Team:Groningen/Template/MODULE/Notebook/bandage/week13

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29 September - 3 October
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September 29 - October 3
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during the last few weeks there was one experiment I really wanted to do: Check whether l.lactis can get out of the gel on its own. We know that the pore-size is small enough to keep it encapsulated. but we still wanted to check it.
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During the last few week several time lapse experiments were performed, with all kind of concentration of polyacrylamide gel, and also with agar and agarose gells, in order to <i>L. lactis</i> grow in the gel  
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Small gels were poured and incubated overnight in GM17 at 30 °C, two types of gels were used, an intact one, and a fractured one, this might have some effect on releasing some <i>L. lactis</i> cells from the gel
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The way we did this experiment is by pouring a small part of gel and putting this overnight in a GM17 medium. we used 2 variables for this: one healthy gel without scratches or anything and one which was half fractured.
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Several percentages of polyacrylamide gels were made, from 2.5 % until 15 % (2.5 - 5 - 7.5 - 10 - 12.5 - 15), the intact gels showed no growth, therefore we can conclude that <i>L lactis</i> is not able to grow in any percentage of polyacrylamide gel
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This way the cells that were on the edge maybe came free.
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We've tried this with percentages varying from 2.5% until 15% (2.5 - 5 - 7.5 - 10 - 12.5 - 15).
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From 2.5 % until 15 % <i>L. lactis</i> was able to grow on/in the fractured polyacrylamide gel, no growth was show on the higher percentages
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all the healthy gels had no growth in the media, therefor we can conclude that our cells should be stuck.
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on the fractured gels it was quite different, I've expected that every percentage would have growth in the GM17 media.
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the 2.5% until 10% all had growth, 12.5% and 15% had no growth.
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Latest revision as of 03:41, 18 October 2014

September 29 - October 3
 
During the last few week several time lapse experiments were performed, with all kind of concentration of polyacrylamide gel, and also with agar and agarose gells, in order to L. lactis grow in the gel
 
Small gels were poured and incubated overnight in GM17 at 30 °C, two types of gels were used, an intact one, and a fractured one, this might have some effect on releasing some L. lactis cells from the gel
 
Several percentages of polyacrylamide gels were made, from 2.5 % until 15 % (2.5 - 5 - 7.5 - 10 - 12.5 - 15), the intact gels showed no growth, therefore we can conclude that L lactis is not able to grow in any percentage of polyacrylamide gel
 
From 2.5 % until 15 % L. lactis was able to grow on/in the fractured polyacrylamide gel, no growth was show on the higher percentages
 
 
 
 

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