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Team:NEFU China/Biosafety
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<td><p> BBa_K1509003 </p></td> | <td><p> BBa_K1509003 </p></td> | ||
<td><p> A strong </p> | <td><p> A strong </p> | ||
| - | <p> | + | <p> constitutive promoter </p></td> |
<td><p> Synthesized by BGI Tech </p></td> | <td><p> Synthesized by BGI Tech </p></td> | ||
<td><p> regulate CDS7 </p> | <td><p> regulate CDS7 </p> | ||
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Latest revision as of 03:20, 18 October 2014
Biosafety
Overview
All of NEFU_China members completed safety training before the start of our project. The programs about safety training can be found here: http://dept.nefu.edu.cn/life/list/?227_1.html.(Chinese version). The Laboratory Biosafety Manual published by WHO has provided practical guidance for us. We work in a BSL1 lab equipped well to meet the safety criteria.
Q&A Section
- Q1. Please describe the chassis orgamisms you will be using in your project.
-
Species name
Risk Group
Risk Group Source
Disease risk to humans?
1
Synechococcus elongates PCC7942
1
No
2
Bacillus subtilis
1
It’s widely spread in the environment. And it is used in various areas and beneficial to human.
No
3
E.coli (DH5α)
1
www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia
No
4
E.coli (Rosetta pLysS)
1
www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia
No
- Q2. List and describe all new parts you will be using in your project.
-
Part name
Natural function of part
How did you acquire it?
How will you use it?
1
BBa_K1509000
Coding SmtB
Using PCR to isolate it from
Synechococcus elongates
PCC7942
Bind and regulate
smtO/P
2
BBa_K1509001
A regulator which is affected by
SmtB protein
Using PCR to isolate it from
Synechococcus elongates
PCC7942
Detect the heavy
metal ions with part1
3
BBa_K1509002
Mainly responsible for
flocculent activity
Using PCR to isolate it from Bacillus subtilis
Flocculate bacteria
to collect nanocrystals
4
BBa_K1509003
A strong
constitutive promoter
Synthesized by BGI Tech
regulate CDS7
gene expression
- Q3. Risks to the safety and health of team members or others working in the lab?
- We worked in a BSL1 lab and all of us had received the safety training. We have worked out an emergency package.
- Q4. What safety training have you received?
- In July, we completed Laboratory Biosafety Manual, Chemical/Fire/Electrical safety trainings before the start of our wet lab work. You can get more information here:http://dept.nefu.edu.cn/life/list/?227_1.html(Chinese version) If you have any questions, please contact us.
- Q5. Risks to the security through malicious misuse by individuals, groups, or countries?
- The biological materials we used have no menace and we have effective measures to prevent someone from misusing.
- Q6. If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?
- Our project includes special feature to reduce this risk. The flocculation module was designed to carry our project forward to commercial/industrial product.
- Q7. Does your country have national biosafety regulations or guidelines? If so, please provide a link to these regulations or guidelines if possible.
- Here you can see our national biosafety regulations and guidelines: http://www.stee.agri.gov.cn/biosafety(Chinese version) If you have any questions, please contact us.
