Team:Groningen/Template/MODULE/Notebook/characterisation/week5

From 2014.igem.org

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29 September - 5 September
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September 29 - October 5
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Testing the promotor collection ( CP1, CP11, CP29, CP30, CP41, CP44) with NisA-terminator-RBS and Superfolded GFP-terminator-RBS
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Testing the promoter collection containing CP1 (<a href="http://parts.igem.org/Part:BBa_K1033219">BBa_K1033219</a>), CP11 (<a href="http://parts.igem.org/Part:BBa_K1033220">BBa_K1033220</a>), CP29 (<a href="http://parts.igem.org/Part:BBa_K1033221">BBa_K1033221</a>), CP30 (<a href="http://parts.igem.org/Part:BBa_K1033223">BBa_K1033223</a>), CP41 (<a href="http://parts.igem.org/Part:BBa_K1033224">BBa_K1033224</a>) and CP44 (<a href="http://parts.igem.org/Part:BBa_K1033225">BBa_K1033225</a>), and testing the newly formed RBS-NisA-double terminator (<a href="http://parts.igem.org/Part:BBa_K1365556">BBa_K1365556</a>) and RBS-Superfolded GFP-double terminator (<a href="http://parts.igem.org/Part:BBa_K1365555">BBa_K1365555</a>) BioBricks
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<div class="item">Nis A-terminator-RBS was ligated only with CP29 and CP44 from the promoter collection</div>
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<div class="item">The RBS-NisA-double terminator BioBrick was ligated with CP29 and CP44 from the promoter collection</div>
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<div class="item">Superfolded GFP- terminator-RBS was ligated with all the promotors from the collection.</div>
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<div class="item">The RBS-Superfolded GFP-double terminator BioBricks was ligated with all the promoters of the promoter collection</div>
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Latest revision as of 17:58, 17 October 2014

September 29 - October 5
 
Testing the promoter collection containing CP1 (BBa_K1033219), CP11 (BBa_K1033220), CP29 (BBa_K1033221), CP30 (BBa_K1033223), CP41 (BBa_K1033224) and CP44 (BBa_K1033225), and testing the newly formed RBS-NisA-double terminator (BBa_K1365556) and RBS-Superfolded GFP-double terminator (BBa_K1365555) BioBricks
 
The RBS-NisA-double terminator BioBrick was ligated with CP29 and CP44 from the promoter collection
The RBS-Superfolded GFP-double terminator BioBricks was ligated with all the promoters of the promoter collection