"
Team:Bordeaux/Parts/BBa K1317004
From 2014.igem.org
m |
m |
||
| (2 intermediate revisions not shown) | |||
| Line 5: | Line 5: | ||
[[File:Bdx2014 ELP-RFP.PNG|800px|thumb|left]] | [[File:Bdx2014 ELP-RFP.PNG|800px|thumb|left]] | ||
| - | MAP of ELP-RLP<br /> | + | MAP of ELP-RLP in pSB1C3. The final assembling could not be performed because we did not have the time to do it.<br /> |
| + | <br /> | ||
| + | |||
| + | The ELP presents specific properties: it precipitates at high temperature. This property can be used for thermal cycling purification for instance. By successive cycles of temperature, ELP can be purified from a mix of proteins. This special property has been used to create a composite part for the iGEM registry. It is a proof of concept of the use of this part as a tool for protein purification by fusing ELP to another protein. The protein used as an example is RFP, to ease vizualisation of the process.<br /> | ||
| + | |||
| + | This part is a composite part made of BBa_K1317003 and BBa_E1010 (highly engineered RFP) | ||
<br /> | <br /> | ||
<br /> | <br /> | ||
Latest revision as of 02:46, 18 October 2014
Link to the registry of Standard Parts: [http://parts.igem.org/Part:BBa_K1317004 http://parts.igem.org/Part:BBa_K1317004]
MAP of ELP-RLP in pSB1C3. The final assembling could not be performed because we did not have the time to do it.
The ELP presents specific properties: it precipitates at high temperature. This property can be used for thermal cycling purification for instance. By successive cycles of temperature, ELP can be purified from a mix of proteins. This special property has been used to create a composite part for the iGEM registry. It is a proof of concept of the use of this part as a tool for protein purification by fusing ELP to another protein. The protein used as an example is RFP, to ease vizualisation of the process.
This part is a composite part made of BBa_K1317003 and BBa_E1010 (highly engineered RFP)
