Team:UT-Tokyo/Counter/Project/Lab

(Difference between revisions)

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Contents 1 Favorite Parts 2 Other Pars 3 DNA construction 3.1 Transformation 3.2 Electrophoresis 3.3 Colony PCR 3.4 Miniprep 3.5 Digestion 3.6 Gel extraction 3.7 Ligation 4 Assay method 4.1 Influence on growth of E. coli 4.2 Sigma factor and its crresponding promoter 4.3 Reset function 4.4 Riboregulator 4.5 Positive feedback 5 7,14,2014 5.1 Lab member 5.2 Contents 5.2.1 Making Plate Culture 6 7,15,2014 6.1 Lab member 6.2 Contents 6.2.1 Making DW 100mL 6.2.2 TF 7 7,16,2014 7.1 Lab member 7.2 Contents 7.2.1 TF 7.2.2 preculture 8 7,17,2014 8.1 Lab member 8.2 Contents 8.2.1 Making glycerol stock/miniprep 8.2.2 making reagent 8.2.3 preculture 9 7,18,2014 9.1 Lab member 9.2 Contents 9.2.1 miniprep 9.2.2 Making competent cell 9.2.3 making reagent 10 7,22,2014 10.1 Lab member 10.2 Contents 10.2.1 Cut check 10.2.2 TF 10.2.3 TF 10.2.4 preculture 11 7,23,2014 11.1 Lab member 11.2 Contents 11.2.1 miniprep 11.2.2 Making plate 12 7,28,2014 12.1 Lab member 12.2 Contents 12.2.1 digestion,gel extraction 12.2.2 colony PCR 12.2.3 Measuring cfu 13 7,29,2014 13.1 Lab member 13.2 Contents 13.2.1 TF 13.2.2 Measuring cfu 13.2.3 Electrophoresis check 14 7,30,2014 14.1 Lab member 14.2 Contents 14.2.1 colony check 14.2.2 Cut check 14.2.3 colony PCR 14.2.4 preculture 15 7,31,2014 15.1 Lab member 15.2 Contents 15.2.1 miniprep 15.2.2 digestion,gel extraction 15.2.3 ligation 16 8,1,2014 16.1 Lab member 16.2 Contents 16.2.1 TF 16.2.2 ligation Check 17 8,4,2014 17.1 Lab member 17.2 Contents 17.2.1 Plate check 17.2.2 digestion 17.2.3 Electrophoresis 17.2.4 preculture 18 8,5,2014 18.1 Lab member 18.2 Contents 18.2.1 miniprep 18.2.2 Cut check 18.2.3 Elrctrophoresis 18.2.4 preculture 19 8,6,2014 19.1 Lab member 19.2 Contents 19.2.1 miniprep 19.2.2 digestion, gel extraction 19.2.3 making gel 19.2.4 ligation 19.2.5 TF 20 8，7,2014 20.1 Lab member 20.2 Contents 20.2.1 colony PCR 20.2.2 digestion, gel extraction 20.2.3 ligation 20.2.4 Cut check 21 8,8,2014 21.1 Lab member 21.2 Contents 21.2.1 Cut check sample Electrophoresis 21.2.2 digestion, gel extraction 21.2.3 ligation 21.2.4 TF 21.2.5 overlap extension PCR →PCR clean up 21.2.6 making gel 22 8,9,2014 22.1 Lab member 22.2 Contents 22.2.1 Plate and test tube check 23 8,11,2014 23.1 Lab member 23.2 Contents 23.2.1 colony PCR 23.2.2 overlap extension PCR →PCR clean up 23.2.3 Making Plate 23.2.4 preculture 24 8,12,2014 24.1 Lab member 24.2 Contents 24.2.1 miniprep 24.2.2 digestion →gel extraction 24.2.3 colony PCR 24.2.4 ligation →TF 24.2.5 Making　reagent 25 8,13,2014 25.1 Lab member 25.2 Contents 25.2.1 miniprep 25.2.2 digestion→ gel extrction 25.2.3 ligation→TF 25.2.4 colony PCR 25.2.5 preculture 26 8,14,2014 26.1 Lab member 26.2 Contents 26.2.1 miniprep 26.2.2 overlap extension PCR 26.2.3 digestion → gel extranction 26.2.4 gel making 26.2.5 colony PCR 26.2.6 ligation → TF 27 8,15,2014 27.1 Lab member 27.2 Contents 27.2.1 miniprep 27.2.2 digestion → gel extraction 27.2.3 colony PCR 27.2.4 ligation → TF 27.2.5 preculture 28 8,16,2014 28.1 Lab member 28.2 Contents 28.2.1 miniprep 29 8,18,2014 29.1 Lab member 29.2 Contents 29.2.1 digestion → gel extraction 29.2.2 sequence 29.2.3 colony PCR 29.2.4 ligation → TF 29.2.5 preculture 30 8,19,2014 30.1 Lab member 30.2 Contents 30.2.1 miniprep 30.2.2 digestion 30.2.3 making gel 30.2.4 colony PCR 30.2.5 sequence 31 8,20,2014 31.1 Lab member 31.2 Contents 31.2.1 PCR 31.2.2 TF 32 8,21,2014 32.1 Lab member 32.2 Contents 32.2.1 digestion → gel extraction 32.2.2 colony PCR 32.2.3 ligation → TF 32.2.4 preculture 33 8,22,2014 33.1 Lab member 33.2 Contents 33.2.1 miniprep 33.2.2 colony PCR 33.2.3 digestion → gel extraction 33.2.4 ligation → TF 33.2.5 preculture 34 8,23,2014 34.1 Lab member 34.2 Contents 34.2.1 miniprep 35 8,25,2014 35.1 Lab member 35.2 Contents 35.2.1 digestion → gel extraction 35.2.2 Plate　making 35.2.3 colony PCR 35.2.4 sequence 35.2.5 ligation → TF 35.2.6 preculture 36 8,26,2014 36.1 Lab member 36.2 Contents 36.2.1 miniprep 36.2.2 colony PCR 36.2.3 digestion → gel extraction 36.2.4 ligation → TF 36.2.5 preculture 36.2.6 sequence 36.2.7 sequence 37 8,27,2014 37.1 Lab member 37.2 Contents 37.2.1 miniprep 37.2.2 digestion → gel extraction 37.2.3 colony PCR 37.2.4 ligation → TF 37.2.5 preculture 37.2.6 Remarks 38 8,28,2014 38.1 Lab member 38.2 Contents 38.2.1 miniprep 38.2.2 digestion →gel extraction 38.2.3 overlap extension PCR 38.2.4 check & colony PCR 38.2.5 ligation → TF 39 8,29,2014 39.1 Lab member 39.2 Contents 39.2.1 miniprep 39.2.2 digestion →gel extraction 39.2.3 PCR 39.2.4 check & colony PCR 39.2.5 sequence order 39.2.6 ligation → TF 39.2.7 preculture 40 8,30,2014 40.1 Lab member 40.2 Contents 40.2.1 miniprep 40.2.2 PCR 41 9,1,2014 41.1 Lab member 41.2 Contents 41.2.1 PCR clean up 41.2.2 digestion → gel extraction 41.2.3 Making plate 41.2.4 colony PCR 41.2.5 Ligation 41.2.6 preculture 42 9,2,2014 42.1 Lab member 42.2 Contents 42.2.1 miniprep 42.2.2 digestion → gel extraction 42.2.3 sequence 42.2.4 PCR 42.2.5 check & colony PCR 42.2.6 ligation → TF 42.2.7 preculture 42.2.8 We got a result!! 43 9,3,2014 43.1 Lab member 43.2 Contents 43.2.1 sequence 43.2.2 digestion → gel extraction 43.2.3 colony PCR 43.2.4 PCR 43.2.5 ligation → TF 44 9,4,2014 44.1 Lab member 44.2 Contents 44.2.1 miniprep 44.2.2 digestion → gel extraction 44.2.3 PCR check & clean up 44.2.4 Making Plate 44.2.5 digestion 44.2.6 colony PCR 44.2.7 ligation → TF 44.2.8 miniprep 44.2.9 preculture 45 9,5,2014 45.1 Lab member 45.2 Contents 45.2.1 miniprep 45.2.2 digestion → gel extraction 45.2.3 ligation → TF 45.2.4 PCR 45.2.5 colony PCR 45.2.6 preculture 46 9,6,2014 46.1 Lab member 46.2 Contents 46.2.1 miniprep 46.2.2 PCR 46.2.3 check & colony PCR 47 9,8,2014 47.1 Lab member 47.2 Contents 47.2.1 digestion → gel extaction 47.2.2 colony PCR 47.2.3 PCR 47.2.4 ligation → TF 47.2.5 preculture 48 9,9,2014 48.1 Lab member 48.2 Contents 48.2.1 miniprep 48.2.2 digestion → gel extraction 48.2.3 colony PCR 48.2.4 sequence 48.2.5 ligation → TF 48.2.6 preculture 49 9,10,2014 49.1 Lab member 49.2 Contents 49.2.1 miniprep 49.2.2 digestion → gel extraction 49.2.3 colony PCR 49.2.4 ligation → TF 49.2.5 preculture 50 9,11,2014 50.1 Lab member 50.2 Contents 50.2.1 miniprep 50.2.2 digestion → gel extraction 50.2.3 sequence 50.2.4 Plate Making 50.2.5 colony PCR 50.2.6 ligation → TF 50.2.7 preculture 51 9,12,2014 51.1 Lab member 51.2 Contents 51.2.1 miniprep 51.2.2 digestion → gel extraction 51.2.3 colony PCR 51.2.4 sequence 51.2.5 ligation → TF 51.2.6 ligation 51.2.7 preculture 52 9,13,2014 52.1 Lab member 52.2 Contents 52.2.1 miniprep 53 9,15,2014 53.1 Lab member 53.2 Contents 53.2.1 digestion → gel extraction 53.2.2 colony PCR 53.2.3 ligation → TF 53.2.4 preculture 54 9,16,2014 54.1 Lab member 54.2 Contents 54.2.1 miniprep 54.2.2 colony PCR 54.2.3 digestion → gel extraction 54.2.4 ligation → TF 54.2.5 preculture 54.2.6 assay 55 9,17,2014 55.1 Lab member 55.2 Contents 55.2.1 miniprep 55.2.2 digestion → gel extraction 55.2.3 colony PCR 55.2.4 ligation → TF 55.2.5 assay 56 9,18,2014 56.1 Lab member 56.2 Contents 56.2.1 miniprep 56.2.2 digestion → gel extraction 56.2.3 colony PCR 56.2.4 ligation → TF 56.2.5 preculture 57 9,19,2014 57.1 Lab member 57.2 Contents 57.2.1 miniprep 57.2.2 digestion → gel extraction 57.2.3 colony PCR 57.2.4 ligation → TF 57.2.5 preculture 58 9,20,2014 58.1 Lab member 58.2 Contents 58.2.1 miniprep 59 9,22,2014 59.1 Lab member 59.2 Contents 59.2.1 digestion → gel extraction 59.2.2 colony PCR 59.2.3 ligation - TF 60 9,23,2014 60.1 Lab member 60.2 Contents 60.2.1 digestion, gel extraction 60.2.2 miniprep 60.2.3 colony PCR 60.2.4 ligation,TF 61 9,24,2014 61.1 Lab member 61.2 Contents 61.2.1 miniprep 61.2.2 digestion,gel extraction 61.2.3 ligation,TF 62 9,25,2014 62.1 Lab member 62.2 Contents 62.2.1 miniprep 62.2.2 digestion,gel extraction 62.2.3 ligation,TF 62.2.4 Making competent cell 62.2.5 preculture 62.2.6 competent cell(made at 0911) check 62.2.7 result of check 63 9,26,2014 63.1 Lab member 63.2 Contents 63.2.1 miniprep 63.2.2 digestion 63.2.3 colony PCR 63.2.4 ligation,TF 63.2.5 preculture 64 9,27,2014 64.1 Lab member 64.2 Contents 64.2.1 TF 64.2.2 miniprep 64.2.3 TF 65 9,29,2014 65.1 Lab member 65.2 Contents 65.2.1 digestion,gel extraction 65.2.2 Gel making 65.2.3 ligation 65.2.4 preculture 65.2.5 PCR 66 9,30,2014 66.1 Lab member 66.2 Contents 66.2.1 miniprep 66.2.2 digestion,gel extraction 66.2.3 colony PCR 66.2.4 Making M9 medium 66.2.5 digestion(cutcheck) 67 10,1,2014 67.1 Lab member 67.2 Contens 67.2.1 miniprep 67.2.2 sequence order 68 10,2,2014 68.1 Lab member 68.2 Contents 68.2.1 assay 68.2.2 culture 68.2.3 PCR 68.2.4 Making M9 medium 68.2.5 PCR check 68.2.6 PCR 68.2.7 preculture 69 10,3,2014 69.1 Lab member 69.2 Contents 69.2.1 PCR clean up and check 69.2.2 digestion,gel extraction 69.2.3 PCR 69.2.4 gel making 69.2.5 ligation,TF 69.2.6 PCR 70 10,4,2014 70.1 Lab member 70.1.1 assay1 70.1.2 colony PCR 70.1.3 preculture 71 10,5,2014 71.1 Lab member 71.2 Contents 71.2.1 miniprep 71.2.2 preculture 72 10,6,2014 72.1 Lab member 72.2 Contents 72.2.1 assay1,3 72.2.2 Making M9 medium 72.2.3 result of OD600 72.2.4 assay1 72.2.5 preculture 73 10,7,2014 73.1 Lab member 73.2 Contents 73.2.1 assay 73.2.2 OD600 before subculture 73.2.3 PCR 73.2.4 PCR 74 10,8,2014 74.1 Lab member 74.2 Contents 74.2.1 making gel 74.2.2 subculture 74.2.3 PCR clean up and check 74.2.4 sequence order 74.2.5 TF 75 10,9,2014 75.1 Lab member 75.2 Contents 75.2.1 digestion,gel extraction 75.2.2 making gel 75.2.3 making plate 75.2.4 ligation 75.2.5 preculture 76 10,10,2014 76.1 Lab member 76.1.1 assay1,3 76.1.2 making M9 medium

Favorite Parts

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Name	Type	Description	Designer	Length
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461000" abp="49">BBa_K1461000</A>	RBS	crRBS	Takefumi Yoshikawa	52
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461001" abp="64">BBa_K1461001</A>	Regulatory	ecf20 promoter	Takefumi Yoshikawa	80
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461002" abp="79">BBa_K1461002</A>	Regulatory	ecf11 promoter	Takefumi Yoshikawa	80
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461003" abp="94">BBa_K1461003</A>	RNA	taRNA	Takefumi Yoshikawa	71
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461004" abp="109">BBa_K1461004</A>	Coding	ecf20	Takefumi Yoshikawa	582
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461005" abp="124">BBa_K1461005</A>	Coding	ecf11	Takefumi Yoshikawa	609
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461006" abp="139">BBa_K1461006</A>	Coding	anti ecf20	Takefumi Yoshikawa	432
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461007" abp="154">BBa_K1461007</A>	Coding	anti ecf11	Takefumi Yoshikawa	663

Other Pars

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</TBODY>

Name	Type	Description	Designer	Length
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461008" abp="194">BBa_K1461008</A>	Coding	GFP (AAV tag +)	Takefumi Yoshikawa	742
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461009" abp="209">BBa_K1461009</A>	Coding	GFP (AAV tag +)	Takefumi Yoshikawa	742
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461200" abp="329">BBa_K1461200</A>	Composite	taRNA (+d.term)	Takefumi Yoshikawa	208
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461201" abp="344">BBa_K1461201</A>	Composite	ecf20 (+RBS, +d.term)	Takefumi Yoshikawa	737
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461202" abp="359">BBa_K1461202</A>	Composite	ecf11 (+RBS, +d.term)	Takefumi Yoshikawa	764
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461203" abp="374">BBa_K1461203</A>	Composite	cr-ecf20 (+RBS, +d.term)	Takefumi Yoshikawa	777
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461204" abp="389">BBa_K1461204</A>	Composite	cr-ecf11 (+RBS, +d.term)	Takefumi Yoshikawa	804
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461205" abp="404">BBa_K1461205</A>	Composite	cr-GFP (+RBS, +d.term)	Takefumi Yoshikawa	915
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461206" abp="419">BBa_K1461206</A>	Composite	anti ecf20 (+RBS, +d.term)	Takefumi Yoshikawa	587
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461207" abp="434">BBa_K1461207</A>	Composite	anti ecf11 (+RBS, +d.term)	Takefumi Yoshikawa	818
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461300" abp="959">BBa_K1461300</A>	Composite	RBS-GFP-4miR-142-3p bindng sites-d.term	Shunsuke Sumi	1007
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461301" abp="974">BBa_K1461301</A>	Composite	RBS-GFP-4 miR-142-5p bindng sites-d.term	Shunsuke Sumi	999
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461302" abp="989">BBa_K1461302</A>	Composite	RBS-GFP-miR 142 3p non-binding site-d.term	Shunsuke Sumi	1001
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461303" abp="1004">BBa_K1461303</A>	Composite	RBS-GFP-miR 142 5p non-binding site-d.term	Shunsuke Sumi	1007
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461304" abp="1019">BBa_K1461304</A>	Composite	RBS-GFP-2miR 142 3p bindng sites-2miR 142 5p bindng sites-d.term	Shunsuke Sumi	1003
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461305" abp="1034">BBa_K1461305</A>	Composite	RBS-GFP-2miR 142 3p non-bindng sites-2miR 142 5p non-bindng sites-d.term	Shunsuke Sumi	1003
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461208" abp="449">BBa_K1461208</A>	Generator	taRNA (arabinose-induced) (+d.term)	Takefumi Yoshikawa	346
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461209" abp="464">BBa_K1461209</A>	Generator	arabinose-induced ecf20 generator	Takefumi Yoshikawa	875
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461210" abp="479">BBa_K1461210</A>	Generator	constitutive ecf20 generator	Takefumi Yoshikawa	780
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461211" abp="494">BBa_K1461211</A>	Generator	arabinose-induced ecf11 generator	Takefumi Yoshikawa	902
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461212" abp="509">BBa_K1461212</A>	Generator	constitutive ecf11 generator	Takefumi Yoshikawa	807
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461213" abp="524">BBa_K1461213</A>	Generator	arabinose-induced cr-ecf20 generator	Takefumi Yoshikawa	915
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461214" abp="539">BBa_K1461214</A>	Generator	constitutive cr-ecf20 generator	Takefumi Yoshikawa	820
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461215" abp="554">BBa_K1461215</A>	Generator	arabinose-induced cr-ecf11 generator	Takefumi Yoshikawa	942
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461216" abp="569">BBa_K1461216</A>	Generator	constitutive cr-ecf11 generator	Takefumi Yoshikawa	847
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461217" abp="584">BBa_K1461217</A>	Generator	arabinose-induced cr-GFP generator	Takefumi Yoshikawa	1053
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461218" abp="599">BBa_K1461218</A>	Generator	constitutive cr-GFP generator	Takefumi Yoshikawa	958
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461219" abp="614">BBa_K1461219</A>	Generator	ecf20-induced GFP generator	Takefumi Yoshikawa	963
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461220" abp="629">BBa_K1461220</A>	Generator	ecf11-induced GFP generator	Takefumi Yoshikawa	963
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461221" abp="644">BBa_K1461221</A>	Generator	ecf20 generator (positive feedback)	Takefumi Yoshikawa	825
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461222" abp="659">BBa_K1461222</A>	Generator	ecf11 generator (positive feedback)	Takefumi Yoshikawa	852
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461223" abp="674">BBa_K1461223</A>	Generator	anti ecf20 generator (pLac)	Takefumi Yoshikawa	795
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461224" abp="689">BBa_K1461224</A>	Generator	anti ecf11 generator (pLac)	Takefumi Yoshikawa	1026
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461225" abp="704">BBa_K1461225</A>	Measurement	ecf20 promoter measurement system	Takefumi Yoshikawa	1846
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461226" abp="719">BBa_K1461226</A>	Measurement	ecf11 promoter measurement system	Takefumi Yoshikawa	1873
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461231" abp="794">BBa_K1461231</A>	Measurement	arabinose-induced GFP and taRNA generator	Takefumi Yoshikawa	1407
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461232" abp="809">BBa_K1461232</A>	Measurement	single counter	Takefumi Yoshikawa	1312
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461233" abp="824">BBa_K1461233</A>	Measurement	ecf20 promoter mesurement system (positive feedback)	Takefumi Yoshikawa	2679
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461234" abp="839">BBa_K1461234</A>	Measurement	ecf11 promoter mesurement system (positive feedback)	Takefumi Yoshikawa	2733
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461235" abp="854">BBa_K1461235</A>	Measurement	resettable counter device using ecf20 (w/o crRBS, taRNA)	Takefumi Yoshikawa	3482
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461236" abp="869">BBa_K1461236</A>	Measurement	resettable counter device using ecf11 (w/o crRBS, taRNA)	Takefumi Yoshikawa	3767
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461237" abp="884">BBa_K1461237</A>	Measurement	sigma factors cross-talk assay 1	Takefumi Yoshikawa	1873
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461238" abp="899">BBa_K1461238</A>	Measurement	sigma factors cross-talk assay 2	Takefumi Yoshikawa	1846
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461227" abp="734">BBa_K1461227</A>	Project	resettable single counter system using ecf20	Takefumi Yoshikawa	2671
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461228" abp="749">BBa_K1461228</A>	Project	reset function using ecf20	Takefumi Yoshikawa	2576
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461229" abp="764">BBa_K1461229</A>	Project	resettable single counter system using ecf11	Takefumi Yoshikawa	2834
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461230" abp="779">BBa_K1461230</A>	Project	reset function using ecf11	Takefumi Yoshikawa	2929
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461239" abp="914">BBa_K1461239</A>	Project	σ-Re Counter (double, using ecf20)	Takefumi Yoshikawa	3821
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461240" abp="929">BBa_K1461240</A>	Project	σ-Re Counter (double, using ecf11)	Takefumi Yoshikawa	4106
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461241" abp="944">BBa_K1461241</A>	Project	σ-Re Counter (triple)	Takefumi Yoshikawa	6615
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461104" abp="284">BBa_K1461104</A>	Regulatory	EpCAM promoter	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461105" abp="299">BBa_K1461105</A>	Regulatory	EpCAM promoter(mutant)	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461100" abp="224">BBa_K1461100</A>	RNA	miR-142-3p target binding site	ドリキャス?	25
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461101" abp="239">BBa_K1461101</A>	RNA	miR-142-5p target bindng site	Shunsuke Sumi	23
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461102" abp="254">BBa_K1461102</A>	RNA	miRNA-142 binding site control1	Shunsuke Sumi	23
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461103" abp="269">BBa_K1461103</A>	RNA	miRNA-142 binding site control2	Shunsuke Sumi	25
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461306" abp="1049">BBa_K1461306</A>	Translational_Unit	pCMV-RBS-GFP-4 miR 142 3p site-d.term	Shunsuke Sumi	1595
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461307" abp="1064">BBa_K1461307</A>	Translational_Unit	pCMV-RBS-GFP-4 miR 142 5p site-d.term	Shunsuke Sumi	1587
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461308" abp="1079">BBa_K1461308</A>	Translational_Unit	pCMV-RBS-GFP-4 miR 142 3p non-binding sites-d.term	Shunsuke Sumi	1589
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461309" abp="1094">BBa_K1461309</A>	Translational_Unit	pCMV-RBS-GFP-4 miR 142 5p non-bindgin sites-d.term	Shunsuke Sumi	1595
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461310" abp="1109">BBa_K1461310</A>	Translational_Unit	pCMV-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term	Shunsuke Sumi	1591
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461311" abp="1124">BBa_K1461311</A>	Translational_Unit	pCMV-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term	Shunsuke Sumi	1591
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461312" abp="1139">BBa_K1461312</A>	Translational_Unit	pEpCAM-RBS-GFP-4 miR 142 3p site-d.term	Shunsuke Sumi	738
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461313" abp="1154">BBa_K1461313</A>	Translational_Unit	pEpCAM-RBS-GFP-4 miR 142 5p site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461314" abp="1169">BBa_K1461314</A>	Translational_Unit	pEpCAM-RBS-GFP-4 miR 142 3p non-bindng site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461315" abp="1184">BBa_K1461315</A>	Translational_Unit	pEpCAM-RBS-GFP-4 miR 142 5p non-bindng site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461316" abp="1199">BBa_K1461316</A>	Translational_Unit	pEpCAM-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461317" abp="1214">BBa_K1461317</A>	Translational_Unit	pEpCAM-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461318" abp="1229">BBa_K1461318</A>	Translational_Unit	pEpCAM(mutant)-RBS-GFP-4 miR 142 3p site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461319" abp="1244">BBa_K1461319</A>	Translational_Unit	pEpCAM(mutant)-RBS-GFP-4 miR 142 5p site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461320" abp="1259">BBa_K1461320</A>	Translational_Unit	pEpCAM(mutant)-RBS-GFP-4 miR 142 3p non-bindng site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461321" abp="1274">BBa_K1461321</A>	Translational_Unit	pEpCAM(mutant)-RBS-GFP-4 miR 142 5p non-bindng site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461322" abp="1289">BBa_K1461322</A>	Translational_Unit	pEpCAM(mutant)-RBS-GFP-2 miR 142 3p bindgin sites-2 miR 142 5p binding site-d.term	Shunsuke Sumi	?
<A href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1461323" abp="1304">BBa_K1461323</A>	Translational_Unit	pEpCAM(mutant)-RBS-GFP-2 miR 142 3p non-bindgin sites-2 miR 142 5p non-binding site-d.term	Shunsuke Sumi	?