Team:Groningen/Template/MODULE/Notebook/bandage/week10

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Fig. 5: One of the results from the diffusion tests. Here is clearly seen the range of nisin
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The result of one of the diffusion tests, the halos around the gels shows the range of inhibition
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8 September - 12 September
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September 8 - September 12
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Redone the diffusion experiments. This time after I put the acrylamid gel on the agar plate I've also put some liquid M17 agar around the edges to make sure everything will touch the agar. This was put overnight in the 30 °C stove. We were hoping for some nice halo's around the acrylamid gels.
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Performed the difussion experiment again, but using better conditions, the plate was incubated overnight at 30 °C
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As you can see on the picture this experiment worked as expected. Nisin's ability to create no growth area's are undoubtedly proven with these experiments. We've also tried this with putting the acrylamid circles for two hours in media or water. We expected that our cells would give a larger halo when grown with media. This result was different, there was no real visible difference between those two.
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Figure 4 shows the result of a diffusion experiment under improved conditions, the inhibition area around the gel patches indicate the diffusion of nisin through the polyacrylamide gell
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Latest revision as of 02:13, 18 October 2014

Figure 4
 
Figure 4: The result of one of the diffusion tests, the halos around the gels shows the range of inhibition
 
 
September 8 - September 12
 
Performed the difussion experiment again, but using better conditions, the plate was incubated overnight at 30 °C
 
Figure 4 shows the result of a diffusion experiment under improved conditions, the inhibition area around the gel patches indicate the diffusion of nisin through the polyacrylamide gell
 
 
 
 

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