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Team:NCTU Formosa/Notebook/test7
From 2014.igem.org
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[SacI-1]Cr</p><p> | [SacI-1]Cr</p><p> | ||
[AseI-1]Cr</p><p> | [AseI-1]Cr</p><p> | ||
| - | [ P<sub>cons</sub>+RBS+HindIII-1]Cr</p><p> | + | [P<sub>cons</sub>+RBS+HindIII-1]Cr</p><p> |
| - | [ P<sub>cons</sub>+RBS+KpnI-4]Cr</p><p> | + | [P<sub>cons</sub>+RBS+KpnI-4]Cr</p><p> |
| - | [ P<sub>cons</sub>+RBS+AseI-5]Cr</p><p> | + | [P<sub>cons</sub>+RBS+AseI-5]Cr</p><p> |
| - | [ P<sub>cons</sub>+RBS+XhoI-1]Cr</p><p> | + | [P<sub>cons</sub>+RBS+XhoI-1]Cr</p><p> |
| - | [ P<sub>cons</sub>+RBS+SacI-1]Cr</p><p> | + | [P<sub>cons</sub>+RBS+SacI-1]Cr</p><p> |
| - | [ P<sub>cons</sub>+RBS+KpnI-1+J61048]Cr</p><p> | + | [P<sub>cons</sub>+RBS+KpnI-1+J61048]Cr</p><p> |
[SalI-4]Cr</p><p> | [SalI-4]Cr</p><p> | ||
[P<sub>cons</sub>+RBS+SalI-3+J61048]Cr</p><p> | [P<sub>cons</sub>+RBS+SalI-3+J61048]Cr</p><p> | ||
| Line 226: | Line 226: | ||
<font color="yellow">Electrophoresis of</font></p><p> | <font color="yellow">Electrophoresis of</font></p><p> | ||
Gel 1</p><p> | Gel 1</p><p> | ||
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 P<sub>cons</sub>+RBS+NheI-20+RBS+BFP+TER</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+Hind-1+RBS+BFP+TER</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+KpnI-1+RBS+BFP+TER </p><p> | |
| - | + | Well 5 P<sub>cons</sub>+RBS+Ndel-1+RBS+BFP+TER</p><p>2 | |
| - | + | Well 6 P<sub>cons</sub>+RBS+NcoI-5+RBS+BFP+TER</p><p> | |
| - | + | Well 7 P<sub>cons</sub>+RBS+AseI ALL</p><p> | |
| - | + | Well 8 PBAN NcoI-12</p><p> | |
| - | + | Well 9 P<sub>cons</sub>+RBS+NheI-6</p><p> | |
| - | + | Well 10 P<sub>cons</sub>+RBS+Hind-1</p><p> | |
| - | + | Well 11 P<sub>cons</sub>+RBS+NcoI-20</p><p> | |
| - | + | Well 12 P<sub>cons</sub>+RBS+NcoI-2-2</p><p> | |
| - | + | Well 13 P<sub>cons</sub>+RBS+Ndel-2-1</p><p> | |
| - | + | Well 14 P<sub>cons</sub>+RBS+Nedl-2-2</p><p> | |
| - | + | Well 15 P<sub>cons</sub>+RBS+SacI-1</p><p> | |
| - | + | Well 16 P<sub>cons</sub>+RBS+SacI-11</p><p> | |
| - | + | Well 17 Marker</p><p> | |
Gel 2</p><p> | Gel 2</p><p> | ||
| - | + | Well 1 marker</p><p> | |
| - | + | Well 2 P<sub>cons</sub>+RBS+NheI-11</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+Hind3</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+KpnI-7</p><p> | |
| - | + | Well 5 P<sub>cons</sub>+RBS+SacI-16</p><p> | |
| - | + | Well 6 P<sub>cons</sub>+RBS+SalI-12</p><p> | |
| - | + | Well 7 P<sub>cons</sub>+RBS+Xhol</p><p> | |
<font color="yellow">Transformation of</font></p><p> | <font color="yellow">Transformation of</font></p><p> | ||
P<sub>cons</sub>+RBS+SacI-3 on psb3c5</p><p> | P<sub>cons</sub>+RBS+SacI-3 on psb3c5</p><p> | ||
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<font color="yellow">Electrophoresis of </font></p><p> | <font color="yellow">Electrophoresis of </font></p><p> | ||
| - | + | Well 1 Marker</p><p> | |
| - | + | Well 2 P<sub>cons</sub>+RBS+SacI-3 on psb3c5</p><p> | |
| - | + | Well 3 P<sub>cons</sub>+RBS+KpnI-1 on psb3c5</p><p> | |
| - | + | Well 4 P<sub>cons</sub>+RBS+NcoI-1 on psb3c5</p><p> | |
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<font color="yellow">PCR of and Electrophoresis of</font></p><p> | <font color="yellow">PCR of and Electrophoresis of</font></p><p> | ||
| - | + | Well 1 P<sub>cons</sub>+RBS+NcoI+psb3c5</p><p> | |
| - | + | Well 2 P<sub>cons</sub>+RBS+Ndel+psb3c5</p> | |
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
Latest revision as of 15:23, 17 October 2014
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10/3
Cultivation of
[Pcons+RBS+NdeI]Cr
[Pcons+RBS+NcoI]Cr
[KpnI-1]Cr
[NdeI-1]Cr
[XhoI-1]Cr
[SacI-1]Cr
[AseI-1]Cr
[Pcons+RBS+HindIII-1]Cr
[Pcons+RBS+KpnI-4]Cr
[Pcons+RBS+AseI-5]Cr
[Pcons+RBS+XhoI-1]Cr
[Pcons+RBS+SacI-1]Cr
[Pcons+RBS+KpnI-1+J61048]Cr
[SalI-4]Cr
[Pcons+RBS+SalI-3+J61048]Cr
[HindIII-4]Cr
[Pcons+RBS+AseI+J61048]Cr
[NheI]
[NcoI]
Electrophoresis of
[NdeI](EP)
[NcoI](EP)
[SacI](EP)
Transformation of
[Pcons+RBS+NdeI]Cr
[Pcons+RBS+SacI]Cr
DNA Sequencing of
[Pcons+RBS+NdeI+J61048-1]
[Pcons+RBS+HindIII+J61048-1]
[Pcons+RBS+SalI+J61048-4]
[Pcons+RBS+SacI+J61048-1]
[Pcons+RBS+SacI+J61048-1](fail)
10/4
Digestion of
[Pcons+RBS+XhoI+J61048-2, -4, -5]
[Pcons+RBS+SacI+J61048-10, -11]
[Pcons+RBS+NheI+J61048-20]
[Pcons+RBS+NheI]Cr
[Pcons+RBS+NcoI]Cr
Cultivation of
[Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]
[Pcons+RBS+SalI-1, -3, -6, -7, -11, -12]
Cultivation of
[Pcons+RBS+NcoI+J61048-1~8]
PCR of
[Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]
[Pcons+RBS+SacI-1, -3, -6, -7, -11, -12]
[Pcons+RBS+NcoI+J61048-1~8]
Ligation of
[Pcons+RBS+NheI]Kr
[Pcons+RBS+NcoI]Kr
Electrophoresis of
[XhoI-2, -4, -5](dig)
[SacI-10, -11](dig)
[NheI-20](dig)
[Pcons+RBS+NdeI](dig)
[Pcons+RBS+NcoI](dig)
[PCR NcoI-1~8]
Mini-prep of
[Pcons+RBS+NheI-6]
[Pcons+RBS+SalI-1, -7, -11]
Digestion of
[Pcons+RBS+NheI-6](EP)
[Pcons+RBS+SalI-1, -7, -11](EP)
[Pcons+RBS+NcoI]
[Pcons+RBS+Ndel]
PCR of and cultivation of
[Pcons+RBS+NcoI+J61048]
Mini-prep of
[Pcons+RBS+NcoI+J61048-7]Cr
Electrophoresis of
[Pcons+RBS+KphI+J61048-1]Cr
[Pcons+RBS+SalI+J61048-3]Cr
[Pcons+RBS+AseI+J61048 ]Cr
[Pcons+RBS+NdeI+J61048-1]
[Pcons+RBS+HindIII+J61048-1]
[Pcons+RBS+SacI+J61048 -1]
[Pcons+RBS+NcoI+J61048](dig)
[SacI-11]
DNA Sequencing of
[Pcons+RBS+NdeI]-2 Cr
[Pcons+RBS+NcoI]-2 Cr
[Pcons+RBS+NheI]-6 Cr
[Pcons+RBS+SalI-1]Cr
[Pcons+RBS+SalI-11]Cr
[Pcons+RBS+HindIII-1]Cr
[NcoI-12]
[Pcons+RBS+KpnI-1+J61048]Cr
[Pcons+RBS+AseI+J61048]Cr
[Pcons+RBS+SalI-3+J61048]Cr
[Pcons+RBS+HindIII-1+J61048]Cr
[Pcons+RBS+XhoI-2+J61048]Cr
[Pcons+RBS+XhoI-5+J61048]Cr
[Pcons+RBS+NcoI-5+J61048]Cr
[Pcons+RBS+SacI-11-1+J61048]Cr
[Pcons+RBS+NdeI-1+J61048]Cr
[Pcons+RBS+NheI-20+J61048]Cr
[Pcons+RBS+AseI-7+J61048]Cr
[Pcons+RBS+HindIII-7+J61048]Ar
[Pcons+RBS+SacI-16+J61048]Ar
[Pcons+RBS+SalI-17+J61048]Ar
[Pcons+RBS+NdeI-2+J61048]Cr
[Pcons+RBS+NcoI-2+J61048]Cr
[Pcons+RBS+XhoI+J61048]Ar
[Pcons+RBS+KpnI-7+J61048]Ar
[Pcons+RBS+NheI-11+J61048]Ar
10/7
PCR of
[Pcons+RBS+NheI+J61048]
[Pcons+RBS+SalI in PSB3C5]
[Pcons+RBS+SacI in PSB3C5]
Cultivation of
[Pcons+RBS+NheI+J61048-1, -3, -4, -6, -8, -10, -15, -16]
Transformation of
[Pcons+RBS+XhoI-2 +J61048]
[Pcons+RBS+XhoI-5 +J61048-1]
[Pcons+RBS+SacI-11+J61048]
PCR of
[Pcons+RBS+ NdeI in PSB3C5]
[Pcons+RBS+KpnI in PSB3C5]
[Pcons+RBS+AseI in PSB3C5]
[Pcons+RBS+XhoI in PSB3C5]
[Pcons+RBS+NcoI in PSB3C5]
[Pcons+RBS+NheI in PSB3C5]
nine kinds of PBAN
DNA Sequencing of
[Pcons+RBS+NcoI-5+J61048]
10/8
DNA Sequencing of
[PBAN NcoI]
[Pcons+RBS+SalI -1]
[Pcons+RBS+SalI-11]
[Pcons+RBS+NheI-6]
[Pcons+RBS+xhol-2+RBS+BFP+TER]
[Pcons+RBS+SacI-11+RBS+BFP+TER]
[Pcons+RBS+ Nhel-20+RBS+BFP+TER]
Ligation of
Pcons+RBS+NcoI on psb3c5
Pcons+RBS+NcoI on psb3c5
10/9
ALL Nhel-9(10/7)x2
ALL SacI-11(10/7)x1
ALL xhol-2(10/7)x1
Electrophoresis of
Gel 1
Well 1 Marker
Well 2 Pcons+RBS+NheI-20+RBS+BFP+TER
Well 3 Pcons+RBS+Hind-1+RBS+BFP+TER
Well 4 Pcons+RBS+KpnI-1+RBS+BFP+TER
Well 5 Pcons+RBS+Ndel-1+RBS+BFP+TER
2 Well 6 Pcons+RBS+NcoI-5+RBS+BFP+TER
Well 7 Pcons+RBS+AseI ALL
Well 8 PBAN NcoI-12
Well 9 Pcons+RBS+NheI-6
Well 10 Pcons+RBS+Hind-1
Well 11 Pcons+RBS+NcoI-20
Well 12 Pcons+RBS+NcoI-2-2
Well 13 Pcons+RBS+Ndel-2-1
Well 14 Pcons+RBS+Nedl-2-2
Well 15 Pcons+RBS+SacI-1
Well 16 Pcons+RBS+SacI-11
Well 17 Marker
Gel 2
Well 1 marker
Well 2 Pcons+RBS+NheI-11
Well 3 Pcons+RBS+Hind3
Well 4 Pcons+RBS+KpnI-7
Well 5 Pcons+RBS+SacI-16
Well 6 Pcons+RBS+SalI-12
Well 7 Pcons+RBS+Xhol
Transformation of
Pcons+RBS+SacI-3 on psb3c5
Pcons+RBS+KpnI-1 on psb3c5
Pcons+RBS+NcoI-1 on psb3c5
Mini-prep of and sequencing of
ALL NheI-2 x1
ALL SacI-11 x1
ALL Xhol-2 X1
10/10
Transformation of
Pcons+RBS+Ndel on psb3c5
Pcons+RBS+NcoI on psb3c5
Electrophoresis of
Well 1 Marker
Well 2 Pcons+RBS+SacI-3 on psb3c5
Well 3 Pcons+RBS+KpnI-1 on psb3c5
Well 4 Pcons+RBS+NcoI-1 on psb3c5
10/12
PCR of and Electrophoresis of
Well 1 Pcons+RBS+NcoI+psb3c5
Well 2 Pcons+RBS+Ndel+psb3c5
None
None
