Team:Groningen/Template/MODULE/Notebook/characterisation/week2

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8 - 14 September
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September 8 - September 14
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Obtaining Nis A and Superfolded GFP in larger concentration for further use
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Obtaining the NisA (<a href="http://parts.igem.org/Part:BBa_K1365000">BBa_K1365000</a>) and the Superfolded GFP (<a href="http://parts.igem.org/Part:BBa_K1365020">BBa_K1365020</a>) BioBricks in larger concentration for further use
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<div class="item">Transformed BB PSBIC3 Nis A and BB psbic3 Superfolded GFP in <i>E.col</i></div>
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<div class="item">Transformed both, pSB1C3 containing the NisA BioBrick and pSB1C3 with the Superfolded GFP Biobrick, to two different <i>E. coli</i> DH5α strains</div>
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<div class="item">Bands were observed and were OD desired size, so the transformation was successful</div>
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<div class="item">The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful</div>
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<div class="item">Plasmid isolation and glycerol stock preparation</div>
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<div class="item">The plasmids with the desired BioBricks were isolated out of <i>E. coli</i> DH5α, and glycerol stocks were made of the two strains containing the previous named parts</div>
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Latest revision as of 21:32, 17 October 2014

September 8 - September 14
 
Obtaining the NisA (BBa_K1365000) and the Superfolded GFP (BBa_K1365020) BioBricks in larger concentration for further use
 
Transformed both, pSB1C3 containing the NisA BioBrick and pSB1C3 with the Superfolded GFP Biobrick, to two different E. coli DH5α strains
The transformations were checked on gel, and the desired bands were observed, therefore the transformation was most likely successful
The plasmids with the desired BioBricks were isolated out of E. coli DH5α, and glycerol stocks were made of the two strains containing the previous named parts
 
 
 
 
 

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