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Team:TU Darmstadt/Materials/Primers
From 2014.igem.org
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Latest revision as of 00:27, 18 October 2014
Pathway
| RBS_CHI_pre |
| GTAGAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTCCTCCAGTGTCCGTTAC |
| RBS_CHS_pre |
| GTAGAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCGTCCTCCGTTGACATGAAGGCG |
| CHI_psb1c3_Pre |
| TAATAAGAATTCGCGGCCGCTTCTAGATGGCTCCTCCAGTGTCCGTTAC |
| CHI_psb1c3_Suf2 |
| TTATTACTGCAGCGGCCGCTACTAGTACTAGACTCCAATCACTGGAATAGTAGATTTCTCGG |
| CHS_psb1c3_Pre2 |
| TAATAAGAATTCGCGGCCGCTTCTAGATGGCGTCCTCCGTTGACATGAAGGCGATCAGAG |
| CHS_pscb1c3_Suf2 |
| TTATTACTGCAGCGGCCGCTACTAGTATTAGACGGGAACCGTCACGGTGGTTGGGAC |
| QCIII_CHI_F |
| GAAGTTTCTGGATGTTTTCCGGAGTGAAACTTTTCCACCTGG |
| QCIII_CHI_R |
| CCAGGTGGAAAAGTTTCACTCCGGAAAACATCCAGAAACTTC |
| QCI_CHI_F |
| CGGCGATAGGTGTGTATCTGGAAGAGAGTGCTATTCCTTTTCTGGCCG |
| QCI_CHI_R |
| CGGCCAGAAAAGGAATAGCACTCTCTTCCAGATACACACCTATCGCCG |
| DFR_mut_EcoRI_577_F |
| TACGCAGCAGAAAACAATCTCGAATTTATCAGTATCATTCCACCGC |
| DFR_mut_EcoRI_577_R |
| ATGCGTCGTCTTTTGTTAGAGCTTAAATAGTCATAGTAAGGTGGCG |
| DFR_mut2_EcoRI_622_F |
| CGCCACCTTACTATGACTATTTAAGCTCTAACAAAAGACGACGCAT |
| DFR_mut2_EcoRI_622_R |
| GCGGTGGAATGATACTGATAAATTCGAGATTGTTTTCTGCTGCGTA |
| DFR_Prefix |
| GCGCGAATTCGCGGCCGCTTCTAGATGGTTTCTAGTACAATTAACGAGAC |
| DFR_Suffix |
| GCGCTACTAGTAGCGGCCGCTGCAGTTAAGCAGATCCATTTTCATGATGT |
| DFR_RBS_Prefix |
| GTAGGAATTCGCGGCCGCTTCTAGAAAGAGGAGAAATACTAGATGGTTTCTAGTACAATTAACGAGACAC |
| F3H_Prefix |
| GATGGAATTCGCGGCCGCTTCTAGATGGCTCCTTCAACTCTCAC |
| F3H_RBS_Prefix |
| GATGGAATTCGCGGCCGCTTCTAGAAAGAGGAGAAATACTAGATGGCTCCTTCAACTCTCAC |
| F3H_Suffix |
| GCGCCTGCAGCGGCCGCTACTAGTATCAAGCGAAAATTTCGTCTGCG |
| F3H_mut_EcoRI_fwd_820_F |
| GCCGACCACCAAGCAGTTGTGAACTCCAATTCTAGCAGAATGTCAAT |
| F3H_mut_EcoRI_fwd_820_R |
| ATTGACATTCTGCTAGAATTGGAGTTCACAACTGCTTGGTGGTCGGC |
| F3H_mut_PstI_fwd_1047_F |
| GAAGTGGAGAAGGCCAAGCTGCAAATGACACCCAAGAGC |
| F3H_mut_PstI_fwd_1047_R |
| GCTCTTGGGTGTCATTTGCAGCTTGGCCTTCTCCACTTC |
| eANS_reverse |
| ATAATTCTGCAGCGGCCGCTACTAGTAATGATTTTTTCTTTTGGCGGTTCACAAAACAC |
Scaffold
| Scaff pET21a+ up |
| ATATCATATGGGATCTACCAAAGCAGATATTGGCAC |
| Scaff pET21a+ lo |
| ATATCTCGAGAGATCCGCTGCCGCTCCCACTA |
| Scaff-His up 2 right |
| ATGCTAGGAATTCGCGGCCGCTTCTAGATGGGATCTACCAAAGCAGATATTGGCACC |
| Scaff-His lo |
| TATACTGCAGCGGCCGCTACTAGTATTAGTGGTGGTGGTGGTGGTGCTCG |
| SHC up |
| ATATGAATTCGCGGCCGCTTCTAGATGGGATCTACCAAAGCAGATATTGGC |
| SHC lo |
| ATATCTGCAGCGGCCGCTACTAGTATCAGCAGTGGTGGTGGTGGTG |
| SH3-F |
| ATGCTAGGAATTCGCGGCCGCTTCTAGAAGATCTGCCGAATATGTTCGTGCACTGTTTGA |
| SH3-R |
| TCATCACTGCAGCGGCCGCTACTAGTAGGATCCTGAGCCACTACCACTACC |
| PDZ-F |
| ATGCTAGGAATTCGCGGCCGCTTCTAGAAGATCTCTGCAACGTCGTCGTGTTACCGTTCG |
| PDZ-R |
| TCATCACTGCAGCGGCCGCTACTAGTAGGATCCGCTGCCGCTCCCACTACCG |
| GBD-Met-F |
| ATGCTAGGAATTCGCGGCCGCTTCTAGATGAGATCTACCAAAGCAGATATTGGCACC |
| GBD-R |
| TCATCACTGCAGCGGCCGCTACTAGTAGGATCCGCTACCACTGCCGCTACCTGAACC |
| Prefix |
| GTTTCTTCGAATTCGCGGCCGCTTCTAG |
| Suffix |
| GTTTCTTCCTGCAGCGGCCGCTACTAGTA |
Safety
| hokD-fw |
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAGCAGCAAAAGGCGATGTTAATCG |
| hokD-rev |
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTCCTCAGGTTCGTAAGCTGTGAAG |
