Team:Groningen/Template/MODULE/projects/parts/biobricktable

iGEM Groningen 2014 assembled parts

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<th>EMPTY</th><th>Part</th><th>Type</th><th colspan="2">Description</th><th>Designer</th><th>Length</th>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365000">BBa_K1365000</a></td><td>Coding</td><td>nisA</td><td>Nisin precursor. Should be used in combination with the nisin gene cluster (e.g. Lactococcus lactis NZ9800).</td><td>Sandra Mous</td><td>174</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365003">BBa_K1365003</a></td><td>Coding</td><td>nisC</td><td>Protein needed in the cyclization of the nisin precursor. Part of the nisin gene cluster.</td><td>Sandra Mous</td><td>1245</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365006">BBa_K1365020</a></td><td>composite</td><td>nisRK</td><td>Two proteins involved in the recognition of nisin and positive feedback regulation to induce the pNisA promoter. This biobrick is part of the nisin gene cluster.</td><td>Sandra Mous</td><td>2023</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365020">BBa_K1365020</a></td><td>Coding</td><td>sfGFP</td><td>Superfolder GFP is a more stable protein than more communly used GFP. It gives an higher fluorescence as well1.</td><td>Sandra Mous</td><td>1625</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365100">BBa_K1365100</a></td><td>Device</td><td>EMPTY</td><td>Dual action system against S. aureus. System made for L. lactis and codes for nisin precursor and the biofilm degradeding enzyme DspB. The latter is fused with an USP45sp for secretion from the cell. This device has an P2 operon and this thus transcriped after binding from p-AgrA.</td><td>Rick Elbert</td><td>1625</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365111">BBa_K1365111</a></td><td>Coding</td><td>ssUsp45-dspB</td><td>DispersinB fused to an Usp45sp. The dspB enzyme is secreted from the cell via the usp system of L. lactis2.</td><td>Rick Elbert</td><td>1170</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365120">BBa_K1365120</a></td><td>Device</td><td>EMPTY</td><td>Dual action system against P. aeruginosa. System is made for L. lactis and includes the genes for autoinducer inactivation enzyme A (AiiA) and Dispersin B (DspB).</td><td>Rick Elbert</td><td>2350</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365130">BBa_K1365130</a></td><td>Device</td><td>EMPTY</td><td>EMPTY</td><td>Rick Elbert</td><td>EMPTY</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365169">BBa_K1365169</a></td><td>Coding</td><td>ssUsp45-aiiA</td><td>Autoinducer inactivation enzyme A (AiiA) gene fused to signal sequence for the usp45 secretion pathway.</td><td>Rick Elbert</td><td>834</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365301">BBa_K1365301</a></td><td>Plasmid</td><td>pIL253³</td><td>Plasmid for L. lactis including the biobrick compatible VF2 and RV primer binding sites, prefix and suffix. No illegal restriction sites.</td><td>Lisa Hielkema</td><td>6230</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365555">BBa_K1365555</a></td><td>Compesition</td><td>RBS-sfGFP-dt</td><td>Compesition of the superfolder GFP combined with RBS and double terminator (dt). This part was used to assemble with different promoters used for characterization experiments.</td><td>Aakanksha Saraf</td><td>874</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365556">BBa_K1365556

 

</a></td><td>Compesition</td><td>RBS-nisA-dt</td><td>Compesition of the precursor nisA combined with an RBS and double terminator (dt). This part was assembled with a range of promotor to be used for characterization experiments.</td><td>Aakanksha Saraf</td><td>328</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365557">BBa_K1365557</a></td><td>Reporter</td><td>EMPTY</td><td>ESuperfolder GFP under a constitituve promoter. Desingend for the use in L. lactis and E. coli.PTY</td><td>Aakanksha Saraf</td><td>915</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365997">BBa_K1365997</a></td><td>Device</td><td>EMPTY</td><td>AHL sensor. The LasR membrane protein is produced by this device. </td><td>Chandhuru Jagadeesan</td><td>1003</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365998">BBa_K1365998</a></td><td>Signaling</td><td>EMPTY</td><td>AHL generator. This part can be used to mimic the presence of AHL producing bacteria P. aeruginosa.</td><td>Chandhuru Jagadeesan</td><td>892</td>

<td>EMPTY</td><td><a href="http://parts.igem.org/Part:BBa_K1365999">BBa_K1365999</a></td><td>compesition</td><td>RBS-GFP-dt</td><td>Composition of ribosome binding site, green fluorescence protein and double terminator. Brick is both L. lactis as E. coli compatible.</td><td>Chandhuru Jagadeesan</td><td>873</td>

<td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td><td>EMPTY</td>