Team:ETH Zurich/expresults/qs/tab-prhl

From 2014.igem.org

(Difference between revisions)

Latest revision as of 22:54, 17 October 2014

**Table 3** Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])
In this set of experiments the promoter [http://parts.igem.org/Part:BBa_R0071 pRhl] was tested for potential crosstalk. In the top left position we observe the induction of [http://parts.igem.org/Part:BBa_R0071 pRhl] by C4-HSL bound to the [http://parts.igem.org/Part:BBa_C0171 regulator RhlR]. The switching behaviour was observed at a C4-HSL concentration of 1 μM. In the case of 3OC12-HSL binding the [http://parts.igem.org/Part:BBa_C0171 RhlR regulator] and subsequently the [http://parts.igem.org/Part:BBa_R0071 promoter pRhl]insignificant crosstalk has been observed. Severe crosstalk was observed in the case of 3OC6-HSL binding the [http://parts.igem.org/Part:BBa_C0171 RhlR regulator] followed by induction of [http://parts.igem.org/Part:BBa_R0071 pRhl]. The transition occurred at a concentration of the inducer molecule of 1 μM but compared to the reference curve a lower value of fluorescence per OD was observed (1000 a.u.). Another case of crosstalk with the [http://parts.igem.org/Part:BBa_R0071 pRhl] was detected with 3OC12-HSL binding to the corresponding [http://parts.igem.org/Part:BBa_C0179 LasR regulator] followed by inducing the promoter [http://parts.igem.org/Part:BBa_R0071 pRhl]. Here switching occurred at a concentration 1 nM of 3OC12-HSL and reached fluorescence per OD of 750 a.u.. This is approximately 0.5 fold the value of the fluorescence per OD shown by the reference curve indicated in green.

@@ Line 1: / Line 1: @@
 {|class="wikitable" style="background-color: white; text-align:center; width:auto; margin: auto;"
-|+'''Table 1''' Crosstalk matrix for the promoter plux ([http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062])
+|+'''Table 3''' Crosstalk matrix for the promoter prhl ([http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017])
-|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_Corner.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]
-|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]
+|colspan="4" style='font-size:10pt';text-align:left|
-|[[File:ETH_Zurich_2014_qs-table_3OC12-HSL.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]
+In this set of experiments the promoter [http://parts.igem.org/Part:BBa_R0071 pRhl] was tested for potential crosstalk. In the top left position we observe the induction of [http://parts.igem.org/Part:BBa_R0071 pRhl] by C4-HSL bound to the [http://parts.igem.org/Part:BBa_C0171 regulator RhlR]. The switching behaviour was observed at a C4-HSL concentration of 1 μM.
-|[[File:ETH_Zurich_2014_qs-table_C4-HSL.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]
+In the case of 3OC12-HSL binding the [http://parts.igem.org/Part:BBa_C0171 RhlR regulator] and subsequently the [http://parts.igem.org/Part:BBa_R0071 promoter pRhl]insignificant crosstalk has been observed.
+Severe crosstalk was observed in the case of 3OC6-HSL binding the [http://parts.igem.org/Part:BBa_C0171 RhlR regulator] followed by induction of [http://parts.igem.org/Part:BBa_R0071 pRhl]. The transition occurred at a concentration of the inducer molecule of 1 μM but compared to the reference curve a lower value of fluorescence per OD was observed (1000 a.u.). Another case of crosstalk with the [http://parts.igem.org/Part:BBa_R0071 pRhl] was detected with 3OC12-HSL binding to the corresponding [http://parts.igem.org/Part:BBa_C0179 LasR regulator] followed by inducing the promoter [http://parts.igem.org/Part:BBa_R0071 pRhl]. Here switching occurred at a concentration 1 nM of 3OC12-HSL and reached fluorescence per OD of 750 a.u.. This is approximately 0.5 fold the value of the fluorescence per OD shown by the reference curve indicated in green.
+|-
+|style="width:200px"|[[File:ETH_Zurich_2014_qs-table_CornerRhl.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]
+|[[File:ETH_Zurich_2014_qs-table_C4-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]
+|[[File:ETH_Zurich_2014_qs-table_3OC6-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]
+|[[File:ETH_Zurich_2014_qs-table_3OC12-HSL.png|200px|link=http://parts.igem.org/Part:BBa_I14017:Experience]]
+|-
+|[[File:ETH_Zurich_2014_qs-table_RhlR.png|200px|link=http://parts.igem.org/Part:BBa_C0171:Experience]]
+|[[File:ETH_Zurich_2014_qs-table_PrhlRef.png|300px]]
+|[[File:ETH_Zurich_2014_qs-table_PrhlRhlRLuxAHL.png|300px]]
+|[[File:ETH_Zurich_2014_qs-table_PrhlRhlRLasAHL.png|300px]]
 |-
 |[[File:ETH_Zurich_2014_qs-table_LuxR.png|200px|link=http://parts.igem.org/Part:BBa_C0062:Experience]]
-|[[File:ETH_Zurich_2014_qs-table_PluxRef.png|300px]]
+|[[File:ETH_Zurich_2014_qs-table_PrhlLuxRRhlAHL.png|300px]]
-|[[File:ETH_Zurich_2014_qs-table_PluxLuxRLasAHL.png|300px]]
+|[[File:ETH_Zurich_2014_qs-table_PrhlLuxRLuxAHL.png|300px]]
-|[[File:ETH_Zurich_2014_qs-table_PluxLuxRRhlAHL.png|300px]]
+|[[File:ETH_Zurich_2014_qs-table_PrhlLuxRLasAHL.png|300px]]
 |-
 |[[File:ETH_Zurich_2014_qs-table_LasR.png|200px|link=http://parts.igem.org/Part:BBa_C0179:Experience]]
-|[[File:ETH_Zurich_2014_qs-table_PluxLasRLuxAHL.png|300px]]
+|[[File:ETH_Zurich_2014_qs-table_PrhlLasRRhlAHL.png|300px]]
-|[[File:ETH_Zurich_2014_qs-table_PluxLasRLasAHL.png|300px]]
+|[[File:ETH_Zurich_2014_qs-table_PrhlLasRLuxAHL.png|300px]]
-|[[File:ETH_Zurich_2014_qs-table_PluxLasRRhlAHL.png|300px]]
+|[[File:ETH_Zurich_2014_qs-table_PrhlLasRLasAHL.png|300px]]
-|-
-|[[File:ETH_Zurich_2014_qs-table_RhlR.png|200px|link=http://parts.igem.org/Part:BBa_C0171:Experience]]
-|[[File:ETH_Zurich_2014_qs-table_PluxRhlRLuxAHL.png|300px]]
-|[[File:ETH_Zurich_2014_qs-table_PluxRhlRLasAHL.png|300px]]
-|[[File:ETH_Zurich_2014_qs-table_PluxRhlRRhlAHL.png|300px]]
 |}