Team:Groningen/Template/MODULE/Notebook/detection/week1

From 2014.igem.org

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28 July - 3 August
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July 28 - August 3
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These Biobricks were transformed in the <i>E. coli</i>
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The following BioBricks were taken from the 2014 iGEM distribution kit, and transformed to <i>E. coli</i> DH5α
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<div class="item">B0015</div>
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<div class="item">The double terminator(<a href="http://parts.igem.org/Part:BBa_ B0015">BBa_ B0015</a>)</div>
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<div class="item">B0034</div>
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<div class="item">The RBS(<a href="http://parts.igem.org/Part:BBa_ B0034">BBa_ B0034</a>)</div>
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<div class="item">K592025</div>
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<div class="item">The gene for the purple-blue chromoprotein amilCP(<a href="http://parts.igem.org/Part:BBa_ K592025">BBa_ K592025</a>)</div>
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<div class="item">I746101</div>
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<div class="item">The genes for the AIP sensor infrastructure(<a href="http://parts.igem.org/Part:BBa_ I746101">BBa_ I746101</a>)</div>
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<div class="item">E0040</div>
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<div class="item">The gene coding for GFP(<a href="http://parts.igem.org/Part:BBa_ E0040">BBa_ E0040</a>)</div>
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<div class="item">S03156</div>
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<div class="item">The <i>lasR</i> gene(<a href="http://parts.igem.org/Part:BBa_ S03156">BBa_ S03156</a>)</div>
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<div class="item">R0079</div>
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<div class="item">The promoter LasR(<a href="http://parts.igem.org/Part:BBa_ R0079">BBa_ R0079</a>)</div>
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<div class="item">K081009</div>
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<div class="item">The <i>lasI</i> protein generator(<a href="http://parts.igem.org/Part:BBa_ K081009">BBa_ K081009</a>)</div>
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The samples were then digested and the size was checked
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All samples were digested, and checked on gel
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From all transformations a glycerol stock was made, and stored at - 80 °C
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Latest revision as of 19:39, 17 October 2014

July 28 - August 3
 
The following BioBricks were taken from the 2014 iGEM distribution kit, and transformed to E. coli DH5α
 
The double terminator(BBa_ B0015)
The RBS(BBa_ B0034)
The gene for the purple-blue chromoprotein amilCP(BBa_ K592025)
The genes for the AIP sensor infrastructure(BBa_ I746101)
The gene coding for GFP(BBa_ E0040)
The lasR gene(BBa_ S03156)
The promoter LasR(BBa_ R0079)
The lasI protein generator(BBa_ K081009)
 
 
All samples were digested, and checked on gel
 
From all transformations a glycerol stock was made, and stored at - 80 °C
 
 
 
 

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