Team:Cooper Union/Notebook/Biohack April

From 2014.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
<html>
<html>
<link rel="stylesheet" href="https://2014.igem.org/Team:Cooper_Union/CSS?action=raw&ctype=text/css" type="text/css" />
<link rel="stylesheet" href="https://2014.igem.org/Team:Cooper_Union/CSS?action=raw&ctype=text/css" type="text/css" />
-
<title>Cooper Union 2014 iGEM</title>
+
<link rel="stylesheet" href="https://2014.igem.org/Team:Cooper_Union/CSS/notebookStyles?action=raw&ctype=text/css" type="text/css" />
 +
<script type="text/javascript" src="https://2014.igem.org/Team:Cooper_Union/script/jquery?action=raw&ctype=application/javascript"></script>
 +
<script type="text/javascript" src="https://2014.igem.org/Team:Cooper_Union/script/notebookScript?action=raw&ctype=application/javascript" charset="utf-8"></script><title>Cooper Union 2014 iGEM</title>
<body>
<body>
Line 13: Line 15:
<ul class="menu">
<ul class="menu">
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/TdT_project">De Novo Synthesis </a></li>
-
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan</a> </li>
+
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Telomere_project">Programmable Lifespan Timer</a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Biohack_project">Biohacker Kit </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Hardware">OpenSource Hardware </a> </li>
 +
<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Parts">BioBrick Parts </a></li>
-
<!--<li class="menu"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Modeling">Modeling </a> </li>-->
 
</ul>
</ul>
</li>
</li>
-
<li class="menu-safety"> <a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li>
+
<li class="menu-safety"> Social
-
<li class="menu-outreach"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
+
<ul class="menu">
 +
<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Safety">Safety</a> </li>
 +
<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Ethics">Ethics</a> </li>
 +
<li class="menu"><a class="menu" href="https://2014.igem.org/Team:Cooper_Union/Outreach">Outreach </a></li>
 +
</ul>
 +
</li>
<li class="menu-notebook">  Notebook  
<li class="menu-notebook">  Notebook  
<ul class="menu">
<ul class="menu">
Line 41: Line 48:
</div>
</div>
-
<br>
+
 
-
<hr>
+
-
<br>
+
<div class="text">
<div class="text">
<!-----Add content below---->
<!-----Add content below---->
Line 113: Line 118:
-
<div class="cu-footer">
 
-
<div class="bottom-left">
 
-
<a href="https://2014.igem.org/Team:Cooper_Union" span="The Cooper Union: μToolbox"><img src="https://static.igem.org/mediawiki/2014/b/bd/CU_2014_logo.png" height="150"  /></a></div>
 
-
<div class="bottom-center">
 
-
<a href="https://2014.igem.org/Team:Cooper_Union/Project" span="Projects"><img src="https://static.igem.org/mediawiki/2014/2/27/CU_2014_LogoP.png" height="100" style="vertical-align: top;" title="Projects" /></a>
 
-
<a href="https://2014.igem.org/Team:Cooper_Union/Safety" span="Safety"><img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Safety" /></a>
 
-
<a href="https://2014.igem.org/Team:Cooper_Union/Outreach" span="Outreach"><img src="https://static.igem.org/mediawiki/2014/7/74/CU_2014_logoO.png" height="100" style="vertical-align: top;" title="Outreach" /></a>
 
-
<a href="https://2014.igem.org/Team:Cooper_Union/Notebook" span="Notebook"><img src="https://static.igem.org/mediawiki/2014/d/da/CU_2014_LogoN.png" height="100" style="vertical-align: top;" title="Notebook" /></a>
 
-
<a href="https://2014.igem.org/Team:Cooper_Union/biofanfic" span="Team"><img src="https://static.igem.org/mediawiki/2014/3/38/CU_2014_LogoT.png" height="100" style="vertical-align: top;" title="Team" /></a></div>
 
-
<div class="bottom-right">
 
-
<a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a>
 
-
</div>
 
 +
<div class="cu-footer">
 +
    <div class="bottom-left">
 +
        <a href="https://2014.igem.org/Team:Cooper_Union" span="The Cooper Union: μToolbox"><img src="https://static.igem.org/mediawiki/2014/b/bd/CU_2014_logo.png" height="150"  /></a></div>
 +
    <div class="bottom-center">
 +
        <a href="https://2014.igem.org/Team:Cooper_Union/Project" class="black" >
 +
            <img src="https://static.igem.org/mediawiki/2014/2/27/CU_2014_LogoP.png" height="100" style="vertical-align: top;" title="Projects" />
 +
            <span>Projects</span>
 +
        </a>
 +
        <a href="https://2014.igem.org/Team:Cooper_Union/Social" class="red" >
 +
            <img src="https://static.igem.org/mediawiki/2014/b/b2/CU_2014_logoS.png" height="100" style="vertical-align: top;" title="Social" />
 +
            <span>Social</span>
 +
        </a>
 +
        <a href="https://2014.igem.org/Team:Cooper_Union/Notebook" class="green" >
 +
            <img src="https://static.igem.org/mediawiki/2014/d/da/CU_2014_LogoN.png" height="100" style="vertical-align: top;" title="Notebook" />
 +
            <span>Notebook</span>
 +
        </a>
 +
        <a href="https://2014.igem.org/Team:Cooper_Union/biofanfic"class="blue">
 +
            <img src="https://static.igem.org/mediawiki/2014/3/38/CU_2014_LogoT.png" height="100" style="vertical-align: top;" title="Team" />
 +
            <span>Team</span>
 +
        </a>
 +
    </div>
 +
    <div class="bottom-right">
 +
        <a href="http://www.cooper.edu" target="_blank" span="The Cooper Union"><img src="https://static.igem.org/mediawiki/2014/9/90/CU_2014_Cooper_union_logo.png" height="150" /></a>
 +
    </div>
</div>
</div>

Latest revision as of 00:44, 18 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


3/13/14

  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII and EcoRI.

3/27/14

  • Ran digests from 3/13/14 on gel.
  • Excised gel fragments with plasmid in them.
  • Purified gel fragments.

4/10/14

Phosphatase treated the digested and double digested pBR322 from 3/13/14. 4/17/14
  • Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI.
  • Performed five ligations:
    pBR322 with UV
    pBR322 with HSP
    pBR322 with GFP
    UV with GFP
    HSP with GFP

4/24/14

  • Purified UV+GFP and HSP+GFP ligations.
  • Digested the two ligations with HindIII.
  • Ligated each one into pBR322.


Projects Social Notebook Team

Retrieved from "http://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_April"