Team:Paris Bettencourt/Parts

From 2014.igem.org

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<tr>
<tr>
<td><b>SMELL THE ROSES</b></br>
<td><b>SMELL THE ROSES</b></br>
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<p class=text1><ul><li><a href="#part1">BBa_K1403003</a></li><li><a href="#part2">BBa_K1403006</a></li><li><href="#part3">BBa_K1403009</a></li><li><a href="#part4">BBa_K1403012</a></li><li><a href="#part5">BBa_K1403017</a></li><li><a href="#part6">BBa_K1403019</a></li></ul></p></td>
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<p class=text1><ul><li><a href="#part1">BBa_K1403003</a></li><li><a href="#part2">BBa_K1403006</a></li><li><a href="#part3">BBa_K1403009</a></li><li><a href="#part4">BBa_K1403012</a></li><li><a href="#part5">BBa_K1403017</a></li><li><a href="#part6">BBa_K1403019</a></li><li><a href="#part11">BBa_K1403013</a></li><li><a href="#part6">BBa_K1403019</a></li></ul></p></td>
<td><b>SOMETHING FISHY</b></br>
<td><b>SOMETHING FISHY</b></br>
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<p class=text1><ul><li><a href="#part7">BBa_K1403015</a></li></ul></br><b>DON'T SWEAT IT</b><ul><li><a href="#part8">BBa_K1403018</a></li></ul></p></td>
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<p class=text1><ul><li><a href="#part7">BBa_K1403015</a></li></ul></br>
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                        <td><b>DON'T SWEAT IT</b><p class=text1><ul><li><a href="#part8">BBa_K1403018</a></li></ul></p></td>
<td><b>INTERLAB STUDY</b></br>
<td><b>INTERLAB STUDY</b></br>
<p class=text1><ul><li><a href="#part9">BBa_K1403000</a></li><li><a href="#part10">BBa_K1403001</a></li></ul></p></td>
<p class=text1><ul><li><a href="#part9">BBa_K1403000</a></li><li><a href="#part10">BBa_K1403001</a></li></ul></p></td>
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</table>
</table>
         </div>
         </div>
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         <div id=project1><h6>Smell the roses</h6>
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         <div class=project><h6>Smell the roses</h6>
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         <div class=part id=part1><p><a href="http://parts.igem.org/Part:BBa_K1403003"><u>BBa_K1403003</a> - Limonene synthase</u></br></br>This part consists of a limonene synthase coding sequence downstream of a constitutive promoter.</br></br>Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent./p></div>
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         <div class=part id=part1><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403003"><u>BBa_K1403003</a> - Limonene synthase</u> Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.</p></div>
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         <div class=part id=part2><p><u><a href="http://parts.igem.org/Part:BBa_K1403006">BBa_K1403006</a> - Jasmonic acid carboxyl methyltransferase</u></br></br>Jasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid</p></div>
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         <div class=part id=part2><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403006">BBa_K1403006</a> - Jasmonic acid carboxyl methyltransferase</u> Jasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid.</p></div>
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  <div class=part id=part3><p><a href="http://parts.igem.org/Part:BBa_K1403009">BBa_K1403009</a> - <i>bmst1</i></br></br> BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell.</p> </div>
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  <div class=part id=part3><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403009">BBa_K1403009</a> - <i>bmst1</i></u> BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell. It laso produces floral smell (Methyl benzoate) from Benzoic acid.</p> </div>
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         <div class=part id=part4><p><u><a href="http://parts.igem.org/Part:BBa_K1403009">BBa_K1403009</a>BBa_K1403012</a> - alcohol acetyltransferase I</u></br></br>This enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor</p></div>
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         <div class=part id=part4><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403012">BBa_K1403012</a> - alcohol acetyltransferase I</u> This enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor.</p></div>
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    <div class=part id=part5><p>BBa_K1403017 - <i>aldB</i></br></br>It stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.</p></div>
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  <div class=part id=part5><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403013"><u>BBa_K1403013</a> - Germacradienol/germacrene D synthase</u> This enzyme produces Geosmin (rain smell) from farnesyl diphosphate.</p></div>
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         <div class=part id=part6><p>Part 6- ilvBN</br></br><i>ilvBN</i> gene exists in natural wild type E.Coli strain. They codes for acetolactate synthase process. Here, we isolated it from MG1655 strain through PCR to transform it into targeted strain for increased basal production of acetolactate, which serves as precursor for acetoin production.</p></div>
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        </div>   
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        </div>
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<div class=part id=part6><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403017"><u>BBa_K1403017</a> - <i>aldB</i></u> AldB stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.</p></div>
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         <div id=project2><h6>Something fishy</h6>
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         <div class=part id=part7><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403019"><u>BBa_K1403019</a> - <i>ilvBN</i></u> <i>ilvBN</i> gene encodes for acetolactate synthase process. We used it to increase basal production of acetolactate, which serves as precursor for acetoin production.</p></div>
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             <div class=part id=part7><p>Part 7</p></div>
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 +
         <div class=project><h6>Something fishy</h6>
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             <div class=part id=part7><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403015"><u>BBa_K1403015</a> - Trimethylamine monooxygenase</u> Trimethylamine monooxygenase enzyme degrades Trimethylamine (fish odor) into non-smelly compund. It also produces indigo (blue pigment) form  tryptophan.</p></div>
         </div>
         </div>
    
    
         </div>
         </div>
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         <div id=project3><h6>Don't sweat it</h6>
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         <div class=project><h6>Don't sweat it</h6>
    
    
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         <div class=part id=part8><p>Part 8</p></div>
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         <div class=part id=part8><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403015">BBa_K1403018 - <i>agaA</i></a></u> <i>AgaA</i> is a gene present in Corynebacterium striatum and it is the main responsible for body odor. It transforms 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid which smells like sweat.</p></div>
         </div>
         </div>
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  <div id=project4><h6>Interlab study</h6>
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  <div class=project><h6>Interlab study</h6>
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   <div class=part id=part9><p>Part 9</p></div>
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   <div class=part id=part9><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> - GFP under strong promoter in pSB1C3</u> Measurment kit test of Part:<a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a> in pSB1C3. This part is a tween of <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> but in a high copy pSB1C3 plasmid. </p></div>
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</div>
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  <div class=part id=part10><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a>BBa_K1403000</a> - GFP generator under a weak promoter in pSB1C3</u> Measurment kit test of <a href="http://parts.igem.org/Part:BBa_J23115">BBa_J23115</a> in pSB1C3. It is placed in a high coply plasmid,the GFP expression is under a weak Anderson promoter.</p></div>
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  <div class=part id=part10><p>Part 10</p></div>
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Latest revision as of 03:52, 18 October 2014

Smell the roses

BBa_K1403003 - Limonene synthase Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

BBa_K1403006 - Jasmonic acid carboxyl methyltransferase Jasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid.

BBa_K1403009 - bmst1 BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell. It laso produces floral smell (Methyl benzoate) from Benzoic acid.

BBa_K1403012 - alcohol acetyltransferase I This enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor.

BBa_K1403013 - Germacradienol/germacrene D synthase This enzyme produces Geosmin (rain smell) from farnesyl diphosphate.

BBa_K1403017 - aldB AldB stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.

BBa_K1403019 - ilvBN ilvBN gene encodes for acetolactate synthase process. We used it to increase basal production of acetolactate, which serves as precursor for acetoin production.

Something fishy

BBa_K1403015 - Trimethylamine monooxygenase Trimethylamine monooxygenase enzyme degrades Trimethylamine (fish odor) into non-smelly compund. It also produces indigo (blue pigment) form tryptophan.

Don't sweat it

BBa_K1403018 - agaA AgaA is a gene present in Corynebacterium striatum and it is the main responsible for body odor. It transforms 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid which smells like sweat.

Interlab study

BBa_K1403000 - GFP under strong promoter in pSB1C3 Measurment kit test of Part:BBa_J23101 in pSB1C3. This part is a tween of BBa_I20260 but in a high copy pSB1C3 plasmid.

BBa_J23101BBa_K1403000 - GFP generator under a weak promoter in pSB1C3 Measurment kit test of BBa_J23115 in pSB1C3. It is placed in a high coply plasmid,the GFP expression is under a weak Anderson promoter.

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
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