Team:ITESM-CEM/Parts
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<html> | <html> | ||
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+ | <link href='http://fonts.googleapis.com/css?family=Exo:300,400,600' rel='stylesheet' type='text/css'> | ||
+ | <link href='http://fonts.googleapis.com/css?family=Open+Sans:400italic,700italic,400,700' rel='stylesheet' type='text/css'> | ||
+ | <title>TEC-CEM | Parts</title> | ||
+ | <meta http-equiv="Content-Type" content="text/html; charset=utf-8"> | ||
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<style type="text/css"> | <style type="text/css"> | ||
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+ | <body leftmargin="0" topmargin="0" marginwidth="0" marginheight="0"> | ||
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- | < | + | <table border="0" align="center" cellpadding="0" cellspacing="0" id="Table_01" style="background:transparent;"> |
- | + | <tr> | |
- | + | <td colspan="3" valign="bottom"><h3>ITESM-CEM | Enzy7-K me</h3></td> | |
- | < | + | <td colspan="2" rowspan="2" align="right"> |
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- | < | + | <div class="header"> |
- | < | + | <iframe src="https://2014.igem.org/Team:ITESM-CEM/HEADER" width="235" height="77" frameborder="0" scrolling="no" seamless></iframe> |
- | + | </div> | |
- | < | + | </td> |
- | <td | + | <td><img src="images/spacer.gif" width="1" height="43" alt=""></td> |
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="3" rowspan="2"><h1>Parts | ||
+ | <h1n> 3256</h1n></h1></td> | ||
+ | <td><img src="images/spacer.gif" width="1" height="34" alt=""></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td rowspan="4"> </td> | ||
+ | <td rowspan="4" align="right" valign="top"> | ||
- | < | + | <div class="headerSocial"> |
- | <tr | + | <iframe src="https://2014.igem.org/Team:ITESM-CEM/SOCIAL" width="165" height="34" frameborder="0" scrolling="no" seamless></iframe> |
- | <tr | + | </div> |
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+ | <td><img src="images/spacer.gif" width="1" height="6" alt=""></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="3" rowspan="3" align="left" valign="top"><ul> | ||
+ | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Parts" style="color: #FFF;">Our Parts</a></sub> | ||
+ | <sub><a href="https://2014.igem.org/Team:ITESM-CEM/List">List of our parts</a></sub> | ||
+ | </ul></td> | ||
+ | <td><img src="images/spacer.gif" width="1" height="1" alt=""></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><img src="images/spacer.gif" width="1" height="3" alt=""></td> | ||
+ | </tr> | ||
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+ | <td><img src="images/spacer.gif" width="1" height="28" alt=""></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="5" valign="top"> | ||
+ | <table width="100%" border="0" id="ContenidoSecciones"> | ||
+ | <div style="background-color: #f3f3e2; style="width:95%"> | ||
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- | < | + | <!--INICIO CONTENIDO--> |
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- | < | + | <h2>Our Parts</h2> |
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- | < | + | <iframe align="right" width="660" height="515" src="//www.youtube.com/embed/1mFwPDbb7UY" frameborder="0" allowfullscreen></iframe> |
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- | < | + | <p style="text-align: justify; text-justify: inter-word;"> The main goal of our project was to establish the construct which will help us to metabolize 7-ketocholesterol, consisting in the use of three specific enzymes, but for further applications we submitted them in single modules. This will serve as the basis of a future library for standardized work related to atherosclerosis. |
- | < | + | </p><br> |
- | < | + | <h2>Cholesterol Oxidase</h2> |
- | + | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was first detected in <u>Chromobacterium sp.</u> We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1871 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin. | |
+ | </p> <br> | ||
- | < | + | <h2>Oxoacyl Reductase</h2> |
- | <a | + | <p style="text-align: justify; text-justify: inter-word;"> This enzyme was detected in <u>Rhodococcus jostii </u>. We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 1007 nucleotides and its codons were optimized in order to use it on <u>E. coli</u>, it already included a stop codon for transcription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin. |
+ | </p> <br> | ||
- | < | + | <h2>7-dehydratase</h2> |
- | <a | + | <p style="text-align: justify; text-justify: inter-word;"> This enzyme (7-alpha dehydratase) was detected in <u>Rhodococcus jostii</u> . We introduced it in a plasmid backbone with chloramphenicol resistance: pSB1C3. Its length is of 602 nucleotides and its codons were optimized in order to use it on <u>E.coli</u>, it already included a stop codon for trancription, it was also modified by the addition of a glycosilation site (NIT) and the peptide signal of human S-cathepsin. |
+ | </p> <br> | ||
+ | <h2>Neomycin Resistance</h2> | ||
+ | <p style="text-align: justify; text-justify: inter-word;"> This selective marker was obtained from an mammalian expression vector. NeoR's length is 855 nucleotides and it was isolated from pcDNA3.1(-)/myc-His A. | ||
+ | </p><br> | ||
- | < | + | <h2>BGHPA</h2> |
- | </ | + | <p style="text-align: justify; text-justify: inter-word;"> Bovine Growth Hormone Polyadenilation Signal for nuclease resistance. Translation terminator for eukaryotic cells. |
- | < | + | </p><br> |
- | < | + | <h2>PCMV</h2> |
- | </ | + | <p style="text-align: justify; text-justify: inter-word;"> Constitutive promoter from Cytomegalovirus, this promoter works on eukaryotic cells, driving protein expression. |
+ | </p><br><br><br> | ||
+ | <br> | ||
- | < | + | <!--FIN CONTENIDO--> |
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+ | </div> | ||
+ | </table> | ||
+ | <p> </p></td> | ||
+ | <td><img src="images/spacer.gif" width="1" height="509" alt=""></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><img src="images/spacer.gif" width="240" height="1" alt=""></td> | ||
+ | <td><img src="images/spacer.gif" width="544" height="1" alt=""></td> | ||
+ | <td><img src="images/spacer.gif" width="133" height="1" alt=""></td> | ||
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+ | <td><img src="images/spacer.gif" width="165" height="1" alt=""></td> | ||
+ | <td></td> | ||
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+ | <table width="100%" border="0" align="center" cellpadding="0" cellspacing="0" id="Table_" dwcopytype="CopyTableRow"> | ||
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+ | <td colspan="5" align="left" valign="bottom"> | ||
+ | <div class="footer"> | ||
+ | <iframe src="https://2014.igem.org/Team:ITESM-CEM/FOOTER" width="500" height="82" frameborder="0" scrolling="no" seamless></iframe></div> | ||
+ | </td> | ||
+ | <td width="37%" align="right" valign="bottom"> | ||
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+ | <div class="PLASMENU"> | ||
+ | <iframe src="https://2014.igem.org/Team:ITESM-CEM/POPMENU" width="614" height="614" frameborder="0" scrolling="no" seamless></iframe> | ||
+ | </div> | ||
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Latest revision as of 02:12, 18 October 2014
ITESM-CEM | Enzy7-K me |
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