"
Team:Groningen/Template/MODULE/Notebook/detection/week1
From 2014.igem.org
(Difference between revisions)
Lisahielkema (Talk | contribs) (Created page with "<html> <!--Content module--> <div class="module contentmodule gridcell"> <div class="content"> <div class="wrapper"> <!-- TITLE SNIPPET START--> <div class="title"> 28 July - ...") |
|||
| (6 intermediate revisions not shown) | |||
| Line 9: | Line 9: | ||
<div class="title"> | <div class="title"> | ||
| - | + | July 28 - August 3 | |
</div> | </div> | ||
| Line 18: | Line 18: | ||
<div class="text"> | <div class="text"> | ||
| - | + | The following BioBricks were taken from the 2014 iGEM distribution kit, and transformed to <i>E. coli</i> DH5α | |
</div> | </div> | ||
| Line 28: | Line 28: | ||
<div class="listing"> | <div class="listing"> | ||
| - | <div class="item">B0015</div> | + | <div class="item">The double terminator(<a href="http://parts.igem.org/Part:BBa_ B0015">BBa_ B0015</a>)</div> |
| - | <div class="item">B0034</div> | + | <div class="item">The RBS(<a href="http://parts.igem.org/Part:BBa_ B0034">BBa_ B0034</a>)</div> |
| - | <div class="item">K592025</div> | + | <div class="item">The gene for the purple-blue chromoprotein amilCP(<a href="http://parts.igem.org/Part:BBa_ K592025">BBa_ K592025</a>)</div> |
| - | <div class="item">I746101</div> | + | <div class="item">The genes for the AIP sensor infrastructure(<a href="http://parts.igem.org/Part:BBa_ I746101">BBa_ I746101</a>)</div> |
| - | <div class="item">E0040</div> | + | <div class="item">The gene coding for GFP(<a href="http://parts.igem.org/Part:BBa_ E0040">BBa_ E0040</a>)</div> |
| - | <div class="item">S03156</div> | + | <div class="item">The <i>lasR</i> gene(<a href="http://parts.igem.org/Part:BBa_ S03156">BBa_ S03156</a>)</div> |
| - | <div class="item">R0079</div> | + | <div class="item">The promoter LasR(<a href="http://parts.igem.org/Part:BBa_ R0079">BBa_ R0079</a>)</div> |
| - | <div class="item">K081009</div> | + | <div class="item">The <i>lasI</i> protein generator(<a href="http://parts.igem.org/Part:BBa_ K081009">BBa_ K081009</a>)</div> |
<div class="hspacer"> </div> | <div class="hspacer"> </div> | ||
| Line 46: | Line 46: | ||
<div class="text"> | <div class="text"> | ||
| - | + | All samples were digested, and checked on gel | |
</div> | </div> | ||
| Line 52: | Line 52: | ||
<!-- PARAGRAPH SNIPPET END--> | <!-- PARAGRAPH SNIPPET END--> | ||
| + | <!-- PARAGRAPH SNIPPET START--> | ||
| + | <div class="text"> | ||
| + | From all transformations a glycerol stock was made, and stored at - 80 °C | ||
| + | |||
| + | </div> | ||
| + | <div class="hspacer"> </div> | ||
| + | <!-- PARAGRAPH SNIPPET END--> | ||
<div class="hspacer"> </div> | <div class="hspacer"> </div> | ||
</div> | </div> | ||
Latest revision as of 19:39, 17 October 2014
July 28 - August 3
The following BioBricks were taken from the 2014 iGEM distribution kit, and transformed to E. coli DH5α
The double terminator(BBa_ B0015)
The RBS(BBa_ B0034)
The gene for the purple-blue chromoprotein amilCP(BBa_ K592025)
The genes for the AIP sensor infrastructure(BBa_ I746101)
The gene coding for GFP(BBa_ E0040)
The lasR gene(BBa_ S03156)
The promoter LasR(BBa_ R0079)
The lasI protein generator(BBa_ K081009)
All samples were digested, and checked on gel
From all transformations a glycerol stock was made, and stored at - 80 °C
