Team:Groningen/Template/MODULE/Notebook/detection/week3

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11 August - 16 August
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August 11 - August 16
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K592025-B0015 was ligated to K1033225 and K1033222.
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Two ligation were performed, the first one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP44 (<a href="http://parts.igem.org/Part:BBa_K1033225">BBa_K1033225</a>), the second one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP29 (<a href="http://parts.igem.org/Part:BBa_K1033222">BBa_K1033222</a>)
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Both constructs were transformed to <i>E. coli</i> DH5α
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Blue coloured colonies were obtained.
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Some blue colonies were visible on the plates, an overnight culture was prepared from these colonies
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The plasmids were isolated from the blue coloured transformants
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The plasmids were isolated out of the overnight cultures
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The size was checked by restriction digestion
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The size of the constructs were digested, and checked on gel
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Latest revision as of 19:25, 17 October 2014

August 11 - August 16
 
Two ligation were performed, the first one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP44 (BBa_K1033225), the second one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP29 (BBa_K1033222)
 
Both constructs were transformed to E. coli DH5α
 
Some blue colonies were visible on the plates, an overnight culture was prepared from these colonies
 
The plasmids were isolated out of the overnight cultures
 
The size of the constructs were digested, and checked on gel
 
 
 
 

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