Team:Paris Bettencourt/Parts

From 2014.igem.org

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<tr>
<tr>
<td><b>SMELL THE ROSES</b></br>
<td><b>SMELL THE ROSES</b></br>
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<p class=text1><ul><li><a href="#part1">BBa_K1403003</a></li><li><a href="#part2">BBa_K1403006</a></li><li><href="#part3">BBa_K1403009</a></li><li><a href="#part4">BBa_K1403012</a></li><li><a href="#part5">BBa_K1403017</a></li><li><a href="#part6">BBa_K1403019</a></li></ul></p></td>
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<p class=text1><ul><li><a href="#part1">BBa_K1403003</a></li><li><a href="#part2">BBa_K1403006</a></li><li><a href="#part3">BBa_K1403009</a></li><li><a href="#part4">BBa_K1403012</a></li><li><a href="#part5">BBa_K1403017</a></li><li><a href="#part6">BBa_K1403019</a></li><li><a href="#part11">BBa_K1403013</a></li><li><a href="#part6">BBa_K1403019</a></li></ul></p></td>
<td><b>SOMETHING FISHY</b></br>
<td><b>SOMETHING FISHY</b></br>
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<p class=text1><ul><li><a href="#part7">BBa_K1403015</a></li></ul></br><b>DON'T SWEAT IT</b><ul><li><a href="#part8">BBa_K1403018</a></li></ul></p></td>
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<p class=text1><ul><li><a href="#part7">BBa_K1403015</a></li></ul></br>
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                        <td><b>DON'T SWEAT IT</b><p class=text1><ul><li><a href="#part8">BBa_K1403018</a></li></ul></p></td>
<td><b>INTERLAB STUDY</b></br>
<td><b>INTERLAB STUDY</b></br>
<p class=text1><ul><li><a href="#part9">BBa_K1403000</a></li><li><a href="#part10">BBa_K1403001</a></li></ul></p></td>
<p class=text1><ul><li><a href="#part9">BBa_K1403000</a></li><li><a href="#part10">BBa_K1403001</a></li></ul></p></td>
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         <div id=project1><h6>Smell the roses</h6>
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         <div class=project><h6>Smell the roses</h6>
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         <div class=part id=part1><p><a href="http://parts.igem.org/Part:BBa_K1403003">BBa_K1403003</a> - Limonene synthase</p><hr></br>This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. We added a promoter and a RBS site to the original part Bba_I742110, making it directly expressed in E.Coli. </br></br>The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library.</br></br>
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         <div class=part id=part1><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403003"><u>BBa_K1403003</a> - Limonene synthase</u> Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.</p></div>
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        <div class=part id=part2><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403006">BBa_K1403006</a> - Jasmonic acid carboxyl methyltransferase</u> Jasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid.</p></div>
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<div class=part id=part3><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403009">BBa_K1403009</a> - <i>bmst1</i></u> BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell. It laso produces floral smell (Methyl benzoate) from Benzoic acid.</p> </div>
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        <div class=part id=part4><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403012">BBa_K1403012</a> - alcohol acetyltransferase I</u> This enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor.</p></div>
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  <div class=part id=part5><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403013"><u>BBa_K1403013</a> - Germacradienol/germacrene D synthase</u> This enzyme produces Geosmin (rain smell) from farnesyl diphosphate.</p></div>
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        </div>   
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<div class=part id=part6><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403017"><u>BBa_K1403017</a> - <i>aldB</i></u> AldB stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.</p></div>
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        <div class=part id=part7><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403019"><u>BBa_K1403019</a> - <i>ilvBN</i></u> <i>ilvBN</i> gene encodes for acetolactate synthase process. We used it to increase basal production of acetolactate, which serves as precursor for acetoin production.</p></div>
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We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later. Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.</br></br>
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         <div class=project><h6>Something fishy</h6>
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            <div class=part id=part7><p class=text1><a target="blank" href="http://parts.igem.org/Part:BBa_K1403015"><u>BBa_K1403015</a> - Trimethylamine monooxygenase</u> Trimethylamine monooxygenase enzyme degrades Trimethylamine (fish odor) into non-smelly compund. It also produces indigo (blue pigment) form  tryptophan.</p></div>
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The experiment process was completed by standard cloning. The promoter and RBS was designed as oligos and later aligned, digested and ligated. The finished plasmid is in standard biobricks format. </p></div>
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         <div class=part id=part2><p>Part 2</p></div>
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<div class=part id=part3><p>Part 3</p></div>
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        <div class=part id=part4><p>Part 4</p></div>
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    <div class=part id=part5><p>Part 5</p></div>
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        <div class=part id=part6><p>Part 6</p></div>
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        </div>
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        <div id=project2><h6>Something fishy</h6>
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            <div class=part id=part7><p>Part 7</p></div>
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         </div>
         </div>
    
    
         </div>
         </div>
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         <div id=project3><h6>Don't sweat it</h6>
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         <div class=project><h6>Don't sweat it</h6>
    
    
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         <div class=part id=part8><p>Part 8</p></div>
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         <div class=part id=part8><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403015">BBa_K1403018 - <i>agaA</i></a></u> <i>AgaA</i> is a gene present in Corynebacterium striatum and it is the main responsible for body odor. It transforms 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid which smells like sweat.</p></div>
         </div>
         </div>
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  <div id=project4><h6>Interlab study</h6>
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  <div class=project><h6>Interlab study</h6>
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   <div class=part id=part9><p>Part 9</p></div>
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   <div class=part id=part9><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_K1403000">BBa_K1403000</a> - GFP under strong promoter in pSB1C3</u> Measurment kit test of Part:<a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a> in pSB1C3. This part is a tween of <a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> but in a high copy pSB1C3 plasmid. </p></div>
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</div>
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  <div class=part id=part10><p class=text1><u><a target="blank" href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a>BBa_K1403000</a> - GFP generator under a weak promoter in pSB1C3</u> Measurment kit test of <a href="http://parts.igem.org/Part:BBa_J23115">BBa_J23115</a> in pSB1C3. It is placed in a high coply plasmid,the GFP expression is under a weak Anderson promoter.</p></div>
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  <div class=part id=part10><p>Part 10</p></div>
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Latest revision as of 03:52, 18 October 2014

Smell the roses

BBa_K1403003 - Limonene synthase Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

BBa_K1403006 - Jasmonic acid carboxyl methyltransferase Jasmonic acid carboxyl methyltransferase catalyzes the formation of methyl jasmonate from jasmonic acid.

BBa_K1403009 - bmst1 BMST1 enzyme that catalyzes production of methyl salicylate from salicylic acid. Methyl salicylate has a wintergreen smell. It laso produces floral smell (Methyl benzoate) from Benzoic acid.

BBa_K1403012 - alcohol acetyltransferase I This enzyme converts isoamyl alcohol to isoamyl acetate producing banana odor.

BBa_K1403013 - Germacradienol/germacrene D synthase This enzyme produces Geosmin (rain smell) from farnesyl diphosphate.

BBa_K1403017 - aldB AldB stimulates acetolactate decarboxylase activities to transform acetolactate to acetoin, which smells like butter.

BBa_K1403019 - ilvBN ilvBN gene encodes for acetolactate synthase process. We used it to increase basal production of acetolactate, which serves as precursor for acetoin production.

Something fishy

BBa_K1403015 - Trimethylamine monooxygenase Trimethylamine monooxygenase enzyme degrades Trimethylamine (fish odor) into non-smelly compund. It also produces indigo (blue pigment) form tryptophan.

Don't sweat it

BBa_K1403018 - agaA AgaA is a gene present in Corynebacterium striatum and it is the main responsible for body odor. It transforms 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid which smells like sweat.

Interlab study

BBa_K1403000 - GFP under strong promoter in pSB1C3 Measurment kit test of Part:BBa_J23101 in pSB1C3. This part is a tween of BBa_I20260 but in a high copy pSB1C3 plasmid.

BBa_J23101BBa_K1403000 - GFP generator under a weak promoter in pSB1C3 Measurment kit test of BBa_J23115 in pSB1C3. It is placed in a high coply plasmid,the GFP expression is under a weak Anderson promoter.

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
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paris-bettencourt-igem@googlegroups.com
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