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Team:SUSTC-Shenzhen/Notebook/timeline.json
From 2014.igem.org
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{ | { | ||
"timeline": { | "timeline": { | ||
| - | "headline": "Lab | + | "headline": "Lab Notes", |
"type": "default", | "type": "default", | ||
"text": "<p>The steps to success.</p>", | "text": "<p>The steps to success.</p>", | ||
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"media": "https://static.igem.org/mediawiki/2014/2/22/Logo2014v2.png", | "media": "https://static.igem.org/mediawiki/2014/2/22/Logo2014v2.png", | ||
"credit": "iGEM SUSTC-Shenzhen", | "credit": "iGEM SUSTC-Shenzhen", | ||
| - | "caption": "Lab | + | "caption": "Lab Notes" |
}, | }, | ||
"date": [ | "date": [ | ||
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"tag": "CRISPR", | "tag": "CRISPR", | ||
"classname": "", | "classname": "", | ||
| - | "asset": { | + | "asset": {} |
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}, | }, | ||
{ | { | ||
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"endDate": "2014,8,10", | "endDate": "2014,8,10", | ||
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/PCR-for-mCherry'>PCR for mCherry</a>", | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/PCR-for-mCherry'>PCR for mCherry</a>", | ||
| - | "text": "<p>To get our target fragment | + | "text": "<p>To get our target fragment of mCherry by PCR</p>", |
"tag": "CRISPR", | "tag": "CRISPR", | ||
"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/thumb/0/00/SUSTC-Image-PCR-mCherry-1.jpg/800px-SUSTC-Image-PCR-mCherry-1.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
"credit": "Mengqi Xu, Sijia Liu", | "credit": "Mengqi Xu, Sijia Liu", | ||
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"tag": "CRISPR", | "tag": "CRISPR", | ||
"classname": "", | "classname": "", | ||
| - | "asset": { | + | "asset": {} |
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}, | }, | ||
{ | { | ||
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"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/thumb/0/0c/SUSTC-Shenzhen-Verification-pX330-140809-1.PNG/800px-SUSTC-Shenzhen-Verification-pX330-140809-1.PNG", |
"thumbnail": "", | "thumbnail": "", | ||
"credit": "Mengqi Xu, Sijia Liu", | "credit": "Mengqi Xu, Sijia Liu", | ||
| - | "caption": " | + | "caption": "DNA Gel" |
} | } | ||
}, | }, | ||
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"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/f/ | + | "media": "https://static.igem.org/mediawiki/2014//f/fd/SUSTC-Shenzhen-verification-for-pX330-mCherry-and-pX330-mCherry-GAL4.PNG", |
"thumbnail": "", | "thumbnail": "", | ||
"credit": "Mengqi Xu, Sijia Liu", | "credit": "Mengqi Xu, Sijia Liu", | ||
| Line 157: | Line 147: | ||
"tag": "CRISPR", | "tag": "CRISPR", | ||
"classname": "", | "classname": "", | ||
| - | "asset": { | + | "asset": {} |
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}, | }, | ||
{ | { | ||
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"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/f/ | + | "media": "https://static.igem.org/mediawiki/2014/f/f9/SUSTC-Shenzhen-varification-for-digested-plasmid.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
"credit": "Mengqi Xu, Sijia Liu", | "credit": "Mengqi Xu, Sijia Liu", | ||
| - | "caption": " | + | "caption": "Plates without Colonies" |
} | } | ||
}, | }, | ||
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"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/7/7c/SUSTC-Shenzhen-Verifications-for-succeed-Fill-in-plasmid.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
"credit": "Mengqi Xu, Sijia Liu", | "credit": "Mengqi Xu, Sijia Liu", | ||
| - | "caption": " | + | "caption": "DNA Gel" |
} | } | ||
}, | }, | ||
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"tag": "CRISPR", | "tag": "CRISPR", | ||
"classname": "", | "classname": "", | ||
| - | "asset": { | + | "asset": {} |
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}, | }, | ||
{ | { | ||
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"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/3/35/SUSTC-Shenzhen-Vatification-of-PCR-pointmutation-for-UAS.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
"credit": "Mengqi Xu, Sijia Liu", | "credit": "Mengqi Xu, Sijia Liu", | ||
| - | "caption": " | + | "caption": "DNA Gel" |
} | } | ||
}, | }, | ||
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"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/d/de/SUSTC-Shenzhen-Point-mutation-PCR-for-mCherry1.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
"credit": "Mengqi Xu, Sijia Liu", | "credit": "Mengqi Xu, Sijia Liu", | ||
| - | "caption": " | + | "caption": "DNA Gel under UV Light" |
} | } | ||
}, | }, | ||
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"tag": "CRISPR", | "tag": "CRISPR", | ||
"classname": "", | "classname": "", | ||
| - | "asset": { | + | "asset": {} |
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}, | }, | ||
{ | { | ||
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"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/8/85/SUSTC-Shenzhen-Verification-for-pX330-mCherry-pX330-mCherry-GAL4-by-enzyme-digestion.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
"credit": "Mengqi Xu, Sijia Liu", | "credit": "Mengqi Xu, Sijia Liu", | ||
| - | "caption": " | + | "caption": "DNA Gel" |
} | } | ||
}, | }, | ||
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"tag": "CRISPR", | "tag": "CRISPR", | ||
"classname": "", | "classname": "", | ||
| - | "asset": { | + | "asset": {} |
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}, | }, | ||
{ | { | ||
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"classname": "", | "classname": "", | ||
"asset": {} | "asset": {} | ||
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"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/2/2d/SUSTC-Shenzhen-5DayAfterTransefation-8.20G1.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
| - | "credit": " | + | "credit": "Lin Le", |
| - | "caption": " | + | "caption": "G1 pool cells on petri dish" |
} | } | ||
}, | }, | ||
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"asset": { | "asset": { | ||
"media": "https://static.igem.org/mediawiki/2014/c/cb/SUSTC-Shenzhen-PB5-Puro-TetOn-Cas9-PB3.png", | "media": "https://static.igem.org/mediawiki/2014/c/cb/SUSTC-Shenzhen-PB5-Puro-TetOn-Cas9-PB3.png", | ||
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"thumbnail": "", | "thumbnail": "", | ||
"credit": "ProfFan", | "credit": "ProfFan", | ||
| Line 358: | Line 300: | ||
"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/a/ac/SUSTC-Shenzhen-Blasticidin_testing_III3.png", |
"thumbnail": "", | "thumbnail": "", | ||
| - | "credit": " | + | "credit": "Lin Le", |
| - | "caption": " | + | "caption": "Strains of HeLa Cells" |
} | } | ||
}, | }, | ||
| Line 372: | Line 314: | ||
"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/2/2a/SUSTC-Shenzhen-Struggles-I-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-%26-7X-UAS-mCherry-pX330-1.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
| - | "credit": " | + | "credit": "Mengqi Xu, Sijia Liu", |
| - | "caption": " | + | "caption": "DNA Gel" |
} | } | ||
}, | }, | ||
| Line 386: | Line 328: | ||
"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/a/ae/SUSTC-Shenzhen-Purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL-1.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
| - | "credit": " | + | "credit": "Mengqi Xu, Sijia Liu", |
| - | "caption": " | + | "caption": "DNA Gel" |
} | } | ||
}, | }, | ||
| Line 400: | Line 342: | ||
"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/a/ae/SUSTC-Shenzhen-Purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL-1.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
"credit": "ProfFan", | "credit": "ProfFan", | ||
| Line 411: | Line 353: | ||
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-no-endotoxin-for-BIG-and-SMALL'>Plasmid purification no endotoxin for BIG and SMALL</a>", | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-no-endotoxin-for-BIG-and-SMALL'>Plasmid purification no endotoxin for BIG and SMALL</a>", | ||
"text": "<p>DH5α&BL21</p>", | "text": "<p>DH5α&BL21</p>", | ||
| + | "tag": "CRISPR", | ||
| + | "classname": "", | ||
| + | "asset": {} | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,8,30", | ||
| + | "endDate": "2014,8,30", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Construction_of_gRNA_replaceable_0X_2X_5X_7X_UAS-mCherry-pX330_plasmids'>Construction of gRNA replaceable 0X/2X/5X/7X-UAS-mCherry-pX330 plasmids</a>", | ||
| + | "text": "<p></p>", | ||
"tag": "CRISPR", | "tag": "CRISPR", | ||
"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/f/f6/SUSTC-Shenzhen-Construction-of-gRNA-replaceable-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
| - | "credit": " | + | "credit": "Mengqi Xu, Sijia Liu", |
| - | "caption": " | + | "caption": "DNA Gel" |
} | } | ||
}, | }, | ||
{ | { | ||
| - | "startDate": "2014,8, | + | "startDate": "2014,8,31", |
| - | "endDate": "2014,8, | + | "endDate": "2014,8,31", |
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids-and-Verification-with-AgeI-and-BbsI'>Plasmid purification for 0X,2X,5X,7X-UAS-mCherry-pX330 plasmids and Verification with AgeI and BbsI</a>", | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids-and-Verification-with-AgeI-and-BbsI'>Plasmid purification for 0X,2X,5X,7X-UAS-mCherry-pX330 plasmids and Verification with AgeI and BbsI</a>", | ||
"text": "<p>DH5α&BL21</p>", | "text": "<p>DH5α&BL21</p>", | ||
| Line 428: | Line 379: | ||
"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/f/f6/SUSTC-Shenzhen-Plasmid-purification-for-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids-and-Verification-with-AgeI-and-BbsI.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
| - | "credit": " | + | "credit": "Mengqi Xu, Sijia Liu", |
| - | "caption": " | + | "caption": "DNA Gel" |
} | } | ||
}, | }, | ||
| Line 442: | Line 393: | ||
"classname": "", | "classname": "", | ||
"asset": { | "asset": { | ||
| - | "media": "https://static.igem.org/mediawiki/2014/ | + | "media": "https://static.igem.org/mediawiki/2014/9/9b/SUSTC-Shenzhen-Construction-of-gRNA-for-HIV-or-HBV-carried-plasmids.jpg", |
"thumbnail": "", | "thumbnail": "", | ||
| - | "credit": " | + | "credit": "Mengqi Xu, Sijia Liu", |
| - | "caption": " | + | "caption": "DNA Gel" |
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,8,22", | ||
| + | "endDate": "2014,10,12", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Parts_building'>Building Necessary Parts</a>", | ||
| + | "text": "<p>We build everything.</p>", | ||
| + | "tag": "Fundamental", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/e/e2/2014_SUSTC-Shenzhen_gel_result_0930.2.png", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Rifei Chen", | ||
| + | "caption": "DNA Gel" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,9,8", | ||
| + | "endDate": "2014,10,16", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Biobricks_Characterization'>Characterization of Biobricks</a>", | ||
| + | "text": "<p>Digging deeper into the nature of biology.</p>", | ||
| + | "tag": "Fundamental", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/6/6c/SUSTC-Shenzhen_Characterization_9.28_Gel.jpg", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Peng Peng", | ||
| + | "caption": "DNA Gel" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,8,13", | ||
| + | "endDate": "2014,8,19", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/mCherry-BbsI-pointmutation'>mCherry BbsI point mutation</a>", | ||
| + | "text": "<p>one step for constructing a universal usable gRNA-insertion plasmid.</p>", | ||
| + | "tag": "Fundamental", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/d/de/SUSTC-Shenzhen-mCherry-BbsI-pointmutation-first-PCR-result.jpg", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Yicong Tao", | ||
| + | "caption": "DNA Gel" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,9,29", | ||
| + | "endDate": "2014,10,7", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Investigation-Cas9-protein-affect-cell-growth'>Investigation Cas9 protein affect cell growth</a>", | ||
| + | "text": "<p>Investigation of the effect of Cas9 protein on the cell growth(The safety of Cas9).</p>", | ||
| + | "tag": "HeLa Cell", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/a/a9/Figure_1._The_cell_grow_curve.png", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Lin Le", | ||
| + | "caption": "The Cell Growth Curve" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,9,13", | ||
| + | "endDate": "2014,9,21", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/G1_cells_transfected_with_px330'>Test of the function of Cas9</a>", | ||
| + | "text": "<p>Ensure the function of modified px330 targeting EGFP gene.</p>", | ||
| + | "tag": "HeLa Cell", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/4/4e/SUSTC-Shenzhen-G1_cells_without_pX330_transfection.png", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Lin Le", | ||
| + | "caption": "G1 Cells without pX330" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,9,13", | ||
| + | "endDate": "2014,9,24", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Test-of-the-clone-G3_6'>Test of the first clone of G3 (G3-6)</a>", | ||
| + | "text": "<p>Prepare for transfection with A-B toxin.</p>", | ||
| + | "tag": "HeLa Cell", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/4/4e/SUSTCSUSTC-Shenzhen-EGFP-mCherry-fluorescence-G3-6-microscope.png", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Lin Le", | ||
| + | "caption": "G3 under Fluorescence Microscope" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,9,20", | ||
| + | "endDate": "2014,10,7", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/The_quality_test_of_the_clones_of_G3%26G4'>The quality test of the clones of G3&G4</a>", | ||
| + | "text": "<p>Test the quality of clones we pick from G3 and G4.</p>", | ||
| + | "tag": "HeLa Cell", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/d/de/SUSTC-Shenzhen-monoclone-quality-G3-G4-negative-ctrl.png", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Lin Le", | ||
| + | "caption": "Normal HeLa Cells" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,9,14", | ||
| + | "endDate": "2014,9,25", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Examination-of-the-mCherry-expression-of-UAS_mCherry_gRNA'>Examination of the mCherry expression of the plasmids UAS-mCherry-gRNA</a>", | ||
| + | "text": "<p>Test the quality of UAS-mCherry-gRNA.</p>", | ||
| + | "tag": "HeLa Cell", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/c/cb/SUSTC-Shenzhen-UAS_test_47hours_transfect_cells.png", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Lin Le", | ||
| + | "caption": "Different Periods of G3 under FM" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,10,3", | ||
| + | "endDate": "2014,10,18", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/A-B-Toxin-transfect'>Transfection of cells with A-B toxin</a>", | ||
| + | "text": "<p>Introduce gRNA with TEG/GD5 protein.</p>", | ||
| + | "tag": "HeLa Cell", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/c/cb/SUSTC-Shenzhen-UAS_test_47hours_transfect_cells.png", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Lin Le", | ||
| + | "caption": "3 Days after Transfection" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,8,16", | ||
| + | "endDate": "2014,10,16", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/A-B_Toxin/Purify'>Mainstream A-B Toxin Preparation</a>", | ||
| + | "text": "<p>Purify the Protein and Meanwhile Improve the Protocol.</p>", | ||
| + | "tag": "A-B Toxin", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/4/46/SUSTC-Shenzhen-PWF47-TEG%28coding_sequence%29.jpg", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Longze Su", | ||
| + | "caption": "Coding Sequence" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,8,8", | ||
| + | "endDate": "2014,8,11", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLa_Cell/blasticidin_screening'>Blasticidin Screening</a>", | ||
| + | "text": "<p>To test the resistance of HeLa cell to blasticidin.</p>", | ||
| + | "tag": "HeLa Cell", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | "media": "https://static.igem.org/mediawiki/2014/2/26/Spread.jpg", | ||
| + | "thumbnail": "", | ||
| + | "credit": "Longze Su", | ||
| + | "caption": "3 Days after Transfection" | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,6,12", | ||
| + | "endDate": "2014,7,27", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Preparation_work'>Experiment Training & Plasmid Building</a>", | ||
| + | "text": "<p>Introduce gRNA with TEG/GD5 protein.</p>", | ||
| + | "tag": "Fundamental", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,9,22", | ||
| + | "endDate": "2014,10,4", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/insert-target-to-pBX-083'>PCR & Insert target-sequence to pBX-083</a>", | ||
| + | "text": "<p></p>", | ||
| + | "tag": "CRISPR", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
| + | } | ||
| + | }, | ||
| + | { | ||
| + | "startDate": "2014,8,27", | ||
| + | "endDate": "2014,8,27", | ||
| + | "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-blasticidin-for-cell-selection'>Determining the appropriate concentration of blasticidin for cell selection</a>", | ||
| + | "text": "<p></p>", | ||
| + | "tag": "HeLa Cell", | ||
| + | "classname": "", | ||
| + | "asset": { | ||
} | } | ||
} | } | ||
Latest revision as of 03:48, 18 October 2014
{
"timeline": {
"headline": "Lab Notes",
"type": "default",
"text": "The steps to success.
", "asset": {
"media": "
",
"credit": "iGEM SUSTC-Shenzhen",
"caption": "Lab Notes"
},
"date": [
{
"startDate": "2014,7,27",
"endDate": "2014,7,28",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Fundamental/TransEfficiency.htm'>Effiency Test</a>",
"text": "DH5α&BL21
", "tag": "Fundamental",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
},
{
"startDate": "2014,8,17",
"endDate": "2014,8,22",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-puromycin-for-cell-selection'>Determining the appropriate concentration of puromycin for cell selection</a>",
"text": "Prepare for selection
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Lidan Zeng",
"caption": "Our cells!"
}
},
{
"startDate": "2014,7,18",
"endDate": "2014,7,18",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-II-for-plasmid-pX330-EGFP'>Plasmid amplification II for pX330 & EGFP & verifications by electrophoresis</a>",
"text": "Prepare for selection
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Electrophoresis Snapshot"
}
},
{
"startDate": "2014,8,9",
"endDate": "2014,8,10",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Enzyme-digestion-agrose-gel-DNA-Extraction-for-PX330-vector'>Enzyme digestion & agrose gel DNA Extraction for PX330 vector</a>",
"text": "To prepare PX330 vector for the following ligation experiments
", "tag": "CRISPR",
"classname": "",
"asset": {}
},
{
"startDate": "2014,8,6",
"endDate": "2014,8,6",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/5*UAS-plasmids-Fill-in'>5*UAS plasmids Fill-in</a>",
"text": "To eliminate the EcoRI site on the 5*UAS plasmid
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,10",
"endDate": "2014,8,10",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/PCR-for-mCherry'>PCR for mCherry</a>",
"text": "To get our target fragment of mCherry by PCR
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,9",
"endDate": "2014,8,10",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-second-time'>UAS Fill-in (The second\ntime)</a>",
"text": "The same as the first Fill-in experiment
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,11",
"endDate": "2014,8,11",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Struggles-for-our-second-time-Fill-in'>Struggles for our second-time Fill-in</a>\n",
"text": "A real challenge, indeed.
", "tag": "CRISPR",
"classname": "",
"asset": {}
},
{
"startDate": "2014,8,11",
"endDate": "2014,8,11",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-plasmid-PX330-MCherry-GAL4-PX330-MCherry-amplified-2014-8-9-by-restriction-digestion'>Verification for plasmid PX330-MCherry-GAL4,PX330-MCherry (amplified on 2014.8.9) by restriction digestion</a>",
"text": "Failure, as it is.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,8,12",
"endDate": "2014,8,12",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-verification-of-plasmid-PX330-MCherry-GAL4'>Follow-up for verification of plasmid PX330-MCherry-GAL4 and The results of 'Struggles for fill-in(the second time)'</a>",
"text": "Life full of cycles, but each iteration with happiness.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Eluted DNA"
}
},
{
"startDate": "2014,8,12",
"endDate": "2014,8,12",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-Struggles-for-fill-in-the-second-time'>Follow-up for 'struggles for our second-time Fill-in'</a>",
"text": "Failure, again.
", "tag": "CRISPR",
"classname": "",
"asset": {}
},
{
"startDate": "2014,8,13",
"endDate": "2014,8,16",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-The-third-time'>UAS Fill-in (The-third-time)</a>",
"text": "Then try for another time.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "Plates without Colonies"
}
},
{
"startDate": "2014,8,14",
"endDate": "2014,8,14",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verifications-for-struggles-for-our-second-time-Fill-in'>Verifications for 'struggles for our second-time Fill-in'</a>",
"text": "Results verified.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,8,18",
"endDate": "2014,8,20",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Successful-fill-in-plasmid-(G56-1)-amplification'>Successful fill-in plasmid (G56-1) amplification</a>",
"text": "Days of success.
", "tag": "CRISPR",
"classname": "",
"asset": {}
},
{
"startDate": "2014,8,20",
"endDate": "2014,8,20",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-UAS'>Point-mutation PCR for UAS</a>",
"text": "One approach.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,8,21",
"endDate": "2014,8,21",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-mCherry-with-BbsI-site-mutation'>Point-mutation PCR for mCherry (with BbsI site mutation)</a>",
"text": "To eliminate the BbsI site on mCherry fragment.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel under UV Light"
}
},
{
"startDate": "2014,8,21",
"endDate": "2014,8,22",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Endotoxin-Free-plasmid-purification-for-pX330-mCherry-pX330-mCherry-GAL4'>Endotoxin Free plasmid purification for pX330+mCherry, pX330+mCherry+GAL4</a>",
"text": "To prepare for further cell culturing.
", "tag": "CRISPR",
"classname": "",
"asset": {}
},
{
"startDate": "2014,8,22",
"endDate": "2014,8,22",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-pX330-mCherry-pX330-mCherry-GAL4-enzyme-digestion'>Verification for pX330+mCherry, pX330+mCherry+GAL4 by enzyme digestion</a>",
"text": "To ensure that we have not made errors.
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,8,25",
"endDate": "2014,8,26",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Construction-5X-UAS-mCherry-pX330-7X-UAS-mCherry-pX330-I'>Construction of 5X-UAS-mCherry-pX330 & 7X-UAS-mCherry-pX330 I</a>",
"text": "",
"tag": "CRISPR",
"classname": "",
"asset": {}
},
{
"startDate": "2014,7,17",
"endDate": "2014,7,19",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-I-for-plasmid-pX330-EGFP'>Plasmid amplification I for plasmid pX330 & EGFP</a>",
"text": "Prepare the necessary materials
", "tag": "CRISPR",
"classname": "",
"asset": {}
},
{
"startDate": "2014,8,04",
"endDate": "2014,8,05",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Thawing-HeLa-Cell'>Thawing HeLa Cell</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {}
},
{
"startDate": "2014,8,14",
"endDate": "2014,9,20",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Construct-Cellline'>Constructing Cell Line</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Lin Le",
"caption": "G1 pool cells on petri dish"
}
},
{
"startDate": "2014,7,27",
"endDate": "2014,8,10",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/PB5-TetOn-Cas9-PB3-plasmid-construction'>PB5-TetOn-Cas9-PB3 Plasmid Construction</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
},
{
"startDate": "2014,9,27",
"endDate": "2014,10,06",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-higher-concentration-of-balsticidin-for-cell-selection'>Determining the Increased Concentration of Balsticidin for Cell Selection</a>",
"text": "DH5α&BL21
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Lin Le",
"caption": "Strains of HeLa Cells"
}
},
{
"startDate": "2014,8,26",
"endDate": "2014,8,26",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Struggles-I-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-and-7X-UAS-mCherry-pX330'>Struggles I for the first time construction of 5X-UAS-mCherry-pX330 and 7X-UAS-mCherry-pX330</a>",
"text": "DH5α&BL21
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,8,27",
"endDate": "2014,8,27",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-MU2-and-Struggles-II-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-and-7X-UAS-mCherry-pX330'>Plasmid purification for MU2 and Struggles II for the first time construction of 5X-UAS-mCherry-pX330 and 7X-UAS-mCherry-pX330</a>",
"text": "DH5α&BL21
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,8,28",
"endDate": "2014,8,28",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL'>Plasmid purification for Struggle I and Struggle II and Verification with AgeI EcoRI and BbsI for MU2 BIG and SMALL</a>",
"text": "DH5α&BL21
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "ProfFan",
"caption": "Figure 1"
}
},
{
"startDate": "2014,8,29",
"endDate": "2014,8,29",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-no-endotoxin-for-BIG-and-SMALL'>Plasmid purification no endotoxin for BIG and SMALL</a>",
"text": "DH5α&BL21
", "tag": "CRISPR",
"classname": "",
"asset": {}
},
{
"startDate": "2014,8,30",
"endDate": "2014,8,30",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Construction_of_gRNA_replaceable_0X_2X_5X_7X_UAS-mCherry-pX330_plasmids'>Construction of gRNA replaceable 0X/2X/5X/7X-UAS-mCherry-pX330 plasmids</a>",
"text": "",
"tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,8,31",
"endDate": "2014,8,31",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids-and-Verification-with-AgeI-and-BbsI'>Plasmid purification for 0X,2X,5X,7X-UAS-mCherry-pX330 plasmids and Verification with AgeI and BbsI</a>",
"text": "DH5α&BL21
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,10,6",
"endDate": "2014,10,10",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Construction_of_gRNA_for_HIV_or_HBV_carried_plasmids'>Construction of plasmids carrying the gRNA for HIV/HBV</a>",
"text": "DH5α&BL21
", "tag": "CRISPR",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Mengqi Xu, Sijia Liu",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,8,22",
"endDate": "2014,10,12",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Parts_building'>Building Necessary Parts</a>",
"text": "We build everything.
", "tag": "Fundamental",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Rifei Chen",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,9,8",
"endDate": "2014,10,16",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Biobricks_Characterization'>Characterization of Biobricks</a>",
"text": "Digging deeper into the nature of biology.
", "tag": "Fundamental",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Peng Peng",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,8,13",
"endDate": "2014,8,19",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/mCherry-BbsI-pointmutation'>mCherry BbsI point mutation</a>",
"text": "one step for constructing a universal usable gRNA-insertion plasmid.
", "tag": "Fundamental",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Yicong Tao",
"caption": "DNA Gel"
}
},
{
"startDate": "2014,9,29",
"endDate": "2014,10,7",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Investigation-Cas9-protein-affect-cell-growth'>Investigation Cas9 protein affect cell growth</a>",
"text": "Investigation of the effect of Cas9 protein on the cell growth(The safety of Cas9).
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Lin Le",
"caption": "The Cell Growth Curve"
}
},
{
"startDate": "2014,9,13",
"endDate": "2014,9,21",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/G1_cells_transfected_with_px330'>Test of the function of Cas9</a>",
"text": "Ensure the function of modified px330 targeting EGFP gene.
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Lin Le",
"caption": "G1 Cells without pX330"
}
},
{
"startDate": "2014,9,13",
"endDate": "2014,9,24",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Test-of-the-clone-G3_6'>Test of the first clone of G3 (G3-6)</a>",
"text": "Prepare for transfection with A-B toxin.
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Lin Le",
"caption": "G3 under Fluorescence Microscope"
}
},
{
"startDate": "2014,9,20",
"endDate": "2014,10,7",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/The_quality_test_of_the_clones_of_G3%26G4'>The quality test of the clones of G3&G4</a>",
"text": "Test the quality of clones we pick from G3 and G4.
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Lin Le",
"caption": "Normal HeLa Cells"
}
},
{
"startDate": "2014,9,14",
"endDate": "2014,9,25",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Examination-of-the-mCherry-expression-of-UAS_mCherry_gRNA'>Examination of the mCherry expression of the plasmids UAS-mCherry-gRNA</a>",
"text": "Test the quality of UAS-mCherry-gRNA.
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Lin Le",
"caption": "Different Periods of G3 under FM"
}
},
{
"startDate": "2014,10,3",
"endDate": "2014,10,18",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/A-B-Toxin-transfect'>Transfection of cells with A-B toxin</a>",
"text": "Introduce gRNA with TEG/GD5 protein.
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "",
"thumbnail": "",
"credit": "Lin Le",
"caption": "3 Days after Transfection"
}
},
{
"startDate": "2014,8,16",
"endDate": "2014,10,16",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/A-B_Toxin/Purify'>Mainstream A-B Toxin Preparation</a>",
"text": "Purify the Protein and Meanwhile Improve the Protocol.
", "tag": "A-B Toxin",
"classname": "",
"asset": {
"media": "
",
"thumbnail": "",
"credit": "Longze Su",
"caption": "Coding Sequence"
}
},
{
"startDate": "2014,8,8",
"endDate": "2014,8,11",
"headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLa_Cell/blasticidin_screening'>Blasticidin Screening</a>",
"text": "To test the resistance of HeLa cell to blasticidin.
", "tag": "HeLa Cell",
"classname": "",
"asset": {
"media": "
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