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Team:ETH Zurich/expresults/qs/tab-prhl
From 2014.igem.org
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| - | |+'''Table | + | |+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017]) |
| - | |style="width:200px"|[[File:ETH_Zurich_2014_qs- | + | |
| + | |colspan="4" style='font-size:10pt';text-align:left| | ||
| + | In this set of experiments the promoter [http://parts.igem.org/Part:BBa_R0071 pRhl] was tested for potential crosstalk. In the top left position we observe the induction of [http://parts.igem.org/Part:BBa_R0071 pRhl] by C4-HSL bound to the [http://parts.igem.org/Part:BBa_C0171 regulator RhlR]. The switching behaviour was observed at a C4-HSL concentration of 1 μM. | ||
| + | In the case of 3OC12-HSL binding the [http://parts.igem.org/Part:BBa_C0171 RhlR regulator] and subsequently the [http://parts.igem.org/Part:BBa_R0071 promoter pRhl]insignificant crosstalk has been observed. | ||
| + | Severe crosstalk was observed in the case of 3OC6-HSL binding the [http://parts.igem.org/Part:BBa_C0171 RhlR regulator] followed by induction of [http://parts.igem.org/Part:BBa_R0071 pRhl]. The transition occurred at a concentration of the inducer molecule of 1 μM but compared to the reference curve a lower value of fluorescence per OD was observed (1000 a.u.). Another case of crosstalk with the [http://parts.igem.org/Part:BBa_R0071 pRhl] was detected with 3OC12-HSL binding to the corresponding [http://parts.igem.org/Part:BBa_C0179 LasR regulator] followed by inducing the promoter [http://parts.igem.org/Part:BBa_R0071 pRhl]. Here switching occurred at a concentration 1 nM of 3OC12-HSL and reached fluorescence per OD of 750 a.u.. This is approximately 0.5 fold the value of the fluorescence per OD shown by the reference curve indicated in green. | ||
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| + | |style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerRhl.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]] | ||
|[[File:ETH_Zurich_2014_qs-table_C4-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]] | |[[File:ETH_Zurich_2014_qs-table_C4-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]] | ||
|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]] | |[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]] | ||
|[[File:ETH_Zurich_2014_qs-table_3OC12-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]] | |[[File:ETH_Zurich_2014_qs-table_3OC12-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]] | ||
|- | |- | ||
| - | |[[File:ETH_Zurich_2014_qs- | + | |[[File:ETH_Zurich_2014_qs-table_RhlR.png|200px|link=http://parts.igem.org/Part:BBa_C0171:Experience]] |
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|[[File:ETH_Zurich_2014_qs-table_PrhlRef.png|300px]] | |[[File:ETH_Zurich_2014_qs-table_PrhlRef.png|300px]] | ||
| + | |[[File:ETH_Zurich_2014_qs-table_PrhlRhlRLuxAHL.png|300px]] | ||
| + | |[[File:ETH_Zurich_2014_qs-table_PrhlRhlRLasAHL.png|300px]] | ||
| + | |- | ||
| + | |[[File:ETH_Zurich_2014_qs-table_LuxR.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]] | ||
| + | |[[File:ETH_Zurich_2014_qs-table_PrhlLuxRRhlAHL.png|300px]] | ||
| + | |[[File:ETH_Zurich_2014_qs-table_PrhlLuxRLuxAHL.png|300px]] | ||
|[[File:ETH_Zurich_2014_qs-table_PrhlLuxRLasAHL.png|300px]] | |[[File:ETH_Zurich_2014_qs-table_PrhlLuxRLasAHL.png|300px]] | ||
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|[[File:ETH_Zurich_2014_qs-table_PrhlLasRLuxAHL.png|300px]] | |[[File:ETH_Zurich_2014_qs-table_PrhlLasRLuxAHL.png|300px]] | ||
|[[File:ETH_Zurich_2014_qs-table_PrhlLasRLasAHL.png|300px]] | |[[File:ETH_Zurich_2014_qs-table_PrhlLasRLasAHL.png|300px]] | ||
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Latest revision as of 22:54, 17 October 2014
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In this set of experiments the promoter [http://parts.igem.org/Part:BBa_R0071 pRhl] was tested for potential crosstalk. In the top left position we observe the induction of [http://parts.igem.org/Part:BBa_R0071 pRhl] by C4-HSL bound to the [http://parts.igem.org/Part:BBa_C0171 regulator RhlR]. The switching behaviour was observed at a C4-HSL concentration of 1 μM. In the case of 3OC12-HSL binding the [http://parts.igem.org/Part:BBa_C0171 RhlR regulator] and subsequently the [http://parts.igem.org/Part:BBa_R0071 promoter pRhl]insignificant crosstalk has been observed. Severe crosstalk was observed in the case of 3OC6-HSL binding the [http://parts.igem.org/Part:BBa_C0171 RhlR regulator] followed by induction of [http://parts.igem.org/Part:BBa_R0071 pRhl]. The transition occurred at a concentration of the inducer molecule of 1 μM but compared to the reference curve a lower value of fluorescence per OD was observed (1000 a.u.). Another case of crosstalk with the [http://parts.igem.org/Part:BBa_R0071 pRhl] was detected with 3OC12-HSL binding to the corresponding [http://parts.igem.org/Part:BBa_C0179 LasR regulator] followed by inducing the promoter [http://parts.igem.org/Part:BBa_R0071 pRhl]. Here switching occurred at a concentration 1 nM of 3OC12-HSL and reached fluorescence per OD of 750 a.u.. This is approximately 0.5 fold the value of the fluorescence per OD shown by the reference curve indicated in green. | |||
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