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| - | {{Template:Team:DTU-Denmark/maintemplate}}
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| - | <html>
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| - |
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| - | <style>
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| - | #PoPScalculator table td {
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| - | border: 0px solid black;
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| - | }
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| - |
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| - | #PoPScalculator table {
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| - | background:transparent;
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| - | width:99.5%;
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| - |
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| - | background-color: transparent;
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| - | }
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| - |
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| - | #PoPScalculator table table {
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| - | padding-top:15px;
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| - | }
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| - |
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| - | #PoPScalculator table tr {
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| - | min-height: 20px;
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| - | }
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| - | </style>
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| - |
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| - | <body>
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| - | <div class="pagescontent">
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| - | <maincontent>
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| - |
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| - |
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| - |
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| - | <div id="PoPScalculator" style="width:800px;position:relative;">
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| - |
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| - | <table>
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| - | <tr height="169">
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| - | <td width="394";>
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| - | <table height="80px">
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| - | <tr>
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| - | <td width="200">
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| - | Fluorescence:
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| - | </td>
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| - | <td width="80" style="text-align:right; padding-top: 3px;">
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| - | <input name="fluorescence" type="number" step="any" min="0" placeholder="Enter number"style="width:80px" />
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| - | </td>
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| - | <td></td>
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| - | </tr>
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| - | <tr>
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| - | <td>
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| - | Standard curve:
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| - | </td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="slope" type="number" step="any" min="0" placeholder="slope" style="width:80px" />
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| - | </td>
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| - | <td></td>
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| - | </tr>
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| - |
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| - | <tr>
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| - | <td>
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| - | Standard curve:
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| - | </td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="intercept" type="number" step="any" min="0" placeholder="intercept" style="width:80px" />
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| - | </td>
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| - | <td></td>
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| - | </tr>
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| - |
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| - | <tr>
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| - | <td>
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| - | Spinach concentration:
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| - | </td>
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| - | <td style="text-align:right; padding-top: 3px;" width=80px">
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| - | <input name="concentration" type="number" step="any" min="0" placeholder="Enter number" value="0.45" style="width:80px" />
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| - | <td>µM</td>
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| - | </tr>
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| - | </table>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td width="394px" height="84">
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| - | <table height="80px">
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| - | <tr>
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| - | <td width="200" colspan="2">CFU count</td>
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| - | <td witdh="80" style="text-align:right; padding-top: 3px;">
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| - | <input name="CFU" type="number" step="any" min="0" placeholder="Count" style="width:80px" />
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td width="20" style="padding-top:7px"><input type="checkbox" name="CFUcheck"></td><td>Use OD measurement</td>
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| - | </tr>
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| - | </table>
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| - | </td>
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| - | <td width="395px">
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| - | <table>
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| - | <tr>
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| - | <td width="200">OD600</td>
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| - | <td width="80" style="text-align:right; padding-top: 3px;">
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| - | <input name="OD600" type="number" step="any" min="0" placeholder="Enter number" value="6.3" style="width:80px" />
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| - | </td>
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| - | </tr>
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| - | </table>
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| - | </td>
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| - | </tr>
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| - | <tr height="84">
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| - | <td>
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| - | <table>
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| - | <tr>
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| - | <td width="200" colspan="2">Degradation rate:</td>
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| - | <td width="80" style="text-align:right; padding-top: 3px;">
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| - | <input name="degen" type="number" step="any" min="0" placeholder="Enter number" value="0.083" style="width:80px" />
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| - | </td>
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| - | <td width="100" style="padding-top:4px">
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| - | <select name="degenunit">
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| - | <option value="3600">per hour</option>
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| - | <option value="60">per minute</option>
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| - | <option value="1">per second</option>
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| - | </select>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td width="20" style="padding-top:7px"><input type="checkbox" name="degcheck"></td><td colspan="2">Use degradation half time:</td>
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| - | </tr>
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| - | </table>
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| - | </td>
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| - | <td>
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| - | <table>
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| - | <tr>
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| - | <td width="200">Degradation half time:</td>
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| - | <td width="80" style="text-align:right; padding-top: 3px;">
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| - | <input name="deghalf" type="number" step="any" min="0" placeholder="Half time" style="width:80px" />
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| - | </td>
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| - | <td style="padding-top:4px">
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| - | <select name="deghalfunit">
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| - | <option value="3600">h</option>
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| - | <option value="60">m</option>
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| - | <option value="1">s</option>
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| - | </select>
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| - | </td>
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| - |
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| - | </tr>
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| - | </table>
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| - | </tr>
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| - | <tr height=84;>
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| - | <td>
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| - | <table>
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| - | <tr>
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| - | <td width="200" colspan="2">Growth rate:</td>
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| - | <td width="80" style="text-align:right; padding-top: 3px;">
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| - | <input name="growth" type="number" step="any" min="0" placeholder="Doubling time" value="0.83" style="width:80px" />
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| - | <td width="100" style="padding-top:4px">
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| - | <select name="growthunit">
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| - | <option value="3600">per hour</option>
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| - | <option value="60">per minute</option>
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| - | <option value="1">per second</option>
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| - | </select>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td width="20" style="padding-top:7px"><input type="checkbox" name="growthcheck"></td><td colspan="2">Use growth doubling time:<br></td>
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| - | </tr>
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| - | </table>
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| - | </td>
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| - | <td>
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| - | <table>
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| - | <tr>
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| - | <td>Growth doubling time:</td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="grodouble" type="number" step="any" min="0" placeholder="Doubling time" style="width:80px" />
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| - | <td width="20" style="padding-top:4px">
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| - | <select name="grodoubleunit">
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| - | <option value="3600">h</option>
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| - | <option value="60">m</option>
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| - | <option value="1">s</option>
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| - | </select>
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| - | </td>
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| - | </tr>
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| - | </table>
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| - | </tr>
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| - | <tr height=84>
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| - | <td>
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| - | <table>
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| - | <tr>
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| - | <td width="200">Fold-fraction:</td>
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| - | <td width="80" style="text-align:right; padding-top: 3px;">
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| - | <input name="foldfraction" type="number" step="any" min="0" placeholder="Enter number" value="50" style="width:80px" />
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| - | </td>
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| - | <td>%</td>
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| - | </tr>
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| - | <tr>
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| - | <td width="200">Copy number:</td>
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| - | <td width="80"style="text-align:right; padding-top: 3px;">
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| - | <input name="copynumber" type="number" step="any" min="0" placeholder="Enter number" value="300" style="width:80px" />
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| - | </td>
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| - | </tr>
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| - | </table>
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| - | </td>
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| - | </tr>
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| - | <tr height=231>
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| - | <td>
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| - | <table>
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| - |
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| - | </table>
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| - | </td>
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| - | </tr>
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| - | </table>
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| - |
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| - | </div>
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| - | <form onchange = "
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| - | if (fluorcheck.checked) { concentration.value = (parseFloat(fluorescence.value)-parseFloat(intercept.value)) / parseFloat(slope.value) };
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| - | if (CFUcheck.checked) { OD600.value = (parseFloat(CFU.value)*10000000000000) };
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| - | if (degcheck.checked) { degen.value = Math.log(2)/(parseFloat(deghalf.value)*parseFloat(deghalfunit.value)) };
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| - | if (growthcheck.checked) { growth.value = Math.log(2)/(parseFloat(grodouble.value)*parseFloat(grodoubleunit.value)) };
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| - | activity.value = (parseFloat(concentration.value)/parseFloat(OD600.value))*(602200/(parseFloat(foldfraction.value)/100))*((parseFloat(degen.value)/parseFloat(degenunit.value))+(parseFloat(growth.value)/parseFloat(growthunit.value)))/parseFloat(copynumber.value);
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| - |
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| - | activity.value = Math.round(parseFloat(activity.value)*1000)/1000;
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| - | ">
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| - |
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| - | <table border="0" cellspacing="5" cellpadding="0" style="width:100%;background: transparent;">
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| - |
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| - | <tr>
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| - |
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| - | <td width="165px" colspan="2">
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| - | Fluorescence:
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| - | </td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="fluorescence" type="number" step="any" min="0" placeholder="Enter number"style="width:80px" />
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| - | </td>
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| - | <td></td>
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| - | <td></td>
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| - | </tr>
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| - | <tr>
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| - | <td colspan="2">
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| - | Standard curve:
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| - | </td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="slope" type="number" step="any" min="0" placeholder="slope" style="width:80px" />
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| - | </td>
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| - | <td></td>
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| - | <td></td>
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| - | </tr>
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| - |
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| - | <tr>
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| - | <td colspan="2">
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| - | Standard curve:
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| - | </td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="intercept" type="number" step="any" min="0" placeholder="intercept" style="width:80px" />
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| - | </td>
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| - | <td></td>
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| - | <td></td>
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| - | </tr>
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| - |
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| - | <tr>
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| - | <td colspan="2">
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| - | Spinach concentration:
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| - | </td>
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| - | <td style="text-align:right; padding-top: 3px;" width=80px">
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| - | <input name="concentration" type="number" step="any" min="0" placeholder="Enter number" value="0.45" style="width:80px" />
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| - | <td>µM</td>
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| - | <td></td>
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| - | </tr>
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| - |
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| - | <tr>
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| - | <td colspan="2">OD600</td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="OD600" type="number" step="any" min="0" placeholder="Enter number" value="6.3" style="width:80px" />
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| - | </td>
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| - | <td></td>
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| - | <td width="15px"></td>
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| - | <td>CFU count</td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="CFU" type="number" step="any" min="0" placeholder="Count" style="width:80px" />
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| - | </td>
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| - | <td></td>
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| - | <td></td>
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| - | </tr>
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| - | <tr>
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| - | <td style="padding-top:7px"><input type="checkbox" name="CFUcheck"></td><td>Use CFU count</td>
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| - | </tr>
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| - | <tr>
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| - | <td colspan="2">Degradation rate:</td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="degen" type="number" step="any" min="0" placeholder="Enter number" value="0.083" style="width:80px" />
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| - | </td>
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| - | <td style="padding-top:4px">
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| - | <select name="degenunit">
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| - | <option value="3600">h-1</option>
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| - | <option value="60">m-1</option>
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| - | <option value="1">s-1</option>
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| - | </select>
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| - | </td>
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| - | <td></td>
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| - | <td>Degradation half time:</td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="deghalf" type="number" step="any" min="0" placeholder="Half time" style="width:80px" />
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| - | </td>
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| - | <td style="padding-top:4px">
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| - | <select name="deghalfunit">
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| - | <option value="3600">h</option>
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| - | <option value="60">m</option>
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| - | <option value="1">s</option>
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| - | </select>
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| - | </td>
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| - | <td></td>
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| - | </tr>
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| - | <tr>
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| - | <td style="padding-top:7px"><input type="checkbox" name="degcheck"></td><td colspan="2">Use degradation half time:</td>
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| - | </tr>
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| - | <tr>
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| - | <td colspan="2">Growth rate:</td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="growth" type="number" step="any" min="0" placeholder="Doubling time" value="0.83" style="width:80px" />
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| - | <td style="padding-top:4px">
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| - | <select name="growthunit">
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| - | <option value="3600">h-1</option>
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| - | <option value="60">m-1</option>
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| - | <option value="1">s-1</option>
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| - | </select>
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| - | </td>
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| - | <td></td>
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| - | <td>Growth doubling time:</td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="grodouble" type="number" step="any" min="0" placeholder="Doubling time" style="width:80px" />
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| - | <td style="padding-top:4px">
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| - | <select name="grodoubleunit">
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| - | <option value="3600">h</option>
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| - | <option value="60">m</option>
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| - | <option value="1">s</option>
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| - | </select>
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| - | </td>
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| - | <td></td>
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| - | </tr>
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| - | <tr>
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| - | <td style="padding-top:7px"><input type="checkbox" name="growthcheck"></td><td colspan="2">Use growth doubling time:<br></td>
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| - | </tr>
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| - | <tr>
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| - | <td colspan="2">Fold-fraction:</td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="foldfraction" type="number" step="any" min="0" placeholder="Enter number" value="50" style="width:80px" />
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| - | </td>
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| - | <td>%</td>
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| - | <td></td>
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| - | </tr>
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| - | <tr>
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| - | <td colspan="2">Copy number:</td>
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| - | <td style="text-align:right; padding-top: 3px;">
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| - | <input name="copynumber" type="number" step="any" min="0" placeholder="Enter number" value="300" style="width:80px" />
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| - | <td></td>
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| - | <td></td>
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| - | </td>
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| - | </tr>
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| - | <tr>
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| - | <td colspan="2">Promoter activity:</td>
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| - | <td style="text-align:right"><output name="activity" type="number" step="0.1"></output></td>
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| - | <td>PoPS</td>
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| - | <td></td>
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| - | </tr>
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| - | </table>
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| - |
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| - | <br>
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| - | <br>
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| - | <br>
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| - | <br>
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| - | <br>
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| - | <br>
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| - | <br>
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| - |
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| - | <br>
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| - |
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| - |
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| - |
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| - | </maincontent>
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| - | </div>
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| - | </body>
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| - |
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| - |
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| - |
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| - | </html>
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| - | {{:Template:Team:DTU-Denmark/footer}}
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"
