Team:Cooper Union/Notebook/Biohack May

From 2014.igem.org

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<ul class="menu">
<ul class="menu">
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan</a> </li>
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan Timer</a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
 +
<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
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<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
 
</ul>
</ul>
</li>
</li>
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<li class="menu-safety"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li>
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<li class="menu-safety"> Social
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<li class="menu-outreach"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
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<ul class="menu">
 +
<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li>
 +
<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Ethics">Ethics</a> </li>
 +
<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
 +
</ul>
 +
</li>
<li class="menu-notebook">  Notebook  
<li class="menu-notebook">  Notebook  
<ul class="menu">
<ul class="menu">
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     <br style="clear:both" />
     <br style="clear:both" />
</div>
</div>
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<pre>
 
-
5/19/14
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<h3>5/19/14</h3>
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Calibrated Nanodrop.
+
<ul><li>Calibrated Nanodrop.</li>
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Nanodropped all of our samples. Very low concentration of DNA.
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<li>Nanodropped all of our samples. Very low concentration of DNA.</li>
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Ordered primers to PCR our g-blocks.
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<li>Ordered primers to PCR our g-blocks.</li>
 +
</ul>
-
5/22/14:
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<h3>5/22/14</h3>
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Struck out plates with pACYC184 with spectinomycin to grow it.
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<ul>
-
Digested pBR322 with HindIII.
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  <li>Struck out plates with pACYC184 with spectinomycin to grow it.</li>
-
Double digested pBR322 with HindIII/EcoRI.
+
  <li>Digested pBR322 with HindIII.</li>
 +
  <li>Double digested pBR322 with HindIII/EcoRI.</li>
 +
</ul>
-
5/23/14
+
<h3>5/23/14</h3>
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Ran gel of digests from 5/22/14. Everything as it should be.
+
<ul>
-
Purified the digested plasmids.  
+
  <li>Ran gel of digests from 5/22/14. Everything as it should be.</li>
-
Nanodropped plasmids; results:
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  <li>Purified the digested plasmids. </li>
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Single digest I: 52.7ng/uL
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  <li>Nanodropped plasmids; results:
-
Single digest II: 64.5ng/uL
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<dl><dd>Single digest I: 52.7ng/&mu;L</dd>
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Double digest I: 26.3ng/uL
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<dd>Single digest II: 64.5ng/&mu;L</dd>
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Double digest II: 41.9ng/uL
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<dd>Double digest I: 26.3ng/&mu;L</dd>
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Phosphatase treated the four digested plasmids.  
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<dd>Double digest II: 41.9ng/&mu;L</dd>
 +
</dl>
 +
</li>
 +
  <li>Phosphatase treated the four digested plasmids. </li>
 +
</ul>
-
5/27/14
 
-
Purified phosphatase treated plasmids from 5/23/14.
 
-
Nanodropped the purified plasmids; results:
 
-
Single digest I: 43.4ng/uL
 
-
Single digest II: 53.6ng/uL
 
-
Double digest I: 43.7ng/uL
 
-
Double digest II: 16.5ng/uL
 
-
</pre>  
+
<h3>5/27/14</h3>
 +
<ul>
 +
  <li>Purified phosphatase treated plasmids from 5/23/14. </li>
 +
  <li>Nanodropped the purified plasmids; results:
 +
<dl><dd>Single digest I: 43.4ng/&mu;L</dd>
 +
<dd>Single digest II: 53.6ng/&mu;L</dd>
 +
<dd>Double digest I: 43.7ng/&mu;L</dd>
 +
<dd>Double digest II: 16.5ng/&mu;L</dd>
 +
</dl></li>
 +
</ul>
 +
<h3>5/28/14</h3>
<h3>5/28/14</h3>
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<img src=" https://static.igem.org/mediawiki/2014/0/06/CU_528_Gel.jpg " width="200" />
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<img src=" https://static.igem.org/mediawiki/2014/0/06/CU_528_Gel.jpg " width="350" />
-
 
+
-
 
+
-
</pre>
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             <span>Projects</span>
             <span>Projects</span>
         </a>
         </a>
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         <a href="https://2014.igem.org/Team:Cooper_Union/Safety" class="red" >
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         <a href="https://2014.igem.org/Team:Cooper_Union/Social" class="red" >
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             <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" />
+
             <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Social" />
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             <span>Safety</span>
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             <span>Social</span>
-
        </a>
+
-
        <a href="https://2014.igem.org/Team:Cooper_Union/Outreach"  class="yellow">
+
-
            <img src="https://static.igem.org/mediawiki/2014/7/74/CU_2014_logoO.png" height="100" style="vertical-align: top;" title="Outreach" />
+
-
            <span>Outreach</span>
+
         </a>
         </a>
         <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >
         <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >

Latest revision as of 00:45, 18 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


5/19/14

  • Calibrated Nanodrop.
  • Nanodropped all of our samples. Very low concentration of DNA.
  • Ordered primers to PCR our g-blocks.

5/22/14

  • Struck out plates with pACYC184 with spectinomycin to grow it.
  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII/EcoRI.

5/23/14

  • Ran gel of digests from 5/22/14. Everything as it should be.
  • Purified the digested plasmids.
  • Nanodropped plasmids; results:
    Single digest I: 52.7ng/μL
    Single digest II: 64.5ng/μL
    Double digest I: 26.3ng/μL
    Double digest II: 41.9ng/μL
  • Phosphatase treated the four digested plasmids.

5/27/14

  • Purified phosphatase treated plasmids from 5/23/14.
  • Nanodropped the purified plasmids; results:
    Single digest I: 43.4ng/μL
    Single digest II: 53.6ng/μL
    Double digest I: 43.7ng/μL
    Double digest II: 16.5ng/μL

5/28/14



Projects Social Notebook Team

Retrieved from "http://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_May"