Team:Cooper Union/Notebook/Biohack April

From 2014.igem.org

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<ul class="menu">
<ul class="menu">
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan</a> </li>
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<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan Timer</a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
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<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
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<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
 
</ul>
</ul>
</li>
</li>
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<li class="menu-safety"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li>
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<li class="menu-safety"> Social
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<li class="menu-outreach"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
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<ul class="menu">
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<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li>
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<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Ethics">Ethics</a> </li>
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<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
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</ul>
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</li>
<li class="menu-notebook">  Notebook  
<li class="menu-notebook">  Notebook  
<ul class="menu">
<ul class="menu">
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             <span>Projects</span>
             <span>Projects</span>
         </a>
         </a>
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         <a href="https://2014.igem.org/Team:Cooper_Union/Safety" class="red" >
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         <a href="https://2014.igem.org/Team:Cooper_Union/Social" class="red" >
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             <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" />
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             <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Social" />
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             <span>Safety</span>
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             <span>Social</span>
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        </a>
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        <a href="https://2014.igem.org/Team:Cooper_Union/Outreach"  class="yellow">
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            <img src="https://static.igem.org/mediawiki/2014/7/74/CU_2014_logoO.png" height="100" style="vertical-align: top;" title="Outreach" />
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            <span>Outreach</span>
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         </a>
         </a>
         <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >
         <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >

Latest revision as of 00:44, 18 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


3/13/14

  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII and EcoRI.

3/27/14

  • Ran digests from 3/13/14 on gel.
  • Excised gel fragments with plasmid in them.
  • Purified gel fragments.

4/10/14

Phosphatase treated the digested and double digested pBR322 from 3/13/14. 4/17/14
  • Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI.
  • Performed five ligations:
    pBR322 with UV
    pBR322 with HSP
    pBR322 with GFP
    UV with GFP
    HSP with GFP

4/24/14

  • Purified UV+GFP and HSP+GFP ligations.
  • Digested the two ligations with HindIII.
  • Ligated each one into pBR322.


Projects Social Notebook Team

Retrieved from "http://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_April"