNAQ ,recA,umuC,umuD from E.coli
July: perform the point-mutation and truncation of the four genes
August: add prefix and suffix to the genes
Add RBS to the genes
September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;)
Add promoter to construct the expression vector
Test the mutation rate
October: adapt the testing method and test the mutation rates
Prepare the biobrick for submission
